View source for Biology:TBX2

{{Short description|Protein-coding gene in the species Homo sapiens}}
{{Infobox_gene}}
'''T-box transcription factor 2 Tbx2''' is a [[Biology:Transcription factor|transcription factor]] that is encoded by the ''Tbx2'' [[Biology:Gene|gene]] on chromosome 17q21-22 in humans.<ref name="pmid8530034">{{cite journal | vauthors = Campbell C, Goodrich K, Casey G, Beatty B | title = Cloning and mapping of a human gene (TBX2) sharing a highly conserved protein motif with the Drosophila omb gene | journal = Genomics | volume = 28 | issue = 2 | pages = 255–260 | date = July 1995 | pmid = 8530034 | doi = 10.1006/geno.1995.1139 }}</ref><ref name="entrez">{{EntrezGene|6909}}</ref><ref>{{cite journal | vauthors = Law DJ, Gebuhr T, Garvey N, Agulnik SI, Silver LM | title = Identification, characterization, and localization to chromosome 17q21-22 of the human TBX2 homolog, member of a conserved developmental gene family | journal = Mammalian Genome | volume = 6 | issue = 11 | pages = 793–797 | date = November 1995 | pmid = 8597636 | doi = 10.1007/bf00539006 | hdl-access = free | s2cid = 15779406 | hdl = 2027.42/47017 }}</ref>  This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the [[Biology:T-box|T-box]].  Tbx2 and [[Biology:TBX3|Tbx3]] are the only T-box transcription factors that act as transcriptional repressors rather than transcriptional activators, and are closely related in terms of development and tumorigenesis.<ref name="Lu_2010">{{cite journal | vauthors = Lu J, Li XP, Dong Q, Kung HF, He ML | title = TBX2 and TBX3: the special value for anticancer drug targets | journal = Biochimica et Biophysica Acta (BBA) - Reviews on Cancer | volume = 1806 | issue = 2 | pages = 268–274 | date = December 2010 | pmid = 20624445 | pmc = 7127380 | doi = 10.1016/j.bbcan.2010.07.001 }}</ref>  This gene plays a significant role in embryonic and fetal development through control of gene expression, and also has implications in various cancers.  Tbx2 is associated with numerous signaling pathways, [[Biology:Bone morphogenetic protein|BMP]], TGFβ, [[Biology:Wnt signaling pathway|Wnt]], and FGF, which allow for patterning and proliferation during organogenesis in fetal development.<ref name="Lu_2010" />

== Role in development ==
The molecule Tbx-2 is a transcription factor in the T box transcription factor family. Tbx2 helps form the outflow tract and atrioventricular canal. Tbx2 can repress genes as well as being competitors that take over binding sites. It also plays a role in cancer because it will suppress cell growth and supports invasiveness. In human melanoma, the expression of endogenous Tbx 2 is shown to help reduce the growth of melanomas. It has also been shown that overexpression of Tbx2 can lead to breast cancer. Tbx2 has shown septal defects of the outflow tract, and this has been shown using a knockout mouse. The knockout mouse is a mouse in which the gene is inactivated in order to study the role of genes. Tbx 2 also helps in regulating the cell cycle. This was first shown when Tbx2 was found in a chromosomal region that is often mutated in ovarian cancer and pancreatic cancer cells.<ref>{{cite journal | vauthors = Vance KW, Carreira S, Brosch G, Goding CR | title = Tbx2 is overexpressed and plays an important role in maintaining proliferation and suppression of senescence in melanomas | journal = Cancer Research | volume = 65 | issue = 6 | pages = 2260–2268 | date = March 2005 | pmid = 15781639 | doi = 10.1158/0008-5472.CAN-04-3045 | doi-access = free }}</ref>

