Biography:Bridget Carragher

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Short description: American physicist
Bridget Carragher
Bridget Carragher.jpg
Born17 June 1957
CitizenshipUSA
Alma materUniversity of the Witwatersrand, Johannesburg, S. Africa (B.Sc. and M.Sc. in physics)

Northwestern University, Evanston, Illinois (M.Sc. in physics)

University of Chicago, Chicago, Illinois (Ph.D.)
Scientific career
FieldsElectron Microscopy, Structural Biology

Bridget Olivia Carragher (born 17 June 1957) is a South African physicist specialized in electron microscopy.

Carragher is an adjunct professor at the Columbia University (New York City, NY) and the founder and Chief Operations Officer of NanoImaging Services, Inc. She is also the director of the National Resources for Automated Molecular Microscopy (NRAMM), director of the Simons Electron Microscopy Center at New York Structural Biology Center (New York City, NY) and PI at the National Center for CryoEM Access and Training.

Personal life

Carragher was born in South Africa . She lived in Ghana during her childhood and for one year in England. After earning her master's degree, she moved to the United States and has lived there since where she is a US citizen.

Carragher is married and has two children.

Education

Carragher studied physics at the University of the Witwatersrand, South Africa, from 1975 to 1981. She spent a year at Northwestern University, Evanston, Illinois, and graduated with a MSc in physics. In 1987 she gained her PhD in physics from University of Chicago, Chicago, Illinois.

Career

Carragher has spent much of her career in developing streamlined and automated electron microscopy (EM) methods aimed at improving both the quality of EM data and the accessibility of these techniques to the broader biological community.

After her Ph.D., she held several posts in industry and academia until moving to the Scripps Research Institute in 2001. Since 2002, she has served with Clint Potter as the director of the National Resource for Automated Molecular Microscopy (NRAMM), an NIH-funded national biotechnology research resource. The NRAMM specializes in developing and applying automated technologies for EM and providing training at all levels.

In 2007 Carragher co-founded NanoImaging Services, Inc. The company works in the area of providing microscopy services to the biopharmaceutical and biotechnology industries.

In 2015 Carragher and Clint moved their academic lab from The Scripps Research Institute to the New York Structural Biology Center, where they serve as co-directors of the Simons Electron Microscopy Center. In May 2018, they were awarded a U24 grant to start the National Center for CryoEM Access and Training (NCCAT).

In 2022 she was named a Founding Technical Director of the Chan Zuckerberg Institute for Advanced Biological Imaging.[1][2]

Positions and Employment
Year Position
1987 - 1988 Research Associate, U. Chicago
1988 - 1990 Senior Scientist, CEMAX Incorporated, Santa Clara, California
1990 - 1991 Assistant Research Neuroscientist, Dept. of Neuroscience, UCSD
1992 - 1998 Director, Optical Visualization Facility, Beckman Institute, UIUC
1994 - 2001 Adjunct Assistant Professor, Dept. of Cell and Structural Biology, UIUC
1996 - 1999 Senior Research Scientist, National Center for Supercomputing Applications, UIUC
1998 - 2001 Director, Imaging Technology Group, Beckman Institute, UIUC
2001 - 2012 Associate Professor, Dept. of Cell Biology, The Scripps Research Institute. La Jolla, CA
2003–present Director, National Resource for Automated Molecular Microcopy (NRAMM)
2007–present Founder and Chief Science Officer, NanoImaging Services, Inc.
2012 - 2014 Professor, Dept. of Integrative Structural and Computational Biology, The Scripps Research Institute. La Jolla, CA
2015–present Director, Simons Electron Microscopy Center, New York Structural Biology Center, NY, NY
2015–present Adjunct Professor, Columbia University, New York, NY
2018–present Co-PI, National Center for CryoEM Access and Training
2022–present Founding Technical Director, Chan Zuckerberg Institute for Advanced Biological Imaging
Other Experience and Professional Memberships
Year Activity
1993–present Editorial Board, Journal of Structural Biology
1993–present Member of various NIH/NSF Study Sections
1993 - 2000 Scientific Advisory Committee, National Center for Macromolecular Imaging, Baylor College of Medicine
1993, 1995 Co-organizer, Workshop on Advanced Computing for Biological Imaging, UIUC/NCSA
1995 Co-organizer, A Hands-on Workshop on Practical Stereological Techniques, UIUC
1995–present Member of Advisory Committee, HVEM Resource, Boulder, Co.
1996 Co-editor, Journal of Structural Biology Special Issue on “Software Resources for Image Processing and Visualization in Structural Biology”
1998–present Member of Advisory Committee, BMIRR, Wadsworth Center, Albany, NY
1997, 1999 Vice-chair, chair, Gordon Conference on 3DEM of Macromolecules
2003 Co-organizer, Multidisciplinary Workshop on Automated Particle Selection
2003 - 2014 Co-organizer, Biennial Workshop In Molecular Microscopy
2003 Co-editor, Journal of Structural Biology, Special Issues on Automated Particle Selection and Analytical Methods
2003 - 2005 NIH BBCB Study Section
2005 - 2007 NIH MI Study Section
2005–present Associate editor, Journal of Structural Biology
2011 - 2015 Scientific Advisory Committee, Transcontinental EM Initiative for Membrane Protein Structure
2012 - 2014 Advisory Board, National Canadian CryoEM Facility
2013 - 2015 External Advisory Committee, Theoretical and Computational Biophysics Group
2014–present Chair, EMDB Committee for cryoEM map challenge
2015–present Member, wwPDB Integrative/Hybrid Method (IHM) Task Force: Federation Subgroup
2015–present Member, Diamond Scientific Advisory Committee
2015–present Member, RCSB Protein Data Bank Advisory Committee
2015–present Member, External Advisory Board, National Center for X-ray Tomography
2016–present Editorial Board, Cell Chemical Biology
2017–present Member, NCXT Advisory Committee
2017–present Member, International Scientific Advisory Board, Max Planck Institute, Frankfurt
2017–present External Advisory Board, UTSW multi-user cryoEM facility
2017 Honorary Fellow of the Royal Microscopy Society
2017–present Editorial Board, Biophysical Journal
2018–present External Advisory Board, Harvard multi-user cryoEM facility

