View source for Biology:Dechloromonas agitata strain CKB

{{Taxobox
| domain = [[Biology:Bacteria|Bacteria]]
| phylum = [[Biology:Pseudomonadota|Pseudomonadota]]
| classis = [[Biology:Betaproteobacteria|Betaproteobacteria]]
| ordo = [[Biology:Rhodocyclales|Rhodocyclales]]
| familia = [[Biology:Rhodocyclaceae|Rhodocyclaceae]]
| genus = ''[[Biology:Dechloromonas|Dechloromonas]]''
| species = '''''D. agitata'''''
| binomial = ''Dechloromonas agitata''
| binomial_authority = 
| synonyms = 
}}

'''''Dechloromonas agitata'' strain CKB''' is a dissimilatory [[Chemistry:Perchlorate|perchlorate]] reducing [[Biology:Bacterium|bacterium]] (DRPB) that was isolated from paper mill waste. Strain CKB is a Gram negative, facultative anaerobe belonging to the [[Biology:Betaproteobacteria|Betaproteobacteria]]. The [[Biology:Cell|cell]]s of strain CKB are highly motile and possess a single polar [[Biology:Flagellum|flagellum]]. ''D. agitata'' can couple the oxidation of several [[Chemistry:Electron donor|electron donor]]s such as [[Chemistry:Acetate|acetate]], [[Chemistry:Propionate|propionate]], [[Chemistry:Butyrate|butyrate]], [[Chemistry:Lactic acid|lactate]], succinate, fumarate, malate or [[Biology:Yeast extract|yeast extract]] to electron acceptors such as [[Chemistry:Oxygen|oxygen]], [[Chemistry:Chlorate|chlorate]], [[Chemistry:Perchlorate|perchlorate]], ferrous iron, [[Chemistry:Sulfide|sulfide]], and reduced humic substances like 2,6-anthrahydroquinone disulfonate. Unlike other perchlorate reducers, strain CKB cannot grow by nitrate reduction, which suggests that the pathways of nitrate and perchlorate reduction are distinct and unrelated, contrary to what previous research had shown.

==Biological perchlorate reduction by strain CKB==
Strain CKB can reduce chlorate and perchlorate (collectively known as (per)chlorate) completely to [[Chemistry:Chloride|chloride]]. The first step of the pathway involves the reduction of (per)chlorate to [[Chemistry:Chlorite|chlorite]] via the [[Biology:Perchlorate reductase|perchlorate reductase]] (Pcr) [[Biology:Enzyme|enzyme]]. Chlorite is then dismutated to molecular oxygen and chloride by the [[Biology:Chlorite dismutase|chlorite dismutase]] (Cld) enzyme. The produced oxygen can then be reduced to water by action of the enzyme cytochrome oxidase.
The Cld enzyme, encoded by an 834 bp [[Biology:Gene|gene]], is 277 amino acids long and includes a 26 amino acid leader peptide. Though expressed at basal levels under aerobic conditions, the cld gene was transcriptionally upregulated when strain CKB cells were grown in (per)chlorate. The Cld enzyme is of particular interest not only because it protects cells from toxic chlorite, but also because it is one of only a handful of systems capable of producing molecular oxygen.

==References==
*{{Cite journal|title= Reduction of (per)chlorate by a novel organism isolated from paper mill waste|author1=Royce A. Bruce |author2=Laurie A. Achenbach |author3=John D. Coates | journal= Environmental Microbiology| volume= 1|issue=4 | year= 1999| pages= 319–329|pmid = 11207750|doi=10.1046/j.1462-2920.1999.00042.x}}
*{{Cite journal|title= Sequencing and Transcriptional Analysis of the Chlorite Dismutase Gene of Dechloromonas agitata and Its Use as a Metabolic Probe|author1=Kelly S. Bender |author2=Susan M. O’Connor |author3=Romy Chakraborty |author4=John D. Coates |author5=Laurie A. Achenbach | journal= Applied and Environmental Microbiology| volume= 68|issue=10 | year= 2002| pages= 4820–4826| doi = 10.1128/AEM.68.10.4820-4826.2002 | pmid = 12324326  | pmc= 126438}}

==External links==
*[http://bacdive.dsmz.de/index.php?search=13894&submit=Search Type strain of ''Dechloromonas agitata'' at Bac''Dive'' -  the Bacterial Diversity Metadatabase]

{{DISPLAYTITLE: ''Dechloromonas agitata'' strain CKB }}
{{Taxonbar|from=Q5249130}}

[[Category:Rhodocyclaceae]]



{{Sourceattribution|Dechloromonas agitata strain CKB}}