View source for Biology:L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

{{Short description|Class of enzymes}}
{{Infobox enzyme
| Name       = L-cysteine:1D-''myo''-inositol 2-amino-2-deoxy-α-D-glucopyranoside ligase
| EC_number  = 6.3.1.13
| CAS_number =
| GO_code    = 
| image      = 
| width      = 
| caption    =  
}}
'''L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase''' ({{EnzExplorer|6.3.1.13}}, ''MshC'', ''MshC ligase'', ''Cys:GlcN-Ins ligase'', ''mycothiol ligase'') is an [[Biology:Enzyme|enzyme]] with [[Biology:List of enzymes|systematic name]] ''L-cysteine:1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol ligase (AMP-forming)''.<ref>{{cite journal | vauthors = Fan F, Luxenburger A, Painter GF, Blanchard JS | title = Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC) | journal = Biochemistry | volume = 46 | issue = 40 | pages = 11421–9 | date = October 2007 | pmid = 17848100 | pmc = 2526253 | doi = 10.1021/bi7011492 }}</ref><ref>{{cite journal | vauthors = Gutierrez-Lugo MT, Newton GL, Fahey RC, Bewley CA | title = Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis | journal = Protein Expression and Purification | volume = 50 | issue = 1 | pages = 128–36 | date = November 2006 | pmid = 16908186 | doi = 10.1016/j.pep.2006.07.005 | url = https://zenodo.org/record/1259301/files/article.pdf }}</ref><ref>{{cite journal | vauthors = Tremblay LW, Fan F, Vetting MW, Blanchard JS | title = The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway | journal = Biochemistry | volume = 47 | issue = 50 | pages = 13326–35 | date = December 2008 | pmid = 19053270 | pmc = 2628295 | doi = 10.1021/bi801708f }}</ref> This enzyme [[Chemistry:Catalysis|catalyses]] the following [[Chemistry:Chemical reaction|chemical reaction]]

: 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-[[Chemistry:Cysteine|cysteine]] + ATP <math>\rightleftharpoons</math> 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate

This enzyme is a key enzyme in the biosynthesis of [[Chemistry:Mycothiol|mycothiol]].

== References ==
{{reflist}}

== External links ==
* {{MeshName|L-cysteine:1D-myo-inositol+2-amino-2-deoxy-alpha-D-glucopyranoside+ligase}}

{{Ligases CO CS and CN}}
{{Enzymes}}

[[Category:EC 6.3.1]]

{{Sourceattribution|L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase}}