Contribution to Science

Throughout her career, Carragher has published 165 papers,[3][4] received several research funding grants and owns five patents.

Patents

  • Smith PE, Callahan MP, Daniel I, Potter CS, Carragher B, Suloway CJ, inventors; Robotic system for sequencing multiple specimens between a holding tray and microscope. 2005, US 2005/0107917 A1.
  • Mulder A, Carragher B, Potter CS, inventors; Characterization of particulates using electron microscopy and image processing methods. 2011, Provisional. Filed 8/17/2011.
  • Carragher B, Potter CS, Jain T, inventors; Preparation of specimen arrays on an EM grid. 2012, Provisional. Filed 1/17/2012.
  • Carragher B, Potter CS, Jain T, inventors; Superhydrophilic specimen grids for electron microscopy. 2012, Provisional. Filed 1/17/2012.
  • Carragher B, Potter CS, Jain T, Kahn P, Wiktor P, inventors; Apparatus and method for producing specimens for electron microscopy. 2012, Provisional. Filed 1/17/2012.

Contributions to the EM field

  1. Suloway, C; Pulokas, J; Fellmann, D; Cheng, A; Guerra, F; Quispe, J; Stagg, S; Potter, CS et al. (2005). "Automated molecular microscopy: the new Leginon system". J Struct Biol 151 (1): 41–60. doi:10.1016/j.jsb.2005.03.010. PMID 15890530. 
  2. Lander, GC; Stagg, SM; Voss, NR; Cheng, A; Fellmann, D; Pulokas, J; Yoshioka, C; Irving, C et al. (2009). "Appion: an integrated, database-driven pipeline to facilitate EM image processing". J Struct Biol 166 (1): 95–102. doi:10.1016/j.jsb.2009.01.002. PMID 19263523. 
  3. Lyumkis, D; Julien, JP; de Val, N; Cupo, A; Potter, CS; Klasse, PJ; Burton, DR; Sanders, RW et al. (2013). "Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer". Science 342 (6165): 1484–90. doi:10.1126/science.1245627. PMID 24179160. Bibcode: 2013Sci...342.1484L. 
  4. Noble, AJ; Wei, H; Dandey, VP; Zhang, Z; Tan, YZ; Potter, CS; Carragher, B (2018). "Reducing effects of particle adsorption to the air-water interface in cryo-EM.". Nat Methods 15 (10): 793–795. doi:10.1038/s41592-018-0139-3. PMID 30250056. 
  5. Scapin, G; Dandey, VP; Zhang, Z; Prosise, W; Hruza, A; Kelly, T; Mayhood, T; Strickland, C et al. (2018). "Structure of the insulin receptor-insulin complex by single-particle cryo-EM analysis". Nature 556 (7699): 122–5. doi:10.1038/nature26153. PMID 29512653. Bibcode: 2018Natur.556..122S. 

Additional selected contributions to the EM field

CryoEM automation

Carragher was an early advocate for automated methods for electron microscopy. She developed software to analyze poorly ordered sickle cell hemoglobin fibers[5] and went on to collaborate with Ron Milligan's group to develop a pipeline for helical processing (Phoelix).[6] Carragher and Potter led the development of Leginon,[7] a system for automated control and data acquisition from an electron microscope, and Appion,[8] a pipeline for single particle data processing.