During fetal development, the relationship of Tbx2 to FGF, BMP, and Wnt signaling pathways indicates its extensive control in development of various organ systems.  It functions predominantly in the patterning of organ development rather than tissue proliferation. Tbx2 has implications in limb development, atrioventricular development of the heart, and development of the anterior brain tissues.<ref name="Farin_2013">{{cite journal | vauthors = Farin HF, Lüdtke TH, Schmidt MK, Placzko S, Schuster-Gossler K, Petry M, Christoffels VM, Kispert A | display-authors = 6 | title = Tbx2 terminates shh/fgf signaling in the developing mouse limb bud by direct repression of gremlin1 | journal = PLOS Genetics | volume = 9 | issue = 4 | pages = e1003467 | date = 2013-04-25 | pmid = 23633963 | pmc = 3636256 | doi = 10.1371/journal.pgen.1003467 | doi-access = free }}</ref><ref name="Singh_2012">{{cite journal | vauthors = Singh R, Hoogaars WM, Barnett P, Grieskamp T, Rana MS, Buermans H, Farin HF, Petry M, Heallen T, Martin JF, Moorman AF, 't Hoen PA, Kispert A, Christoffels VM | display-authors = 6 | title = Tbx2 and Tbx3 induce atrioventricular myocardial development and endocardial cushion formation | journal = Cellular and Molecular Life Sciences | volume = 69 | issue = 8 | pages = 1377–1389 | date = April 2012 | pmid = 22130515 | pmc = 3314179 | doi = 10.1007/s00018-011-0884-2 }}</ref><ref>{{cite journal | vauthors = Cho GS, Park DS, Choi SC, Han JK | title = Tbx2 regulates anterior neural specification by repressing FGF signaling pathway | journal = Developmental Biology | volume = 421 | issue = 2 | pages = 183–193 | date = January 2017 | pmid = 27913219 | doi = 10.1016/j.ydbio.2016.11.020 | doi-access = free }}</ref>

During limb bud development, Shh and FGF signaling stimulate the outgrowth of the limb.  At a certain point, Tbx2 concentrations are such that the signaling of Shh and FGF are terminated, halting further progression and outgrowth of the limb development.  This occurs directly through Tbx2 repressing the expression of Grem1, creating a negative Grem1 zone, thereby disrupting the outgrowth signaling by Shh and FGF.<ref name="Farin_2013" />

Cardiac development is heavily regulated and requires the development of the four cardiac chambers, septum, and various valve components for outflow and inflow.  In heart development, Tbx2 is up-regulated by [[Biology:Bone morphogenetic protein 2|BMP2]] to stimulate atrioventricular development.<ref name="Singh_2012" />  The development of a Tbx2 knockout mouse model allowed for the determination of specific roles of Tbx2 in cardiac development, and scientists determined Tbx2 and Tbx3 to be redundant in much of heart development.<ref name="Singh_2012" />  Further, the use of these knockout models determined the significance of Tbx2 in the BMP signaling pathway for development of the atrioventricular canal, atrioventricular nodal phenotype, and atrioventricular cushion.<ref name="Singh_2012" />

The atrioventricular canal signaling cascade involves the atrial natriuretic factor gene (''ANF''). This gene is one of the first hallmarks of chamber formation in the developing myocardium. A small fragment within this gene can repress the promoter of cardiac troponin I (''cTnI'') selectively in the atrioventricular canal. T-box factor and NK2-homeobox factor binding element are involved in the repression of the atrioventricular canal without affecting its chamber activity. Tbx2 forms a complex with Nkx2.5 on the ANF gene to repress its promoter activity, so that the gene's expression is inhibited in the atrioventricular canal during chamber differentiation.<ref>{{cite journal | vauthors = Habets PE, Moorman AF, Clout DE, van Roon MA, Lingbeek M, van Lohuizen M, Campione M, Christoffels VM | display-authors = 6 | title = Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the atrioventricular canal: implications for cardiac chamber formation | journal = Genes & Development | volume = 16 | issue = 10 | pages = 1234–1246 | date = May 2002 | pmid = 12023302 | pmc = 186286 | doi = 10.1101/gad.222902 }}</ref> The atrioventricular canal is also the origin of the atrioventricular nodal axis and helps eventually coordinate the beating heart. The role of Tbx2 in cushion formation in the developing heart is by working with Tbx3 to trigger a feed-forward loop with BMP2 for the coordinated development of these cushions.<ref name="Singh_2012"/> Tbx2 has also been found to temporally suppress the proliferation and differentiation a subset of the primary myocardial cells.<ref>{{cite journal | vauthors = Aanhaanen WT, Brons JF, Domínguez JN, Rana MS, Norden J, Airik R, Wakker V, de Gier-de Vries C, Brown NA, Kispert A, Moorman AF, Christoffels VM | display-authors = 6 | title = The Tbx2+ primary myocardium of the atrioventricular canal forms the atrioventricular node and the base of the left ventricle | journal = Circulation Research | volume = 104 | issue = 11 | pages = 1267–1274 | date = June 2009 | pmid = 19423846 | doi = 10.1161/CIRCRESAHA.108.192450 | doi-access = free }}</ref>

