Biology:Gene knockdown

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Gene knockdown is an experimental technique by which the expression of one or more of an organism's genes is reduced. The reduction can occur either through genetic modification or by treatment with a reagent such as a short DNA or RNA oligonucleotide that has a sequence complementary to either gene or an mRNA transcript.[1]

Versus transient knockdown

If a DNA of an organism is genetically modified, the resulting organism is called a "knockdown organism." If the change in gene expression is caused by an oligonucleotide binding to an mRNA or temporarily binding to a gene, this leads to a temporary change in gene expression that does not modify the chromosomal DNA, and the result is referred to as a "transient knockdown".[1]

In a transient knockdown, the binding of this oligonucleotide to the active gene or its transcripts causes decreased expression through a variety of processes. Binding can occur either through the blocking of transcription (in the case of gene-binding), the degradation of the mRNA transcript (e.g. by small interfering RNA (siRNA)) or RNase-H dependent antisense, or through the blocking of either mRNA translation, pre-mRNA splicing sites, or nuclease cleavage sites used for maturation of other functional RNAs, including miRNA (e.g. by morpholino oligos or other RNase-H independent antisense).[1][2]

The most direct use of transient knockdowns is for learning about a gene that has been sequenced, but has an unknown or incompletely known function. This experimental approach is known as reverse genetics. Researchers draw inferences from how the knockdown differs from individuals in which the gene of interest is operational. Transient knockdowns are often used in developmental biology because oligos can be injected into single-celled zygotes and will be present in the daughter cells of the injected cell through embryonic development.[3] The term gene knockdown first appeared in the literature in 1994[4]

RNA interference

RNA interference (RNAi) is a means of silencing genes by way of mRNA degradation.[5] Gene knockdown by this method is achieved by introducing small double-stranded interfering RNAs (siRNA) into the cytoplasm. Small interfering RNAs can originate from inside the cell or can be exogenously introduced into the cell. Once introduced into the cell, exogenous siRNAs are processed by the RNA-induced silencing complex (RISC).[6] The siRNA is complementary to the target mRNA to be silenced, and the RISC uses the siRNA as a template for locating the target mRNA. After the RISC localizes to the target mRNA, the RNA is cleaved by a ribonuclease.

RNAi is widely used as a laboratory technique for genetic functional analysis.[7] RNAi in organisms such as C. elegans and Drosophila melanogaster provides a quick and inexpensive means of investigating gene function. In C. elegans research, the availability of tools such as the Ahringer RNAi Library give laboratories a way of testing many genes in a variety of experimental backgrounds. Insights gained from experimental RNAi use may be useful in identifying potential therapeutic targets, drug development, or other applications.[8] RNA interference is a very useful research tool, allowing investigators to carry out large genetic screens in an effort to identify targets for further research related to a particular pathway, drug, or phenotype.[9][10]

CRISPRs

A different means of silencing exogenous DNA that has been discovered in prokaryotes is a mechanism involving loci called 'Clustered Regularly Interspaced Short Palindromic Repeats', or CRISPRs.[11] CRISPR-associated (cas) genes encode cellular machinery that cuts exogenous DNA into small fragments and inserts them into a CRISPR repeat locus. When this CRISPR region of DNA is expressed by the cell, the small RNAs produced from the exogenous DNA inserts serve as a template sequence that other Cas proteins use to silence this same exogenous sequence. The transcripts of the short exogenous sequences are used as a guide to silence these foreign DNA when they are present in the cell. This serves as a kind of acquired immunity, and this process is like a prokaryotic RNA interference mechanism. The CRISPR repeats are conserved amongst many species and have been demonstrated to be usable in human cells,[12] bacteria,[13] C. elegans,[14] zebrafish,[15] and other organisms for effective genome manipulation. The use of CRISPRs as a versatile research tool can be illustrated[16] by many studies making use of it to generate organisms with genome alterations.

TALENs

Another technology made possible by prokaryotic genome manipulation is the use of transcription activator-like effector nucleases (TALENs) to target specific genes.[17] TALENs are nucleases that have two important functional components: a DNA binding domain and a DNA cleaving domain. The DNA binding domain is a sequence-specific transcription activator-like effector sequence while the DNA cleaving domain originates from a bacterial endonuclease and is non-specific. TALENs can be designed to cleave a sequence specified by the sequence of the transcription activator-like effector portion of the construct. Once designed, a TALEN is introduced into a cell as a plasmid or mRNA. The TALEN is expressed, localizes to its target sequence, and cleaves a specific site. After cleavage of the target DNA sequence by the TALEN, the cell uses non-homologous end joining as a DNA repair mechanism to correct the cleavage. The cell's attempt at repairing the cleaved sequence can render the encoded protein non-functional, as this repair mechanism introduces insertion or deletion errors at the repaired site.