New technologies for TEM

Carragher and her team have developed a number of technologies for transmission electron microscopy (TEM). These include Spotiton, an inkjet dispense and vitrification system for cryo-TEM,[9][10] robotic devices to load TEM specimens into the microscope,[11] liquid handling robots for controlling sample vitrification[12][13] and negative staining, and new substrates.[14]

Contributions to structural biology

Carragher has co-authored several papers in this area.[15]

Carragher's lab team includes a number of post-docs and graduate students . These researchers have contributed a number of papers to scientific journals.[16][17][18][19]

Contributions to Methodology:

Carragher has worked to validate and improve methods in the field. In particular, she has worked on a particle picking workshop,[20] the recent CTF challenge[21] and a number of workshops organized by the EMDB to discuss standards and validation.[22][23] She have also organized workshops, including the NRAMM biennial Advanced Methods Workshop.

Awards

In 2019, Carragher and Philip E Bateson were awarded the 2019 Microscopy Society of America Distinguished Scientist Award.[24]

In 2022, Carragher was awarded The Biophysical Society Innovation Award.[25]

References

  1. ↑ Chan Zuckerberg official website
  2. ↑ Nano Imaging Services website
  3. ↑ Neuro Tree website
  4. ↑ Science Direct Journal website, Paper of the Year awards 2004 and 2015
  5. ↑ Carragher, B.; Bluemke, D. A.; Gabriel, B.; Potel, M. J.; Josephs, R. (1988-01-20). "Structural analysis of polymers of sickle cell hemoglobin. I. Sickle hemoglobin fibers". Journal of Molecular Biology 199 (2): 315–331. doi:10.1016/0022-2836(88)90316-6. ISSN 0022-2836. PMID 3351926. 
  6. ↑ Carragher, B.; Whittaker, M.; Milligan, R. A. (January 1996). "Helical processing using PHOELIX". Journal of Structural Biology 116 (1): 107–112. doi:10.1006/jsbi.1996.0018. ISSN 1047-8477. PMID 8742731. 
  7. ↑ Suloway, Christian; Pulokas, James; Fellmann, Denis; Cheng, Anchi; Guerra, Francisco; Quispe, Joel; Stagg, Scott; Potter, Clinton S. et al. (July 2005). "Automated molecular microscopy: the new Leginon system". Journal of Structural Biology 151 (1): 41–60. doi:10.1016/j.jsb.2005.03.010. ISSN 1047-8477. PMID 15890530. 
  8. ↑ Lander, Gabriel C.; Stagg, Scott M.; Voss, Neil R.; Cheng, Anchi; Fellmann, Denis; Pulokas, James; Yoshioka, Craig; Irving, Christopher et al. (April 2009). "Appion: an integrated, database-driven pipeline to facilitate EM image processing". Journal of Structural Biology 166 (1): 95–102. doi:10.1016/j.jsb.2009.01.002. ISSN 1047-8477. PMID 19263523. 
  9. ↑ Jain, Tilak; Sheehan, Patrick; Crum, John; Carragher, Bridget; Potter, Clinton S. (July 2012). "Spotiton: A prototype for an integrated inkjet dispense and vitrification system for cryo-TEM". Journal of Structural Biology 179 (1): 68–75. doi:10.1016/j.jsb.2012.04.020. PMID 22569522. 
  10. ↑ Dandey, Venkata P.; Wei, Hui; Zhang, Zhening; Tan, Yong Zi; Acharya, Priyamvada; Eng, Edward T.; Rice, William J.; Kahn, Peter A. et al. (May 2018). "Spotiton: New features and applications". Journal of Structural Biology 202 (2): 161–169. doi:10.1016/j.jsb.2018.01.002. PMID 29366716. 
  11. ↑ Potter, Clinton S.; Pulokas, James; Smith, Paul; Suloway, Christian; Carragher, Bridget (June 2004). "Robotic grid loading system for a transmission electron microscope". Journal of Structural Biology 146 (3): 431–440. doi:10.1016/j.jsb.2004.02.002. ISSN 1047-8477. PMID 15099584. 
  12. ↑ Quispe, Joel; Damiano, John; Mick, Stephen E.; Nackashi, David P.; Fellmann, Denis; Ajero, Teddy G.; Carragher, Bridget; Potter, Clinton S. (October 2007). "An improved holey carbon film for cryo-electron microscopy". Microscopy and Microanalysis 13 (5): 365–371. doi:10.1017/S1431927607070791. ISSN 1431-9276. PMID 17900388. Bibcode: 2007MiMic..13..365Q. 
