Biology:Smash and Grab
From HandWiki
Smash and Grab is the name given to a technique developed by Charles S. Hoffman and Fred Winston used in molecular biology to rescue plasmids from yeast transformants into Escherichia coli, also known as E. coli, in order to amplify and purify them.[1] In addition, it can be used to prepare yeast genomic DNA (and DNA from tissue samples) for Southern blot analyses or polymerase chain reaction (PCR).[citation needed]
References
- ↑ C.S. Hoffman; F. Winston (1987). "A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.". Gene 57 (2–3): 267–72. doi:10.1016/0378-1119(87)90131-4. PMID 3319781.
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