Biology:Agouti coloration genetics

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Short description: Gene responsible for color variations in many species
A cat hair showing light and dark bands caused by alternating production of agouti-signaling protein and α-MSH.

The agouti gene, the Agouti-signaling protein (ASIP) is responsible for variations in color in many species. Agouti works with extension to regulate the color of melanin which is produced in hairs. The agouti protein causes red to yellow pheomelanin to be produced, while the competing molecule α-MSH signals production of brown to black eumelanin. In wildtype mice, alternating cycles of agouti and α-MSH production cause agouti coloration. Each hair has bands of yellow which grew during agouti production, and black which grew during α-MSH production. Wildtype mice also have light-colored bellies. The hairs there are a creamy color the whole length because the agouti protein was produced the whole time the hairs were growing.[1][2]

In mice and other species, loss of function mutations generally cause a darker color, while gain of function mutations cause a yellower coat.[3]

Mice

Both of these mice are viable yellow agouti avy; however, the mouse on the right does not express it due to epigenetic methylation.

As of 1979, there were 17 known alleles of agouti in mice.[4]

  • Lethal yellow Ay causes yellow coloration and obesity. It is dominant to all other alleles in the series. When homozygous, it is lethal early in development.[4]
  • Viable yellow Avy looks similar to lethal yellow and also causes obesity, but is not lethal when homozygous. Homozygous viable yellow mice can be variable in color from clear yellow through mottled black and yellow to a darker color similar to the agouti color.[4]
  • Intermediate yellow aiy causes a mottled yellow coloration, which like viable yellow can sometimes resemble agouti.[4]
  • Sienna yellow Asy heterozygotes are a dark yellow, while homozygotes are generally a clearer yellow.[4]
  • White-bellied agouti AW mice have agouti coloration, with hairs that are black at the tips, then yellow, then black again, and white to tan bellies.[4]
  • Agouti A looks like AW but the belly is dark like the back.[4]
  • Black and tan at causes a black back with a tan belly. A/at heterozygotes look like AW mice.[4]
  • Nonagouti a mice are almost completely black, with only a few yellow hairs around the ears and the genitals.[4]
  • Extreme nonagouti ae mice are fully black, and is recessive to all other alleles in the series.[4]

This is not a complete list of mouse agouti alleles.

The nonagouti allele a is unusually likely to revert to the black-and-tan allele at or to the white-bellied agouti allele AW.[5]

Agouti production is regulated by multiple different promoter regions, capable of promoting transcription just in the ventral (belly) area, as seen in white-bellied agouti and black-and-tan mice, or all across the body but just during a specific part of the hair growth cycle, as seen in agouti and white-bellied agouti.[6]

Lethal yellow and viable yellow cause obesity, features of type II diabetes, and a higher likelihood of tumors.[2] In normal mice Agouti is only expressed in the skin during hair growth, but these dominant yellow mutations cause it to be expressed in other tissues including liver, muscle, and fat.[7] The mahogany locus interacts with Agouti and a mutation there can override the pigmentation and body weight effects of lethal yellow.[8]

Viable yellow agouti mice can inherit epigenetic differences from their dam affecting how yellow or brown they become.[9]

The mouse agouti gene is found on chromosome 2.[2]

Dogs

An agouti dog, also called wolf sable

In dogs, the agouti gene is associated with various coat colors and patterns.[10]

The alleles at the A locus are related to the production of agouti-signaling protein (ASIP) and determine whether an animal expresses an agouti appearance and, by controlling the distribution of pigment in individual hairs, what type of agouti. There are four known alleles that occur at the A locus:

  • Ay = Fawn or sable (tan with black whiskers and varying amounts of black-tipped and/or all-black hairs dispersed throughout) - fawn typically referring to dogs with clearer tan and sable to those with more black shading
  • aw = Wild-type agouti (each hair with 3-6 bands alternating black and tan) - also called wolf sable
  • at = Tan point (black with tan patches on the face and underside) - including saddle tan (tan with a black saddle or blanket) [11][12]
  • a = Recessive black (black, inhibition of phaeomelanin)
  • ayt = Recombinant fawn (expresses a varied phenotype depending on the breed) has been identified in numerous Tibetan Spaniels and individuals in other breeds, including the Dingo. Its hierarchical position is not yet understood.[13][14][15]

