View source for Biology:Infectious salmon anemia virus

{{Taxobox
| name               = Infectious salmon anemia
| virus_group        = v
| familia            = [[Biology:Orthomyxoviridae|Orthomyxoviridae]]
| genus              = '''''Isavirus'''''
| species            = ''Infectious salmon anemia virus''
| subdivision_ranks  = Genera
| subdivision        = 
* ''Influenzavirus A''
* ''[[Biology:Influenzavirus B|Influenzavirus B]]''
* ''[[Biology:Influenzavirus C|Influenzavirus C]]''
* ''[[Biology:Influenzavirus D|Influenzavirus D]]''
* ''Isavirus''
* ''[[Biology:Quaranjavirus|Quaranjavirus]]''
* ''[[Biology:Thogotovirus|Thogotovirus]]
}}

'''Infectious salmon anemia''' ('''ISA''') is a [[Biology:Virus|viral]] [[Disease|disease]] of [[Biology:Atlantic salmon|Atlantic salmon]] (''Salmo salar'') that affects fish farms in {{wipe|Canada}}, {{wipe|Norway}}, Scotland and {{wipe|Chile}}, causing severe losses to infected farms. ISA has been a [[Organization:World Organisation for Animal Health|World Organisation for Animal Health]] notifiable disease since 1990.<ref>{{cite book|last1=Kibenge|first1=F.S.B.|last2=Kibenge|first2=M.J.T.|editor1-last=Kibenge|editor1-first=Frederick S. B.|editor2-last=Godoy|editor2-first=Marcos|title=Aquaculture Virology|date=2016|publisher=Academic Press|isbn=9780128017548|pages=305–315|chapter=19.2 Orthomyxoviruses of fish. Genus Isavirus}}</ref> In the [[Social:European Union|EU]], it is classified as a non-exotic disease, and is monitored by the European Community Reference Laboratory for Fish Diseases.

==Virology==
ISA is caused by the infectious salmon anemia virus (ISAV). ISAV, a RNA virus, is the only species in the genus "Isavirus" which is in the family [[Biology:Orthomyxoviridae|Orthomyxoviridae]] ,and therefore related to the [[Medicine:Influenza|influenza]] viruses.

The genome encodes at least 10 proteins.<ref name=Tello2013>{{cite journal|last1=Tello|first1=M|last2=Vergara|first2=F|last3=Spencer|first3=E|title=Genomic adaptation of the ISA virus to ''Salmo salar'' codon usage|journal=Virology Journal|date=5 July 2013|volume=10|pages=223|doi=10.1186/1743-422X-10-223|pmid=23829271|pmc=3706250}}</ref>

There are several distinct strains of the virus. The most common are a European strain and a North American strain.<ref name="CFSPH 2010">{{cite web|title=Infectious Salmon Anemia|url=http://www.cfsph.iastate.edu/Factsheets/pdfs/infectious_salmon_anemia.pdf|publisher=The Center for Food Security & Public Health. Institute for International Cooperation in Animal Biologics. Iowa State University|accessdate=2017-11-13|date=March 2010}}</ref>

== Pathology ==
ISA virus causes severe [[Medicine:Anemia|anemia]] in infected [[Biology:Fish|fish]]. Unlike the mature red blood cells of mammals, the mature [[Biology:Red blood cell|red blood cell]]s of fish contain DNA, and can become infected by viruses.<ref>{{cite book|last1=Roossinck|first1=Marilyn J.|title=Virus: An Illustrated Guide to 101 Incredible Microbes|publisher=Princeton University Press|isbn=978-0-691-16696-4|pages=116–117|chapter=Infectious salmon anemia virus}}</ref> The fish develop pale [[Biology:Gill|gill]]s, and may swim close to the water surface, gulping for air. However, the fish may show no external signs of illness and maintain a normal appetite, until suddenly dying. The disease can progress slowly throughout an infected farm and, in the worst cases, death rates may approach 100%. Post-mortem examination of the fish has shown a wide range of causes of death. The [[Biology:Liver|liver]] and [[Biology:Spleen|spleen]] may be swollen, congested or partially already dead. The [[Biology:Circulatory system|circulatory system]] may stop working, and the blood may be contaminated with dead [[Biology:Blood cell|blood cell]]s. Red blood cells still present burst easily, and the numbers of immature and damaged blood cells are increased.

Infectious salmon anemia appears to be most like [[Medicine:Influenza|influenza]] viruses. Its mode of transfer and the natural reservoirs of infectious salmon anemia virus are not fully understood. Apart from Atlantic salmon, both sea-run [[Biology:Brown trout|Brown trout]] (''Salmo trutta'') and [[Biology:Rainbow trout|Rainbow trout]] (''Onchorhyncus mykiss'') can be infected, but do not become sick, so it is thought possible that these species may act as important [[Medicine:Asymptomatic carrier|carriers]] and reservoirs of the virus.