  13. ↑ Mulligan, Sean K.; Speir, Jeffrey A.; Razinkov, Ivan; Cheng, Anchi; Crum, John; Jain, Tilak; Duggan, Erika; Liu, Er et al. (August 2015). "Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles". Microscopy and Microanalysis 21 (4): 1017–1025. doi:10.1017/S1431927615014324. ISSN 1435-8115. PMID 26223550. Bibcode: 2015MiMic..21.1017M. 
  14. ↑ Razinkov, Ivan; Dandey, Venkat; Wei, Hui; Zhang, Zhening; Melnekoff, David; Rice, William J.; Wigge, Christoph; Potter, Clinton S. et al. (August 2016). "A new method for vitrifying samples for cryoEM". Journal of Structural Biology 195 (2): 190–198. doi:10.1016/j.jsb.2016.06.001. ISSN 1095-8657. PMID 27288865. 
  15. ↑ Science Direct Journal website, Paper of the Year awards 2004 and 2015
  16. ↑ Stagg, Scott M.; Gürkan, Cemal; Fowler, Douglas M.; LaPointe, Paul; Foss, Ted R.; Potter, Clinton S.; Carragher, Bridget; Balch, William E. (2006-01-12). "Structure of the Sec13/31 COPII coat cage". Nature 439 (7073): 234–238. doi:10.1038/nature04339. ISSN 1476-4687. PMID 16407955. Bibcode: 2006Natur.439..234S. 
  17. ↑ Mulder, Anke M.; Yoshioka, Craig; Beck, Andrea H.; Bunner, Anne E.; Milligan, Ronald A.; Potter, Clinton S.; Carragher, Bridget; Williamson, James R. (2010-10-29). "Visualizing Ribosome Biogenesis: Parallel Assembly Pathways for 30S Subunit". Science 330 (6004): 673–677. doi:10.1126/science.1193220. ISSN 0036-8075. PMID 21030658. Bibcode: 2010Sci...330..673M. 
  18. ↑ Moeller, Arne; Kirchdoerfer, Robert N.; Potter, Clinton S.; Carragher, Bridget; Wilson, Ian A. (2012-12-21). "Organization of the Influenza Virus Replication Machinery". Science 338 (6114): 1631–1634. doi:10.1126/science.1227270. ISSN 0036-8075. PMID 23180774. Bibcode: 2012Sci...338.1631M. 
  19. ↑ Lyumkis, Dmitry; Julien, Jean-Philippe; de Val, Natalia; Cupo, Albert; Potter, Clinton S.; Klasse, Per-Johan; Burton, Dennis R.; Sanders, Rogier W. et al. (2013-12-20). "Cryo-EM structure of a fully glycosylated soluble cleaved HIV-1 envelope trimer". Science 342 (6165): 1484–1490. doi:10.1126/science.1245627. ISSN 1095-9203. PMID 24179160. Bibcode: 2013Sci...342.1484L. 
  20. ↑ Zhu, Yuanxin; Carragher, Bridget; Glaeser, Robert M.; Fellmann, Denis; Bajaj, Chandrajit; Bern, Marshall; Mouche, Fabrice; de Haas, Felix et al. (January 2004). "Automatic particle selection: results of a comparative study". Journal of Structural Biology 145 (1–2): 3–14. doi:10.1016/j.jsb.2003.09.033. ISSN 1047-8477. PMID 15065668. 
  21. ↑ Marabini, Roberto; Carragher, Bridget; Chen, Shaoxia; Chen, James; Cheng, Anchi; Downing, Kenneth H.; Frank, Joachim; Grassucci, Robert A. et al. (June 2015). "CTF Challenge: Result Summary". Journal of Structural Biology 190 (3): 348–359. doi:10.1016/j.jsb.2015.04.003. ISSN 1047-8477. PMID 25913484. 
  22. ↑ Henderson, Richard; Sali, Andrej; Baker, Matthew L.; Carragher, Bridget; Devkota, Batsal; Downing, Kenneth H.; Egelman, Edward H.; Feng, Zukang et al. (2012-02-08). "Outcome of the First Electron Microscopy Validation Task Force Meeting". Structure 20-330 (2): 205–214. doi:10.1016/j.str.2011.12.014. ISSN 0969-2126. PMID 22325770. 
  23. ↑ Patwardhan, Ardan; Carazo, José-Maria; Carragher, Bridget; Henderson, Richard; Heymann, J Bernard; Hill, Emma; Jensen, Grant J; Lagerstedt, Ingvar et al. (December 2012). "Data management challenges in three-dimensional EM". Nature Structural & Molecular Biology 19 (12): 1203–1207. doi:10.1038/nsmb.2426. ISSN 1545-9993. PMID 23211764. 
  24. ↑ Microscopy Society Of America website
  25. ↑ BioPhysics.org website



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