Biology:RDBP

Short description: Protein

Generic protein structure example

Negative elongation factor E is a protein that in humans is encoded by the RDBP gene.

Function

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC class III) region on chromosome 6.

Interactions

RDBP has been shown to interact with:

• Cofactor of BRCA1,
• TH1L, and
• WHSC2.

Further reading

• "Molecular genetics of the human MHC complement gene cluster". Experimental and Clinical Immunogenetics 15 (4): 213–30. 1999. doi:10.1159/000019075. PMID 10072631. 
• "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention". Medicinal Research Reviews 26 (5): 595–625. Sep 2006. doi:10.1002/med.20081. PMID 16838299. 
• "The human RD protein is closely related to nuclear RNA-binding proteins and has been highly conserved". Gene 90 (2): 299–302. Jun 1990. doi:10.1016/0378-1119(90)90194-V. PMID 2119325. 
• "Structure of the human RD gene: a highly conserved gene in the class III region of the major histocompatibility complex". DNA 8 (10): 745–51. Dec 1989. doi:10.1089/dna.1989.8.745. PMID 2612324. 
• "A previously undetected MHC gene with an unusual periodic structure". Science 240 (4849): 201–4. Apr 1988. doi:10.1126/science.3353717. PMID 3353717. Bibcode: 1988Sci...240..201L. 
• "cDNA cloning and characterization of the protein encoded by RD, a gene located in the class III region of the human major histocompatibility complex". The Biochemical Journal 294 (2): 589–93. Sep 1993. doi:10.1042/bj2940589. PMID 8373374. 
• "Genomic organization of the human DDX13 gene located between RD and RP1 in the class III MHC complex". Molecules and Cells 7 (3): 414–8. Jun 1997. PMID 9264031. 
• "A method for global protein expression and antibody screening on high-density filters of an arrayed cDNA library". Nucleic Acids Research 26 (21): 5007–8. Nov 1998. doi:10.1093/nar/26.21.5007. PMID 9776767. 
• "Four ubiquitously expressed genes, RD (D6S45)-SKI2W (SKIV2L)-DOM3Z-RP1 (D6S60E), are present between complement component genes factor B and C4 in the class III region of the HLA". Genomics 53 (3): 338–47. Nov 1998. doi:10.1006/geno.1998.5499. PMID 9799600. 
• "NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation". Cell 97 (1): 41–51. Apr 1999. doi:10.1016/S0092-8674(00)80713-8. PMID 10199401. 
• "DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation". The Journal of Biological Chemistry 276 (16): 12951–8. Apr 2001. doi:10.1074/jbc.M006130200. PMID 11112772. 
• "Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA". Molecular and Cellular Biology 22 (9): 2918–27. May 2002. doi:10.1128/MCB.22.9.2918-2927.2002. PMID 11940650. 
• "Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex". Molecular and Cellular Biology 23 (6): 1863–73. Mar 2003. doi:10.1128/MCB.23.6.1863-1873.2003. PMID 12612062. 
• "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse". Genome Research 13 (12): 2621–36. Dec 2003. doi:10.1101/gr.1736803. PMID 14656967. 
• "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics 83 (1): 153–67. Jan 2004. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819. 
• "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America 101 (33): 12130–5. Aug 2004. doi:10.1073/pnas.0404720101. PMID 15302935. Bibcode: 2004PNAS..10112130B. 
• "Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor". Genes & Development 18 (17): 2134–46. Sep 2004. doi:10.1101/gad.1214104. PMID 15342491. 

External links

• FactorBook NELFe