Research shows that while several species of Pacific salmon can be carriers of the virus, even highly pathogenic strains, Pacific salmon currently show high relative resistance and no ISAV-related symptoms. However, the potential for ISAV adaptation to Pacific salmon exists.<ref>{{cite journal|last1=Rolland|first1=JB|last2=Winton|first2=JR|title=Relative resistance of Pacific salmon to infectious salmon anaemia virus.|journal=Journal of Fish Diseases|date=September 2003|volume=26|issue=9|pages=511–20|doi=10.1046/j.1365-2761.2003.00473.x|pmid=14575369|url=https://www.sfu.ca/content/dam/sfu/coastal/Research%20&%20Series/Series/Speaking%20for%20the%20Salmon/PathogensandDisease2011/RollandRelativeResistance.pdf|accessdate=2017-11-13}}</ref><ref>{{cite journal|last1=Kibenge|first1=FS|last2=Kibenge|first2=MJ|last3=Groman|first3=D|last4=McGeachy|first4=S|title=''In vivo'' correlates of infectious salmon anemia virus pathogenesis in fish|journal=The Journal of General Virology|date=September 2006|volume=87|issue=Pt 9|pages=2645–52|doi=10.1099/vir.0.81719-0|pmid=16894204}}{{open access}}</ref>

== Epidemiology ==

In the autumn of 1984, a new disease was observed in Atlantic salmon being farmed along the southwest coast of Norway. The disease, which was named infectious salmon anemia, spread slowly, but caused the death of up to 80% of salmon stock in some hatcheries.<ref>Collins Edexcel International GCSE Biology, Student Book ({{ISBN|978-0-00-745000-8}}) p. 328</ref> By June 1988 it had become sufficiently widespread and serious to require the Norwegian Ministry of Agriculture, Fisheries and Food to declare it a notifiable disease.

In the summer of 1996, a new disease appeared in Atlantic salmon being farmed in New Brunswick, Canada. The death rate of the fish on affected farms was very high and, following extensive scientific examination of the victims, the disease was named "hemorrhagic kidney syndrome." Although the source and distribution of this disease was not known, the results of studies by Norwegian and Canadian scientists showed conclusively that the same virus was responsible for both infectious salmon anemia and hemorrhagic kidney syndrome.

In May 1998, a salmon farm at Loch Nevis on the west coast of Scotland reported its suspicions of an outbreak of infectious salmon anemia. The suspicions were confirmed, and by the end of the year, the disease had spread to an additional fifteen farms not only on the Scottish mainland but also on Skye and Shetland.

In 2008, there was an outbreak of ISA in Shetland.<ref>{{cite web|url=http://www.fis.com/fis/worldnews/worldnews.aspfil=e&ndb=1&id=31184|publisher=Fish Information & Services}}{{dead link|date=January 2018 |bot=InternetArchiveBot |fix-attempted=yes }}</ref> ISA was detected in fish from just one site. There is no evidence the disease has spread beyond this site, but two nearby SSF cages are under suspicion of carrying the disease and are also now clear of fish.

In 2011, two wild Pacific salmon taken from the central coast of British Columbia were suspected to have ISA after preliminary tests showed possible evidence of the virus. However, extensive testing by the Canadian Food Inspection Agency to try to amplify and culture the virus were unsuccessful, prompting the agency to conclude that the ISA virus was not present.<ref>{{cite web|url=http://www.inspection.gc.ca/english/corpaffr/newcom/2011/20111109e.shtml|publisher=Canadian Food Inspection Agency}}</ref> In February 2012, a confirmed outbreak of ISA in Nova Scotia resulted in the destruction of up to 750,000 salmon.{{citation needed|date=January 2013}} In July 2012, a confirmed outbreak of infectious salmon anemia in Newfoundland and Labrador, Canada, prompted the destruction of 450,000 farmed salmon by the Canadian Food Inspection Agency<ref>{{cite news|url=https://www.theglobeandmail.com/news/toronto/450000-salmon-to-be-destroyed-after-outbreak-of-anemia-in-newfoundland-and-labrador/article4398076/|title=450,000 salmon to be destroyed after outbreak in Newfoundland and Labrador|publisher=The Globe and Mail|date=7 July 2012|accessdate=24 January 2013}}</ref> and an outbreak was also confirmed at another site in late 2012.{{citation needed|date=January 2013}} In mid-2012, another outbreak was identified in Nova Scotia, with the 240,000 fish being allowed by CFIA to mature to market size before being harvested in early 2013 by the operator and processed for the consumer market. After being held by the CFIA, the fish was declared fit for human consumption despite the presence of the virus as the disease "poses no risk to human health".<ref>{{cite news|url=https://www.thestar.com/news/canada/2013/02/01/infected_salmon_declared_fit_for_human_consumption_by_canadian_food_inspection_agency.html|title=Infected salmon declared fit for human consumption by Canadian Food Inspection Agency|publisher=The Toronto Star|date=1 February 2013|accessdate=1 February 2013}}</ref> In January 2016, it was announced that the virus had been discovered in farmed and wild salmon British Columbia for the first time.<ref>{{cite journal|last1=Kibenge|first1=Molly JT|last2=Iwamoto|first2=Tokinori|last3=Wang|first3=Yingwei|last4=Morton|first4=Alexandra|last5=Routledge|first5=Richard|last6=Kibenge|first6=Frederick SB|title=Discovery of variant infectious salmon anaemia virus (ISAV) of European genotype in British Columbia, Canada|journal=Virology Journal|volume=13|issue=1|year=2016|issn=1743-422X|doi=10.1186/s12985-015-0459-1}}</ref>

