View source for Medicine:Isolated organ perfusion technique

{{short description|Medical procedure}}
'''Isolated organ perfusion technique''' is employed to precipitate an organ's perfusion and circulation that are independent/isolated from the body's systemic circulation for various purposes such as organ-localized [[Medicine:Chemotherapy|chemotherapy]], organ-targeted delivery of drug, gene or anything else, [[Medicine:Organ transplantation|organ transplantation]], and organ injury recovery. The technique has been widely studied in animal and human for decades.<ref name="Cypel Keshavjee 2016 pp. 139–145">{{cite journal | last1=Cypel | first1=Marcelo | last2=Keshavjee | first2=Shaf | title=Novel Technologies for Isolated Lung Perfusion | journal=Thoracic Surgery Clinics | publisher=Elsevier BV | volume=26 | issue=2 | year=2016 | issn=1547-4127 | doi=10.1016/j.thorsurg.2015.12.002 | pages=139–145| pmid=27112253 }}</ref><ref name="Eiseman Knipe McCOLL Orloff 1961 pp. 356–363">{{cite journal | last1=Eiseman | first1=B. | last2=Knipe | first2=Peter | last3=McCOLL | first3=H. A. | last4=Orloff | first4=M. J. | title=Isolated Liver Perfusion for Reducing Blood Ammonia | journal=Archives of Surgery | volume=83 | issue=3 | date=1961-09-01 | issn=0004-0010 | doi=10.1001/archsurg.1961.01300150030004 | pages=356–363 | pmid=13726167 | url=https://jamanetwork.com/journals/jamasurgery/fullarticle/559744 | access-date=2019-06-30}}</ref><ref name="Harvard Apparatus">{{cite web | title=Isolated Organ Perfusion Studies - Physiology | website=Harvard Apparatus | url=https://www.harvardapparatus.com/physiology/isolated-organ-perfusion-studies.html | access-date=2019-06-30 | archive-url=https://web.archive.org/web/20190513041238/https://www.harvardapparatus.com/physiology/isolated-organ-perfusion-studies.html | archive-date=2019-05-13 | url-status=live }}</ref><ref name="Abbott Weinerth 1971 pp. 113–133">{{cite journal | last1=Abbott | first1=William M. | last2=Weinerth | first2=John L. | title=The perfusion of isolated whole organs | journal=Cryobiology | publisher=Elsevier BV | volume=8 | issue=2 | year=1971 | issn=0011-2240 | doi=10.1016/0011-2240(71)90019-8 | pages=113–133| pmid=5578880 }}</ref><ref name="Guyette Gilpin Charest Tapias pp. 1451–1468">{{cite journal | last1=Guyette | first1=Jacques P | last2=Gilpin | first2=Sarah E | last3=Charest | first3=Jonathan M | last4=Tapias | first4=Luis F | last5=Ren | first5=Xi | last6=Ott | first6=Harald C | title=Perfusion decellularization of whole organs | journal=Nature Protocols | publisher=Springer Science and Business Media LLC | volume=9 | issue=6 | date=2014-05-29 | issn=1754-2189 | doi=10.1038/nprot.2014.097 | pmid=24874812 | pages=1451–1468| s2cid=7397409 }}</ref><ref name="Schjørring Carlsson Simonsen 2015 pp. 277–295">{{cite book | last1=Schjørring | first1=Olav L. | last2=Carlsson | first2=Rune | last3=Simonsen | first3=Ulf | title=Methods in Mouse Atherosclerosis | volume=1339 | chapter=Pressure Myography to Study the Function and Structure of Isolated Small Arteries | series=Methods in Molecular Biology | publisher=Springer New York | location=New York, NY | year=2015 | isbn=978-1-4939-2928-3 | issn=1064-3745 | doi=10.1007/978-1-4939-2929-0_19 | pmid=26445796 | pages=277–295}}</ref><ref name="Serrat pp. 1749–1758">{{cite journal | last=Serrat | first=Maria A | title=Measuring bone blood supply in mice using fluorescent microspheres | journal=Nature Protocols | publisher=Springer Science and Business Media LLC | volume=4 | issue=12 | date=2009-11-05 | issn=1754-2189 | doi=10.1038/nprot.2009.190 | pmid=19893510 | pages=1749–1758| s2cid=29637802 }}</ref> Before the implementation, the perfusion system will be selected and the process can be similar to [[Chemistry:Organ bath|organ bath]].<ref>{{Cite web |url=https://www.harvardapparatus.com/media/harvard/pdf/Perfusion+Systems+Selection+Guide.pdf |title=Tissue bath |access-date=2019-06-30 |archive-url=https://web.archive.org/web/20170618163523/http://www.harvardapparatus.com/media/harvard/pdf/Perfusion+Systems+Selection+Guide.pdf |archive-date=2017-06-18 |url-status=live }}</ref> Isolated organ perfusion technique, nevertheless, is averagely conducted [[Biology:In vivo|in vivo]] without leaving the organ alone as a whole out of the body.<ref name="Yeh Wechsler 1998 pp. 435–452">{{cite book | last1=Yeh | first1=T. | last2=Wechsler | first2=A. S. | title=Surgical Research | chapter=The Isolated Organ in Research | publisher=Springer New York | location=New York, NY | year=1998 | isbn=978-1-4612-7325-7 | doi=10.1007/978-1-4612-1888-3_49 | pages=435–452|quote=Technological advances in artificial perfusion allow effective isolated perfusion of a wide variety of organs and tissues, including, but not limited to, brain, heart, lung, heart-lung, liver, kidney, spleen, pancreas, thymus, gastrointestinal tract, urinary tract, reproductive tract, skeletal muscle, nerves, and blood vessels. }}</ref>

==See also==
* ECMO

==References==
{{reflist}}

{{Vascular surgery procedures}}

[[Category:Oncology]]
[[Category:Organ donation]]
[[Category:Organ transplantation]]

{{Sourceattribution|Isolated organ perfusion technique}}