Most texts suggest that the dominance hierarchy for the A locus alleles appears to be as follows: Ay > aw > at > a; however, research suggests the existence of pairwise dominance/recessiveness relationships in different families and not the existence of a single hierarchy in one family.[16]

  • Ay is incompletely dominant to at, so that heterozygous individuals have more black sabling, especially as puppies and Ayat can resemble the awaw phenotype. Other genes also affect how much black is in the coat.
  • aw is the only allele present in many Nordic spitzes, and is not present in most other breeds.
  • at includes tan point and saddle tan, both of which look tan point at birth. Modifier genes in saddle tan puppies cause a gradual reduction of the black area until the saddle tan pattern is achieved.
  • a is only present in a handful of breeds. Most black dogs are black due to a K locus allele.

A 2021 study found distinct genetic causes for fawn and sable, which it refers to as "dominant yellow" and "shaded yellow". Both have a more active hair cycle promoter than the wildtype agouti, but dominant yellow also has a more active ventral promoter. The hair cycle promoter involved in these colors is thought to have arisen about 2 million years ago in an extinct species of canid, which later hybridized with wolves.[17]

Cats

The dominant, wild-type A allows hairs to be banded with black and red (revealing the underlying tabby pattern), while the recessive non-agouti or "hypermelanistic" allele, a, causes black pigment production throughout the growth cycle of the hair.[18] Thus, the non-agouti genotype (aa) masks or hides the tabby pattern, although sometimes a suggestion of the underlying pattern can be seen (called "ghost striping"), especially in kittens. The sex-linked orange coloration is epistatic over agouti, and prevents the production of black pigment.

Agouti alleles in cats
Allele Symbol Image Description Mutation
Agouti A Arthur, the cat.jpg Tabby pattern thanks to a functional agouti gene. Wildtype
Nonagouti a Black cat 1 (1).jpg Black, which lacks a functional agouti gene and so cannot signal MC1R to produce red pigment. A 2 base pair frameshift deletion thought to cause a complete loss of function[19][20]

Horses

In normal horses, ASIP restricts the production of eumelanin to the "points": the legs, mane, tail, ear edges, etc.[21] In 2001, researchers discovered a recessive mutation on ASIP that, when homozygous, left the horse without any functional ASIP. As a result, horses capable of producing true black pigment had uniformly black coats.[22] The dominant, wildtype allele producing bay is symbolized as A, while the recessive allele producing black is symbolized as a. Extension is epistatic over agouti and will cause chestnut coloration regardless of what agouti alleles are present.

Agouti alleles in horses
Allele Symbol Image Description Mutation
Bay A BayMare.jpg Bay pattern due to a functional agouti gene. The body is red while the "points", the mane, tail, and lower legs, are black. Wildtype
Black a Champion Dales Pony.jpg Black horses produce black pigment in the entire coat because they lack a functional Agouti protein. An 11 base pair frameshift deletion in exon 2[22]

History

The cause behind the various shades of bay, particularly the genetic factors responsible for wild bay and seal brown, have been contested for over 50 years. In 1951, zoologist Miguel Odriozola published "A los colores del caballo" in which he suggested four possible alleles for the "A" gene, A+, A, At, and a, in order of most dominant to least.[23]

Additional hypothesized agouti alleles in horses
Name Symbol Image Description
Wild or Wildtype bay A+ Halterstandingshotarabianone.jpg Wildtype bay pattern, wherein the black points do not extend so far up the legs.
Seal brown At Kevin Tornado Zlosyn 2005.jpg Seal brown color, extremely dark brown coat color, distinguished from black by the presence of red on the flanks, muzzle, and around the eyes.
The pale areas on this bay horse are due to the pangaré trait

This was accepted until the 1990s, when a different hypothesis became popular.[24] It proposed that shades of bay were caused by many different genes, some which lightened the coat, some which darkened it. This theory also suggested that seal brown horses were black horses with a trait called pangare.[25] Pangaré is an ancestral trait also called "mealy", which outlines the soft or communicative parts of the horse in buff tan.