In {{wipe|Chile}}, ISA was first isolated from a salmon farm in the 1990s and described for the first time in 2001,<ref>{{cite journal |author1=Kibenge F.S.B |author2=Garate O.N |author3=Johnson G |author4=Arriagada R |author5=Kibenge M.J.T |author6=Wadowska D |title=Isolation and identification of infectious salmon anaemia virus (ISAV) from Coho salmon in Chile |journal=Dis. Aquat. Org. |volume=45 |pages=9–18 |year=2001 |doi=10.3354/dao045009 |pmid=11411649 |issue=1 }}</ref> although the initial presence never resulted in widespread problems. However, since June 2007, the national industry has been dealing with a serious ISA outbreak which has not yet been completely brought under control and has been responsible for an important decline in the industry, closure of many farms and high unemployment. The virus was detected in an Atlantic salmon farm in Chiloé Archipelago in [[Place:Los Lagos Region|Los Lagos Region]] and spread to the fjords and channels of [[Place:Aysén Region|Aysén Region]] to the south almost immediately.<ref>{{cite web|title=Analizan posible presencia de variante de virus ISA|url=http://www.aqua.cl/noticias/index.phpfidoc=18919|language=es}}{{Dead link|date=September 2018 |bot=InternetArchiveBot |fix-attempted=yes }}</ref><ref>{{cite journal|last1=Godoy|first1=MG|last2=Aedo|first2=A|last3=Kibenge|first3=MJ|last4=Groman|first4=DB|last5=Yason|first5=CV|last6=Grothusen|first6=H|last7=Lisperguer|first7=A|last8=Calbucura|first8=M|last9=Avendaño|first9=F|last10=Imilán|first10=M|last11=Jarpa|first11=M|last12=Kibenge|first12=FS|title=First detection, isolation and molecular characterization of infectious salmon anaemia virus associated with clinical disease in farmed Atlantic salmon (''Salmo salar'') in Chile|journal=BMC Veterinary Research|date=4 August 2008|volume=4|pages=28|doi=10.1186/1746-6148-4-28|pmid=18680586|pmc=2519066}}</ref>

== Transmission ==
The virus is spread by contact with infected fish or their secretions, or contact with equipment or people who have handled infected fish. The virus can survive in seawater, so a major risk factor for any uninfected farm is its proximity to an already infected farm.

The ''Lepeophtheirus salmonis'' [[Biology:Sea louse|sea louse]], a small [[Biology:Crustacean|crustacean]] [[Biology:Parasite|parasite]] that attacks the protective [[Biology:Mucus|mucus]], [[Biology:Scale (zoology)|scales]] and [[Biology:Skin|skin]] of the salmon, can carry the virus passively on its surface and in its digestive tract. Under laboratory conditions ''Lepeophtheirus salmonis'' can passively transfer ISA.<ref>{{cite web|title=OIE ISA fact sheet|url=http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/2010/2.3.05_ISA%20.pdf|deadurl=yes|archiveurl=https://web.archive.org/web/20111111235349/http://www.oie.int/fileadmin/Home/eng/Health_standards/aahm/2010/2.3.05_ISA%20.pdf|archivedate=2011-11-11|df=}}</ref> It is not known whether the ISA virus can reproduce itself in the sea louse, although this is a possibility as viruses can use secondary vectors for transmission, for example Arboviruses such as [[Medicine:Dengue fever|dengue fever]], West Nile virus, or [[Biology:African swine fever virus|African swine fever virus]].

