Biology:Bacteriophage pRNA
| Bacteriophage pRNA | |
|---|---|
 Predicted secondary structure and sequence conservation of Phage_pRNA | |
| Identifiers | |
| Symbol | Phage_pRNA |
| Rfam | RF00044 |
| Other data | |
| RNA type | Gene |
| Domain(s) | Viruses |
| SO | 0000655 |
| PDB structures | PDBe |
Bacteriophage pRNA is a ncRNA element. During replication of linear dsDNA viruses, the viral genome is packaged into the pre-formed viral procapsid. The packaging of DNA into the procapsid requires a molecular motor, which uses ATP as energy to accomplish the energetically unfavorable motion. In some bacteriophage, an RNA (pRNA) molecule is a vital component of this motor.[1] Structural analyses of the packaging motor have demonstrated that the pRNA molecule has fivefold symmetry when attached to the prohead.[2][3][4] The pRNA is thought to be bound by the capsid connector protein.[4] Only the first 120 bases of the pRNA are essential for packing the viral DNA.[5][6] The pRNA is proposed to be composed of two domains, one corresponding to the first 120 bases and the second to the remaining 50 bases.[6] Nuclear cleavage occurs in the single strand region linking these two domains.[6]
References
- ↑ Guo P (2002). Structure and function of phi29 hexameric RNA that drives the viral DNA packaging motor: review. Progress in Nucleic Acid Research and Molecular Biology. 72. 415–472. doi:10.1016/S0079-6603(02)72076-X. ISBN 978-0-12-540072-5.
- ↑ "Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi29". J. Struct. Biol. 135 (1): 38–46. 2001. doi:10.1006/jsbi.2001.4379. PMID 11562164.
- ↑ "Structure of the bacteriophage phi29 DNA packaging motor". Nature 408 (6813): 745–750. 2000. doi:10.1038/35047129. PMID 11130079. Bibcode: 2000Natur.408..745S.
- ↑ 4.0 4.1 "Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage phi29". Acta Crystallogr. D 56 (Pt 9): 1187–1190. 2000. doi:10.1107/S0907444900009239. PMID 10957642.
- ↑ "A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA". Science 236 (4802): 690–694. 1987. doi:10.1126/science.3107124. PMID 3107124. Bibcode: 1987Sci...236..690G.
- ↑ 6.0 6.1 6.2 "Phylogenetic analysis and secondary structure of the Bacillus subtilis bacteriophage RNA required for DNA packaging". J. Biol. Chem. 265 (36): 22365–22370. 1990. doi:10.1016/S0021-9258(18)45714-6. PMID 2125049.
External links
 |  |