Finally, during anterior brain development, BMP stimulates the expression of Tbx2, which suppresses FGF signaling.  This suppression of FGF signaling further represses the expression of [[Biology:FLRT3|Flrt3]], which is necessary for anterior brain development.

Tbx2 has been shown to be a [[Biology:Master regulator|master regulator]] in the differentiation of inner and outer [[Biology:Hair cell|hair cell]]s.<ref name="García-Añoveros 2022">{{cite journal | last1= García-Añoveros | first1=Jaime | last2=Clancy | first2=John C. | last3=Foo | first3=Chuan Zhi | last4=García-Gómez | first4=Ignacio | last5=Zhou | first5=Yingjie | last6=Homma | first6=Kazuaki | last7=Cheatham | first7=Mary Ann | last8=Duggan | first8=Anne | title=Tbx2 is a master regulator of inner versus outer hair cell differentiation | journal=Nature | date=2022-05-04 | volume=605 | issue=7909 | issn=1476-4687 | doi=10.1038/s41586-022-04668-3 |pmid=35508658 | pages=298–303 | pmc=9803360 | bibcode=2022Natur.605..298G | s2cid=248527577 }}</ref>

== Associated congenital defects ==

It is known that Tbx2 functions in a dose-dependent manner; therefore, duplication or deletion of the region encompassing Tbx2 can cause various congenital defects, including: [[Medicine:Microcephaly|microcephaly]], various ventricular-septal defects, and skeletal abnormalities.<ref>{{cite journal | vauthors = Pang S, Liu Y, Zhao Z, Huang W, Chen D, Yan B | title = Novel and functional sequence variants within the TBX2 gene promoter in ventricular septal defects | journal = Biochimie | volume = 95 | issue = 9 | pages = 1807–1809 | date = September 2013 | pmid = 23727221 | doi = 10.1016/j.biochi.2013.05.007 }}</ref><ref name = "Nimmakayalu_2011">{{cite journal | vauthors = Nimmakayalu M, Major H, Sheffield V, Solomon DH, Smith RJ, Patil SR, Shchelochkov OA | title = Microdeletion of 17q22q23.2 encompassing TBX2 and TBX4 in a patient with congenital microcephaly, thyroid duct cyst, sensorineural hearing loss, and pulmonary hypertension | journal = American Journal of Medical Genetics. Part A | volume = 155A | issue = 2 | pages = 418–423 | date = February 2011 | pmid = 21271665 | doi = 10.1002/ajmg.a.33827 | s2cid = 24377700 }}</ref><ref name = "Radio_2010">{{cite journal | vauthors = Radio FC, Bernardini L, Loddo S, Bottillo I, Novelli A, Mingarelli R, Dallapiccola B | title = TBX2 gene duplication associated with complex heart defect and skeletal malformations | journal = American Journal of Medical Genetics. Part A | volume = 152A | issue = 8 | pages = 2061–2066 | date = August 2010 | pmid = 20635360 | doi = 10.1002/ajmg.a.33506 | s2cid = 13614834 }}</ref>  Some specific abnormalities are discussed further below. Mutations in TBX2 cause predisposition to hernias.<ref>{{cite journal | vauthors = Zhang Y, Han Q, Fan H, Li W, Xing Q, Yan B | title = Genetic analysis of the TBX2 gene promoter in indirect inguinal hernia | journal = Hernia | volume = 18 | issue = 4 | pages = 513–517 | date = August 2014 | pmid = 24309999 | doi = 10.1007/s10029-013-1199-z | s2cid = 10028037 }}</ref>