Commercialization

So far, knockdown organisms with permanent alterations in their DNA have been engineered chiefly for research purposes. Also known simply as knockdowns, these organisms are most commonly used for reverse genetics, especially in species such as mice or rats for which transient knockdown technologies cannot easily be applied.[3][18]

There are several companies that offer commercial services related to gene knockdown treatments.

See also

References

  1. ↑ 1.0 1.1 1.2 "Morpholino, siRNA, and S-DNA compared: impact of structure and mechanism of action on off-target effects and sequence specificity". Current Topics in Medicinal Chemistry 7 (7): 651–60. 2007. doi:10.2174/156802607780487740. PMID 17430206. 
  2. ↑ "Morpholino antisense oligomers: the case for an RNase H-independent structural type". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1489 (1): 141–58. December 1999. doi:10.1016/S0167-4781(99)00150-5. PMID 10807004. 
  3. ↑ 3.0 3.1 "Effective targeted gene 'knockdown' in zebrafish". Nature Genetics 26 (2): 216–20. October 2000. doi:10.1038/79951. PMID 11017081. 
  4. ↑ Wahlestedt, Claes (February 1994). "Antisense oligonucleotide strategies in neuropharmacology". Trends Pharmacol Sci 15 (2): 42–6. doi:10.1016/0165-6147(94)90107-4. PMID 8165722. 
  5. ↑ "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans". Nature 391 (6669): 806–11. February 1998. doi:10.1038/35888. PMID 9486653. Bibcode: 1998Natur.391..806F. 
  6. ↑ "The RNA-induced silencing complex: a versatile gene-silencing machine". The Journal of Biological Chemistry 284 (27): 17897–901. July 2009. doi:10.1074/jbc.R900012200. PMID 19342379. 
  7. ↑ "Functional genomic analysis of C. elegans chromosome I by systematic RNA interference". Nature 408 (6810): 325–30. November 2000. doi:10.1038/35042517. PMID 11099033. Bibcode: 2000Natur.408..325F. 
  8. ↑ "RNAi therapeutics: principles, prospects and challenges". Advanced Drug Delivery Reviews 59 (2–3): 75–86. March 2007. doi:10.1016/j.addr.2007.03.005. PMID 17449137. 
  9. ↑ "Genome-wide RNAi screening in Caenorhabditis elegans". Methods 30 (4): 313–21. August 2003. doi:10.1016/S1046-2023(03)00050-1. PMID 12828945. 
  10. ↑ "Small Players Ruling the Hard Game: siRNA in Bone Regeneration". Journal of Bone and Mineral Research 31 (3): 475–87. March 2016. doi:10.1002/jbmr.2816. PMID 26890411. 
  11. ↑ "CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes". Cell 154 (2): 442–51. July 2013. doi:10.1016/j.cell.2013.06.044. PMID 23849981. 
  12. ↑ "Orthogonal Cas9 proteins for RNA-guided gene regulation and editing". Nature Methods 10 (11): 1116–21. November 2013. doi:10.1038/nmeth.2681. PMID 24076762. PMC 3844869. https://dash.harvard.edu/bitstream/handle/1/12406999/3844869.pdf?sequence=1. 
  13. ↑ "RNA-guided editing of bacterial genomes using CRISPR-Cas systems". Nature Biotechnology 31 (3): 233–9. March 2013. doi:10.1038/nbt.2508. PMID 23360965. 
  14. ↑ "Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination". Nucleic Acids Research 41 (20): e193. November 2013. doi:10.1093/nar/gkt805. PMID 24013562. 
  15. ↑ "Genome editing using artificial site-specific nucleases in zebrafish". Development, Growth & Differentiation 56 (1): 26–33. January 2014. doi:10.1111/dgd.12094. PMID 24117409. 
  16. ↑ "CRISPR/Cas-induced double-strand breaks boost the frequency of gene replacements for humanizing the mouse Cnr2 gene". Biochemical and Biophysical Research Communications 441 (4): 815–9. November 2013. doi:10.1016/j.bbrc.2013.10.138. PMID 24211574. 
  17. ↑ "Transcription activator-like effector nucleases (TALENs): a highly efficient and versatile tool for genome editing". Biotechnology and Bioengineering 110 (7): 1811–21. July 2013. doi:10.1002/bit.24890. PMID 23508559. 
  18. ↑ "Adenoviral Gene Knockdown Cells". Sirion Biotech. http://www.sirion-biotech.com/adenoviral_gene_knockdown.html. 

External links



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