The combination of black and pangaré was dismissed as the cause of seal brown in 2001, when a French research team published Mutations in the agouti (ASIP), the extension (MC1R), and the brown (TYRP1) loci and their association to coat color phenotypes in horses (Equus caballus). This study used a DNA test to identify the recessive a allele on the Agouti locus, and found that none of the horses fitting the phenotype of seal brown were homozygous for the a allele.[22][26]

In 2007 one genetics lab began offering a test for what they believed was a marker for seal brown, and later for an agouti allele which they believed caused the brown color.[27][28] However, the underlying research was never published and the test was suspended by 2015 due to unreliable results.[29][30]

The genetic alleles that create seal brown and wildtype bay remain unknown. It is still hypothesized that to some extent, the darkening of coat color in some bays may be regulated by unrelated genes for traits like "sooty".[31]

Donkeys

Most donkeys have creamy to gray-white areas on the belly and around the muzzle and eyes, called light points or pangare. However, a recessive variant of agouti causes those areas to be the same color as the body in a pattern called no light points or NLP, which is similar to recessive black in other mammals. This allele can be found in Norman donkeys and American miniature donkeys.[32]

Agouti alleles in donkeys
Allele Symbol Image Description Mutation
Light points A Poland. Warsaw. Praga Północ 005.JPG A gray dun donkey with a white belly and white around the muzzle Wildtype
No light points anlp Donkey 002.jpg A gray dun donkey with no white areas A single nucleotide polymorphism pc.349 T > C[32]

Rabbits

In rabbits, the wildtype is agouti with a light belly, and a recessive non-agouti allele causes a black coat. A third allele, possibly a mutation to a regulator or promoter region, is thought to cause black and tan color. The nonagouti allele is estimated to have first appeared before 1700.[3]

Agouti is linked to the wideband gene, with about a 30% crossover rate.[33]

Like white bellied agouti mice, rabbits with wildtype agouti produce transcripts with different untranslated 5' ends that have different dorsal and ventral expression. The 1A exon is only expressed in the belly region and may be responsible for the lighter color there.[3]

Agouti alleles in rabbits
Allele Symbol Image Description Mutation
Agouti A Rabbit (agouti) 04.jpg Chestnut, sometimes called agouti. Hairs are banded black and yellow, and the belly is light. This resembles the mouse's light bellied agouti. Wildtype
Black and tan at LapinTitus.jpg Black otter, black with a white belly. Tan rabbits are all this color, but also have the wideband pattern and rufous color causing their bellies to be fiery orange. 11 kb deletion in hair-cycle-specific promoter region [34]
Nonagouti a Black rabbit.JPG Black self. All hairs are fully black. Single base pair frameshift insertion in exon 2 causing loss of function[3]