=== Abnormalities of the digits ===

During limb bud development, down-regulation of Tbx2 fails to inhibit Shh/FGF4 signaling; therefore, resulting in increased limb bud size and duplication of the 4th digit, polydactyly.<ref name="Farin_2013" />  Opposite this, when Tbx2 is over expressed or duplicated, limb buds are smaller and can have reduced digit number because of the early termination of [[Biology:Sonic hedgehog|Shh]] and FGF4 signaling.<ref name="Farin_2013" />

=== Ventricular septal defects ===

This is a broad category encompassing many more specific congenital heart defects.  Of those related to Tbx2, some are caused by duplication, or over expression, of Tbx2, and others are caused by deletion of the Tbx2 gene region.  For example, patients with a duplication of the Tbx2 gene region have presented with atrioventricular abnormalities including: interventricular septal defect, patent foramen ovale, aortic coarctation, tricuspid valve insufficiency, and mitral valve stenosis.<ref name = "Radio_2010" />  Contrary, those with Tbx2 gene deletion have presented with pulmonary hypertension and other heart defects, but is less reported.<ref>{{cite journal | vauthors = Puusepp H, Zilina O, Teek R, Männik K, Parkel S, Kruustük K, Kuuse K, Kurg A, Ounap K | display-authors = 6 | title = 5.9 Mb microdeletion in chromosome band 17q22-q23.2 associated with tracheo-esophageal fistula and conductive hearing loss | journal = European Journal of Medical Genetics | volume = 52 | issue = 1 | pages = 71–74 | date = January 2009 | pmid = 18983945 | doi = 10.1016/j.ejmg.2008.09.006 }}</ref><ref name = "Nimmakayalu_2011" />

== Role in tumorigenesis ==

Tbx2 has been implicated in cancers associated with the lung, breast, bone, pancreas, and melanoma.  It is known to be over-expressed in this group of cancers, altering cell-signaling pathways leading to tumorigenesis.  Several pathways have been suggested and studied using mouse knockout models of genes within the signaling pathways.  Currently, research using the knockout model of Tbx2 for study of tumorigenesis is limited.

'''p14ARF/MDM2/p35/p21<sup>CIP1</sup> Pathway'''.  When up-regulated, Tbx2 inhibits [[Biology:P21|p21<sup>CIP1</sup>]]<sup>.</sup>  p21<sup>CIP1</sup> is necessary for tissue senescence, and when compromised, leaves the tissue vulnerable to tumor-promoting signals.<ref>{{cite journal | vauthors = Prince S, Carreira S, Vance KW, Abrahams A, Goding CR | title = Tbx2 directly represses the expression of the p21(WAF1) cyclin-dependent kinase inhibitor | journal = Cancer Research | volume = 64 | issue = 5 | pages = 1669–1674 | date = March 2004 | pmid = 14996726 | doi = 10.1158/0008-5472.can-03-3286 | doi-access = free }}</ref>

'''Wnt/beta-catenin Pathway'''.  The role of Tbx2 in Wnt signaling has yet to be confirmed; however, up-regulation of Tbx2 in the [[Biology:Beta-catenin|beta-catenin]] signaling pathway leads to loss of the adhesion molecule [[Biology:CDH1 (gene)|E-cadherin]].<ref>{{cite journal | vauthors = Rodriguez M, Aladowicz E, Lanfrancone L, Goding CR | title = Tbx3 represses E-cadherin expression and enhances melanoma invasiveness | journal = Cancer Research | volume = 68 | issue = 19 | pages = 7872–7881 | date = October 2008 | pmid = 18829543 | doi = 10.1158/0008-5472.can-08-0301 | doi-access = free }}</ref>  This returns cells to a mesenchymal state, and facilitates invasion of tumor cells.