==Diagnosis==
Clinical signs and pathology may suggest infection. Viral identification is possible using [[Biology:Immunofluorescence|immunofluorescence]] and PCR.<ref name="WikiVet">[http://en.wikivet.net/Infectious_Salmon_Anaemia Infectious Salmon Anaemia] expert reviewed and published by WikiVet, accessed 10 October 2011.</ref>

== Treatment and control ==
There is no treatment once fish are infected.<ref name="WikiVet"/>

ISA is a major threat to the viability of salmon farming and is now the first of the diseases classified on List One of the European Commission’s fish health regime. Amongst other measures, this requires the total eradication of the entire fish stock should an outbreak of the disease be confirmed on any farm. The economic and social consequences of both the disease and the measures used to control it are thus very far reaching.

Infectious salmon anemia is currently regarded as a serious threat not only to farmed Atlantic salmon, but also to dwindling stocks of wild Atlantic salmon. Recent research involving a multi-year study of wild Atlantic salmon from North America shows that infected salmon that survive infection generate antibodies against the virus.<ref>{{cite journal|last1=Cipriano|first1=R. C.|title=Antibody against infectious salmon anaemia virus among feral Atlantic salmon (''Salmo salar'')|journal=ICES Journal of Marine Science|date=18 May 2009|volume=66|issue=5|pages=865–870|doi=10.1093/icesjms/fsp124}}{{open access}}</ref> Work is now underway to develop a [[Biology:Vaccine|vaccine]] against ISA.

==Evolution==
There are several distinct strains of the virus, some are pathogenic and some are not. The most common are a European strain and a North American strain.<ref name="CFSPH 2010"/>

Genetic research into the ISA virus shows that the European and North American strains of the virus diverged from each other sometime around 1900.<ref>{{cite journal|last1=Krossøy|first1=B|last2=Nilsen|first2=F|last3=Falk|first3=K|last4=Endresen|first4=C|last5=Nylund|first5=A|title=Phylogenetic analysis of infectious salmon anaemia virus isolates from Norway, Canada and Scotland.|journal=Diseases of Aquatic Organisms|date=26 January 2001|volume=44|issue=1|pages=1–6|pmid=11253869|url=http://www.ottokinne.de/articles/dao/44/d044p001.pdf|archive-url=https://web.archive.org/web/20120426042016/http://www.ottokinne.de/articles/dao/44/d044p001.pdf|dead-url=yes|archive-date=26 April 2012|doi=10.3354/dao044001}}</ref> This research points out that starting in 1879 when rainbow trout were first brought to Europe from North America, there were many transfers of fish across the Atlantic ocean which may have carried the ISA virus. Some species were introduced to Europe from North America, and some species were introduced to North America from Europe. Given that the virus did not evolve into two separate strains until around 1900, and given how many transfers of fish there were, according to this research, "At present it is therefore not possible to suggest a geographical origin of the ISA virus."

Another study suggests that this virus was introduced into {{wipe|Norway}} between 1932 and 1959 and that the original strain was the European subtype found in North America.<ref name=Plarre2012>{{cite journal|last1=Plarre|first1=H|last2=Nylund|first2=A|last3=Karlsen|first3=M|last4=Brevik|first4=Ø|last5=Sæther|first5=PA|last6=Vike|first6=S|title=Evolution of infectious salmon anaemia virus (ISA virus)|journal=Archives of Virology|date=December 2012|volume=157|issue=12|pages=2309–26|doi=10.1007/s00705-012-1438-0|pmid=22886279}}</ref> The strains found in {{wipe|Chile}} were transmitted from Norway between 1995 and 2007.

== References ==
{{Reflist}}

=== Bibliography ===
{{Refbegin}}
* {{cite web|last1=Ely|first1=Barry|title=Mill Hill Essays: Infectious Salmon Anaemia|url=http://www.historyofnimr.org.uk/mill-hill-essays/essays-yearly-volumes/1999-2/infectious-salmon-anaemia/|website=NIMR History|date=1999}}
* {{cite journal |vauthors=Raynard RS, Murray AG, Gregory A |title=Infectious salmon anaemia virus in wild fish from Scotland |journal=Diseases of Aquatic Organisms |volume=46 |pages=93–100 |year=2001 |doi=10.3354/dao046093 |pmid=11678233 |issue=2 }}
{{Refend}}

==External links==
* [http://www.expasy.org/viralzone/all_by_species/95.html '''Viralzone''': Isavirus]

{{fish disease topics}}
{{Taxonbar|from=Q547421}}

{{DEFAULTSORT:Infectious Salmon Anemia Virus}}
[[Category:Orthomyxoviridae]]
[[Category:Salmonidae]]

{{Sourceattribution|Infectious salmon anemia virus|1}}