References

  1. ↑ "The interaction of agouti signal protein and melanocyte stimulating hormone to regulate melanin formation in mammals". Pigment Cell Research 9 (4): 191–203. August 1996. doi:10.1111/j.1600-0749.1996.tb00109.x. PMID 8948501. 
  2. ↑ 2.0 2.1 2.2 Online Mendelian Inheritance in Man (OMIM) 600201
  3. ↑ 3.0 3.1 3.2 3.3 "Characterization of the rabbit agouti signaling protein (ASIP) gene: transcripts and phylogenetic analyses and identification of the causative mutation of the nonagouti black coat colour". Genomics 95 (3): 166–75. March 2010. doi:10.1016/j.ygeno.2009.11.003. PMID 20004240. 
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Silvers, Willys K. (1979). "The Agouti and Extension series of Alleles, Umbrous and Sable". Springer Verlag. http://www.informatics.jax.org/wksilvers/frames/frame2-1.shtml. 
  5. ↑ Bultman, S. J.; Klebig, M. L.; Michaud, E. J.; Sweet, H. O.; Davisson, M. T.; Woychik, R. P. (1994). "Molecular analysis of reverse mutations from nonagouti (A) to black-and-tan (A(t)) and white-bellied agouti (Aw) reveals alternative forms of agouti transcripts". Genes & Development 8 (4): 481–490. doi:10.1101/gad.8.4.481. PMID 8125260. 
  6. ↑ Vrieling, H.; Duhl, D. M.; Millar, S. E.; Miller, K. A.; Barsh, G. S. (1994). "Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene". Proceedings of the National Academy of Sciences 91 (12): 5667–5671. doi:10.1073/pnas.91.12.5667. PMID 8202545. Bibcode: 1994PNAS...91.5667V. 
  7. ↑ "Ectopic expression of the agouti gene in transgenic mice causes obesity, features of type II diabetes, and yellow fur". Proceedings of the National Academy of Sciences of the United States of America 92 (11): 4728–32. May 1995. doi:10.1073/pnas.92.11.4728. PMID 7761391. Bibcode: 1995PNAS...92.4728K. 
  8. ↑ Gunn, Teresa M.; Miller, Kimberly A.; He, Lin; Hyman, Richard W.; Davis, Ronald W.; Azarani, Arezou; Schlossman, Stuart F.; Duke-Cohan, Jonathan S. et al. (1999). "The mouse mahogany locus encodes a transmembrane form of human attractin". Nature 398 (6723): 152–156. doi:10.1038/18217. PMID 10086356. Bibcode: 1999Natur.398..152G. 
  9. ↑ Morgan, Hugh D.; Sutherland, Heidi G.E.; Martin, David I.K.; Whitelaw, Emma (1999). "Epigenetic inheritance at the agouti locus in the mouse". Nature Genetics 23 (3): 314–318. doi:10.1038/15490. PMID 10545949. 
  10. ↑ "Dog Coat Colour Genetics". http://www.doggenetics.co.uk/tan.html. 
  11. ↑ "A SINE insertion causes the black-and-tan and saddle tan phenotypes in domestic dogs". The Journal of Heredity 102 (Suppl 1): S11-8. 2011. doi:10.1093/jhered/esr042. PMID 21846741. 
  12. ↑ "Identification of a mutation that is associated with the saddle tan and black-and-tan phenotypes in Basset Hounds and Pembroke Welsh Corgis". The Journal of Heredity 104 (3): 399–406. 2013. doi:10.1093/jhered/est012. PMID 23519866. 
  13. ↑ Dayna L Dreger (May 29, 2019). "True Colors: commercially-acquired morphological genotypes reveal hidden allele variation among dog breeds, informing both trait ancestry and breed potential". PLOS ONE 14 (10): e0223995. doi:10.1371/journal.pone.0223995. PMID 31658272. PMC 6816562. Bibcode: 2019PLoSO..1423995D. https://www.biorxiv.org/content/10.1101/654343v1.full#disqus_thread. Retrieved September 23, 2019. 
  14. ↑ Agouti Series
  15. ↑ Dayna L Dreger (Jul 3, 2020). "Atypical Genotypes for Canine Agouti Signaling Protein Suggest Novel Chromosomal Rearrangement". Genes 11 (7): 739. doi:10.3390/genes11070739. PMID 32635139. 
  16. ↑ "Characterization of the dog Agouti gene and a nonagoutimutation in German Shepherd Dogs". Mammalian Genome 15 (10): 798–808. October 2004. doi:10.1007/s00335-004-2377-1. PMID 15520882. 
  17. ↑ Bannasch, Danika L. et al. (2021). "Dog colour patterns explained by modular promoters of ancient canid origin". Nature Ecology & Evolution 5 (10): 1415–1423. doi:10.1038/s41559-021-01524-x. PMID 34385618. Bibcode: 2021NatEE...5.1415B. 