'''EGR1 Signaling Pathway'''.  Finally, Tbx2 up-regulation increases its interaction with [[Biology:EGR1|EGR1]].  EGR1 represses [[Biology:NDRG1|NDGR1]] to increase cell proliferation, resulting in metastasis or tumor development.<ref>{{cite journal | vauthors = Redmond KL, Crawford NT, Farmer H, D'Costa ZC, O'Brien GJ, Buckley NE, Kennedy RD, Johnston PG, Harkin DP, Mullan PB | display-authors = 6 | title = T-box 2 represses NDRG1 through an EGR1-dependent mechanism to drive the proliferation of breast cancer cells | journal = Oncogene | volume = 29 | issue = 22 | pages = 3252–3262 | date = June 2010 | pmid = 20348948 | doi = 10.1038/onc.2010.84 | doi-access = free }}</ref>

Together, the up-regulation of Tbx2 on these signaling pathways can lead to development of malignant tumors.

== Cancer treatment target ==
Understanding the signaling pathways, and the role of Tbx2 in tumorigenesis, can aid in developing gene-targeted cancer treatments.  Because Tbx2 is up-regulated in various types of cancer cells in multiple organ systems, the potential for gene therapy is optimistic.  Scientists are interested in targeting a small domain of Tbx2 and Tbx3 to reduce its expression, and utilize small peptides known to suppress tumor genes to inhibit proliferation.  An in vitro study using a cell line of human prostate cancer blocked endogenous Tbx2 using Tbx2 dominant-negative retroviral vectors found reduced tumor cell proliferation.<ref>{{cite journal | vauthors = Nandana S, Tripathi M, Duan P, Chu CY, Mishra R, Liu C, Jin R, Yamashita H, Zayzafoon M, Bhowmick NA, Zhau HE, Matusik RJ, Chung LW | display-authors = 6 | title = Bone Metastasis of Prostate Cancer Can Be Therapeutically Targeted at the TBX2-WNT Signaling Axis | journal = Cancer Research | volume = 77 | issue = 6 | pages = 1331–1344 | date = March 2017 | pmid = 28108510 | pmc = 5783646 | doi = 10.1158/0008-5472.can-16-0497 }}</ref>  Further, the same study suggests targeting [[Biology:WNT3A|WNT3A]] because of its role in cell-signaling with Tbx2, by utilizing a WNT antagonist such as SFRP-2.  Because somatic cells have low expression of Tbx2, a targeted Tbx2 gene treatment would leave healthy somatic cells unharmed, thereby providing a treatment with low toxicity and negative side effects.<ref name="Lu_2010" />  Much research is still required to determine the efficacy of these specific gene targets to anti-cancer treatments.

