File:Defined-Essential-8™-Medium-and-Vitronectin-Efficiently-Support-Scalable-Xeno-Free-Expansion-of-pone.0151264.s005.ogv

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Description
English: Human iPSCs (Gibco hiPSC line) cultured in spinner flasks with E8 medium and VtnB retain their differentiation potential. Cardiomyocyte differentiation was performed using Life Technologies Cardiomyocytes Differentiation Kit by plating microcarriers with hiPS cells in low-attachment plates. Beating cell-VtnM aggregates in low-attachment plate were observed at day 10 of differentiation.
Date
Source S2 Video from Badenes S, Fernandes T, Cordeiro C, Boucher S, Kuninger D, Vemuri M, Diogo M, Cabral J (2016). "Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems". PLOS ONE. DOI:10.1371/journal.pone.0151264. PMID 27149065. PMC: 4801338.
Author Badenes S, Fernandes T, Cordeiro C, Boucher S, Kuninger D, Vemuri M, Diogo M, Cabral J
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21 March 2016

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