Biology:P35 holin family
The PRD1 Phage P35 Holin (P35 Holin) Family (TC# 1.E.5) is a member of Holin Superfamily III.[1] The prototype for this family is the lipid-containing PRD1 enterobacterial phage holin protein P35 (12.8 kDa; TC# 1.E.5.1.1) encoded by gene XXXV (orfT).[2] It is a component of a typical holin-endolysin system which functions to lyse the host bacterial cell.
Structure
P35 holin (TC# 1.E.5.1.1) has 3 transmembrane segments (TMSs) with 5 positively charged residues between TMSs 1 and 2. It has 4 positively charged residues at the C-terminus.[2] It is therefore thought that the N-terminus is in the periplasm and the C-terminus is in the cytoplasm. Homologues of 109 amino acyl residues (aas), which also have 3 putative TMSs, are encoded in the genomes of Xylella fastidiosa strains.[2]
Function
The reaction catalyzed by P35 holin is:
- autolysin (in) → autolysin (out)
See also
References
- ↑ Reddy, Bhaskara L.; Saier, Milton H. (2013-11-01). "Topological and phylogenetic analyses of bacterial holin families and superfamilies". Biochimica et Biophysica Acta (BBA) - Biomembranes 1828 (11): 2654–2671. doi:10.1016/j.bbamem.2013.07.004. ISSN 0006-3002. PMID 23856191.
- ↑ 2.0 2.1 2.2 Rydman, Pia S.; Bamford, Dennis H. (2003-07-01). "Identification and mutational analysis of bacteriophage PRD1 holin protein P35". Journal of Bacteriology 185 (13): 3795–3803. doi:10.1128/jb.185.13.3795-3803.2003. ISSN 0021-9193. PMID 12813073.
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