== References ==
{{reflist|33em}}

== Further reading ==
{{refbegin|33em}}
* {{cite journal | vauthors = Bonaldo MF, Lennon G, Soares MB | title = Normalization and subtraction: two approaches to facilitate gene discovery | journal = Genome Research | volume = 6 | issue = 9 | pages = 791–806 | date = September 1996 | pmid = 8889548 | doi = 10.1101/gr.6.9.791 | doi-access = free }}
* {{cite journal | vauthors = Campbell CE, Casey G, Goodrich K | title = Genomic structure of TBX2 indicates conservation with distantly related T-box genes | journal = Mammalian Genome | volume = 9 | issue = 1 | pages = 70–73 | date = January 1998 | pmid = 9434949 | doi = 10.1007/s003359900682 | s2cid = 22349 }}
* {{cite journal | vauthors = Carreira S, Dexter TJ, Yavuzer U, Easty DJ, Goding CR | title = Brachyury-related transcription factor Tbx2 and repression of the melanocyte-specific TRP-1 promoter | journal = Molecular and Cellular Biology | volume = 18 | issue = 9 | pages = 5099–5108 | date = September 1998 | pmid = 9710594 | pmc = 109095 | doi = 10.1128/mcb.18.9.5099 }}
* {{cite journal | vauthors = He M, Wen L, Campbell CE, Wu JY, Rao Y | title = Transcription repression by Xenopus ET and its human ortholog TBX3, a gene involved in ulnar-mammary syndrome | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 96 | issue = 18 | pages = 10212–10217 | date = August 1999 | pmid = 10468588 | pmc = 17868 | doi = 10.1073/pnas.96.18.10212 | doi-access = free | bibcode = 1999PNAS...9610212H }}
* {{cite journal | vauthors = Sinha S, Abraham S, Gronostajski RM, Campbell CE | title = Differential DNA binding and transcription modulation by three T-box proteins, T, TBX1 and TBX2 | journal = Gene | volume = 258 | issue = 1–2 | pages = 15–29 | date = November 2000 | pmid = 11111039 | doi = 10.1016/S0378-1119(00)00417-0 }}
* {{cite journal | vauthors = Brummelkamp TR, Kortlever RM, Lingbeek M, Trettel F, MacDonald ME, van Lohuizen M, Bernards R | title = TBX-3, the gene mutated in Ulnar-Mammary Syndrome, is a negative regulator of p19ARF and inhibits senescence | journal = The Journal of Biological Chemistry | volume = 277 | issue = 8 | pages = 6567–6572 | date = February 2002 | pmid = 11748239 | doi = 10.1074/jbc.M110492200 | doi-access = free }}
* {{cite journal | vauthors = Lingbeek ME, Jacobs JJ, van Lohuizen M | title = The T-box repressors TBX2 and TBX3 specifically regulate the tumor suppressor gene p14ARF via a variant T-site in the initiator | journal = The Journal of Biological Chemistry | volume = 277 | issue = 29 | pages = 26120–26127 | date = July 2002 | pmid = 12000749 | doi = 10.1074/jbc.M200403200 | doi-access = free }}
* {{cite journal | vauthors = Habets PE, Moorman AF, Clout DE, van Roon MA, Lingbeek M, van Lohuizen M, Campione M, Christoffels VM | display-authors = 6 | title = Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the atrioventricular canal: implications for cardiac chamber formation | journal = Genes & Development | volume = 16 | issue = 10 | pages = 1234–1246 | date = May 2002 | pmid = 12023302 | pmc = 186286 | doi = 10.1101/gad.222902 }}
* {{cite journal | vauthors = Andelfinger G, Etter L, Dyment M, Hitte C, Galibert F, Kirkness E, Benson DW | title = Radiation hybrid mapping and genomic organization of canine TBX2 and TBX4 | journal = Animal Genetics | volume = 34 | issue = 4 | pages = 307–309 | date = August 2003 | pmid = 12873224 | doi = 10.1046/j.1365-2052.2003.01018.x }}
* {{cite journal | vauthors = Borke JL, Yu JC, Isales CM, Wagle N, Do NN, Chen JR, Bollag RJ | title = Tension-induced reduction in connexin 43 expression in cranial sutures is linked to transcriptional regulation by TBX2 | journal = Annals of Plastic Surgery | volume = 51 | issue = 5 | pages = 499–504 | date = November 2003 | pmid = 14595187 | doi = 10.1097/01.SAP.0000067964.14122.3E | s2cid = 21955969 }}
* {{cite journal | vauthors = Vance KW, Carreira S, Brosch G, Goding CR | title = Tbx2 is overexpressed and plays an important role in maintaining proliferation and suppression of senescence in melanomas | journal = Cancer Research | volume = 65 | issue = 6 | pages = 2260–2268 | date = March 2005 | pmid = 15781639 | doi = 10.1158/0008-5472.CAN-04-3045 | doi-access = free }}
* {{cite journal | vauthors = Bilican B, Goding CR | title = Cell cycle regulation of the T-box transcription factor tbx2 | journal = Experimental Cell Research | volume = 312 | issue = 12 | pages = 2358–2366 | date = July 2006 | pmid = 16730707 | doi = 10.1016/j.yexcr.2006.03.033 }}
{{refend}}

== External links ==
* {{MeshName|TBX2+protein,+human}}

{{PDB Gallery|geneid=6909}}
{{Transcription factors|g4}}

[[Category:Transcription factors]]

{{Sourceattribution|TBX2}}