View source for Scanning electron cryomicroscopy

'''Scanning electron cryomicroscopy''' ('''CryoSEM''') is a  form of [[Physics:Electron microscopy|electron microscopy]] where a hydrated but cryogenically fixed sample is imaged on a [[Physics:Scanning electron microscope|scanning electron microscope]]'s cold stage in a cryogenic chamber. The cooling is usually achieved with [[Chemistry:Liquid nitrogen|liquid nitrogen]].<ref name="Dokland Terje">{{cite book | title=Techniques in Microscopy for Biomedical Applications | publisher=World Scientific Publishing Co Pte Ltd | author=Dokland Terje | year=2006 | pages=115 | isbn=978-9812564344}}</ref> CryoSEM of biological samples with a high moisture content can be done faster with fewer sample preparation steps than conventional SEM. In addition, the dehydration processes needed to prepare a biological sample for a conventional SEM chamber create numerous distortions in the tissue leading to structural artifacts during imaging.<ref name="Hickey2015">{{cite journal|last1=Hickey|first1=Cian D.|last2=Sheehan|first2=Jeremiah J.|last3=Wilkinson|first3=Martin G.|last4=Auty|first4=Mark A. E.|title=Growth and location of bacterial colonies within dairy foods using microscopy techniques: a review|journal=Frontiers in Microbiology|date=18 February 2015|volume=6|page=99 |doi=10.3389/fmicb.2015.00099|pmid=25741328 |pmc=4332360 |url=https://ulir.ul.ie/bitstream/10344/4511/2/Wilkinson_2015_growth.pdf|doi-access=free}}<!--|accessdate=12 March 2016--></ref><ref name="Lesemann">{{cite book|last1=Lesemann|first1=edited by Kurt Mendgen, Dietrich-Eckhardt|title=Electron Microscopy of Plant Pathogens|date=1991|publisher=Springer Berlin Heidelberg|location=Berlin, Heidelberg|isbn=978-3-642-75818-8}}</ref><ref name="Schatten2013">{{cite book|last1=Schatten|first1=Heide|title=Scanning electron microscopy for the life sciences|date=2013|publisher=Cambridge University Press|location=Cambridge|isbn=9780521195997|edition=Online-Ausg.}}</ref><ref name="Schatten">{{cite book|last1=editors|first1=Heide Schatten, James Pawley|title=Biological low voltage field emission scanning electron microscopy|date=2007|publisher=Springer|location=New York|isbn=9780387729725}}</ref>
==See also==
* [[Physics:Electron microscopy|Electron microscopy]]
* [[Engineering:Cryogenic electron microscopy|Electron cryomicroscopy]]
* [[Biology:Transmission electron cryomicroscopy|Transmission electron cryomicroscopy]]

==References==
{{reflist}}


[[Category:Electron microscopy]]
[[Category:Scientific techniques]]



{{Sourceattribution|Scanning electron cryomicroscopy}}