  18. ↑ "Molecular genetics and evolution of melanism in the cat family". Current Biology 13 (5): 448–53. March 2003. doi:10.1016/S0960-9822(03)00128-3. PMID 12620197. 
  19. ↑ "Molecular genetics and evolution of melanism in the cat family". Current Biology 13 (5): 448–53. 2003. doi:10.1016/S0960-9822(03)00128-3. PMID 12620197. 
  20. ↑ "Specifying and sustaining pigmentation patterns in domestic and wild cats". Science 337 (6101): 1536–41. September 2012. doi:10.1126/science.1220893. PMID 22997338. Bibcode: 2012Sci...337.1536K. 
  21. ↑ "Gene A: Distribution of Black Pigmented Hair". UC Davis Veterinary Genetics Laboratory. http://www.vgl.ucdavis.edu/services/coatcolor.php#genea. 
  22. ↑ 22.0 22.1 22.2 "Mutations in the agouti (ASIP), the extension (MC1R), and the brown (TYRP1) loci and their association to coat color phenotypes in horses (Equus caballus)". Mammalian Genome 12 (6): 450–5. June 2001. doi:10.1007/s003350020017. PMID 11353392. https://www.researchgate.net/publication/11985079. "The 11-bp deletion in ASIP exon 2 (ADEx2) alters the amino acid sequence and is believed to extend the regular termination signal by 210 bp to 612 bp. The frameshift initiated by the deletion results in a novel modified agouti-signaling-protein. ADEx2 was completely associated with horse recessive black coat color (Aa/Aa) in all horses typed so far". 
  23. ↑ "The palomino horse". Genetics 46 (9): 1143–50. September 1961. doi:10.1093/genetics/46.9.1143. PMID 13877241. PMC 1210264. http://www.genetics.org/cgi/reprint/46/9/1143.pdf. 
  24. ↑ "Starting Point". The Horse Colors Site. 2007. http://www.horsecolor.com/basics/starting_point.htm. 
  25. ↑ Sponenberg 2003, pg 123. Fig. 9.10. "The mealy effect generally is lighter and more yellow than residual nonblack areas (which tend to be redder) on very sooty horses."
  26. ↑ Nancy Castle (2008-03-01). "Brown/Bay Dun". Dun Central Station. http://www.duncentralstation.com/BrownBayDun.html. 
  27. ↑ "Equine Testing Services". Pet DNA Services of AZ. http://www.petdnaservicesaz.com/Equine.html. 
  28. ↑ "Believe in Brown". http://www.diomics.com/DIOMICS/PetDNA-Equine-Info_files/BrownPaintHorseJournal0410.pdf. 
  29. ↑ "Understanding Equine DNA and Agouti". http://www.petdnaservicesaz.com/equine-testing/understanding-equine-dna-and-agouti/. 
  30. ↑ "The Enigmatic Brown Horse | Color Genetics". http://colorgenetics.info/equine/enigmatic-brown-horse. 
  31. ↑ Nancy Castle (2008-03-01). "Brown/Bay Dun". Dun Central Station. http://www.duncentralstation.com/BrownBayDun.html. 
  32. ↑ 32.0 32.1 "A missense mutation in the agouti signaling protein gene (ASIP) is associated with the no light points coat phenotype in donkeys". Genetics, Selection, Evolution 47 (1): 28. April 2015. doi:10.1186/s12711-015-0112-x. PMID 25887951. 
  33. ↑ "Linkage of "WIDE-BAND" and "AGOUTI" genes: The Agouti Gene and a Modifier Shown to Be Situated on the Same Chromosome in the Domesticated Rabbit". Journal of Heredity 25 (12): 477–481. 1934. doi:10.1093/oxfordjournals.jhered.a103864. 
  34. ↑ Letko, A.; Ammann, B.; Jagannathan, V.; Henkel, J.; Leuthard, F.; Schelling, C.; Carneiro, M.; Drögemüller, C. et al. (2020). "A deletion spanning the promoter and first exon of the hair cycle-specific ASIP transcript isoform in black and tan rabbits". Animal Genetics 51 (1): 137–140. doi:10.1111/age.12881. PMID 31729778. https://onlinelibrary.wiley.com/doi/abs/10.1111/age.12881. 

Further reading

  • "Expression and transgenic studies of the mouse agouti gene provide insight into the mechanisms by which mammalian coat color patterns are generated". Development 121 (10): 3223–32. October 1995. doi:10.1242/dev.121.10.3223. PMID 7588057. 



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