Carnoy's solution

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Carnoy's solution is a fixative composed of 60% ethanol, 30% chloroform and 10% glacial acetic acid, 1 gram of ferric chloride.[1][2]

Carnoy's solution is also the name of a different fixation composed of ethanol and glacial acetic acid (3:1).[3][4][5]

The invention of Carnoy's solution is attributed to Jean-Baptiste Carnoy, a pioneering 19th century cytologist.[6]

Uses

Some of the uses of Carnoy's solution are:

  • Enhancing lymph node detection during dissection of cadavers.[7]
  • Immunohistochemical fixation and detection of NMDA receptors within the murine hippocampus.[8]
  • Applied directly following enucleation for the treatment of odontogenic keratocysts.[9][10][11]
  • Direct application following enucleation (Cuba) for certain kinds of unicystic ameloblastomas.[12] This appears to decrease the likelihood of recurrence over enucleation alone.[13] Protein coagulation is thought to limit uptake of these toxic materials by surrounding tissues, however it is this fact that limits its usefulness as a treatment agent in general.[14]
  • As a fixative for pap smear samples.[15]
  • As a fixative agent for both nuclear and mitochondrial DNA in various tissues.[16]
  • As a fixative agent to preserve mucus, useful for tissue preparation before staining with periodic acid-Schiff base.[17]

References

  1. ↑ "MSDS :: Carnoy's Solution (Fixative)". Archived from the original on 2009-03-27. https://web.archive.org/web/20090327061520/http://www.sciencestuff.com/msds/C1466.html. Retrieved 13 Jan 2009. 
  2. ↑ Carnoy J. B. (1887). "Appendice Les Globule Polaires de L'Ascaris Clavata". La Cellule RECUEIL DE CYTOLOGIE ET d'HISTOLOGIE GÉNÉRALE 3: 276. https://www.biodiversitylibrary.org/page/6937354. 
  3. ↑ "Chromosome Preparatons of Bone Marrow Cells without PriorIn VitroCulture orIn VivoColchicine Administration". Stain Technology 37: 17–20. 1962. doi:10.3109/10520296209114563. PMID 13921436. 
  4. ↑ "The Cytochemical Staining and Measurement of Protein with Mercuric Bromphenol Blue". The Biological Bulletin 104 (1): 57–67. 1953. doi:10.2307/1538691. https://www.biodiversitylibrary.org/part/32681. 
  5. ↑ "Chromosome numbers for three species of medicinal leeches (Hirudo spp.)". Systematic Parasitology 74 (2): 95–102. 2009. doi:10.1007/s11230-009-9198-2. PMID 19731093. 
  6. ↑ Ireland, Robert; Yeung, Chuen Albert YeungChuen Albert (2020-04-23), Yeung, Chuen Albert, ed., "Carnoy’s solution" (in en), A Dictionary of Dentistry (Oxford University Press), doi:10.1093/acref/9780191828621.001.0001/acref-9780191828621-e-5108;jsessionid=ea246f7ff0d191c9a7457e5a9b9974b3, ISBN 978-0-19-182862-1, https://www.oxfordreference.com/display/10.1093/acref/9780191828621.001.0001/acref-9780191828621-e-5108;jsessionid=EA246F7FF0D191C9A7457E5A9B9974B3, retrieved 2023-04-10 
  7. ↑ "Carnoy's solution enhances lymph node detection: an anatomical dissection study in cadavers". Histopathology 53 (6): 740–2. December 2008. doi:10.1111/j.1365-2559.2008.03148.x. PMID 19076686. 
  8. ↑ "Immersion fixation with Carnoy solution for conventional immunohistochemical detection of particular N-methyl-D-aspartate receptor subunits in murine hippocampus". J. Neurosci. Res. 73 (3): 416–26. August 2003. doi:10.1002/jnr.10622. PMID 12868075. 
  9. ↑ "Keratocystic odontogenic tumour: reclassification of the odontogenic keratocyst from cyst to tumour". J Can Dent Assoc 74 (2): 165–165h. March 2008. PMID 18353202. http://www.cda-adc.ca/jcda/vol-74/issue-2/165.html. 
  10. ↑ "Odontogenic Keratocyst: The Northwestern USA Experience". Archived from the original on 2009-01-06. https://web.archive.org/web/20090106171206/http://www.thejcdp.com/issue002/oda/04oda.htm. Retrieved 14 Jan 2009. 
  11. ↑ "Use of Carnoy's Solution in management of odontogenic keratocysts". Archived from the original on 2009-05-17. https://web.archive.org/web/20090517020923/http://iadr.confex.com/iadr/2006Orld/techprogram/abstract_76521.htm. Retrieved 14 Jan 2009. 
  12. ↑ "Unicystic ameloblastoma--use of Carnoy's solution after enucleation". Int J Oral Maxillofac Surg 33 (3): 263–7. April 2004. doi:10.1006/ijom.2003.0496. PMID 15290793. 
  13. ↑ "Recurrence related to treatment modalities of unicystic ameloblastoma: a systematic review". Int J Oral Maxillofac Surg 35 (8): 681–90. August 2006. doi:10.1016/j.ijom.2006.02.016. PMID 16782308. 
  14. ↑ Marx, Robert E.; Stern, Diane (2003). Oral and maxillofacial pathology: a rationale for diagnosis and treatment. Chicago: Quintessence. p. 684. ISBN 0-86715-390-3. https://archive.org/details/oralmaxillofacia00marx. 
  15. ↑ "Comparison of Carnoy's solution and 96% ethyl alcohol fixation in bloody Pap smears". Acta Cytol. 52 (2): 187–90. 2008. doi:10.1159/000325477. PMID 18499991. 
  16. ↑ "Effect of fixation to the degradation of nuclear and mitochondrial DNA in different tissues". J. Histochem. Cytochem. 54 (3): 371–4. March 2006. doi:10.1369/jhc.5B6726.2005. PMID 16260588. 
  17. ↑ "Stains File: Carnoy's Fluid". http://stainsfile.info/StainsFile/prepare/fix/fixatives/carnoy.htm. Retrieved 25 Oct 2009. 



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