Biology:LRRC8E
 Generic protein structure example |
Leucine-rich repeat-containing protein 8E is a protein that in humans is encoded by the LRRC8E gene.[1] Researchers have found out that this protein, along with the other LRRC8 proteins LRRC8A, LRRC8B, LRRC8C, and LRRC8D, is sometimes a subunit of the heteromer protein volume-regulated anion channel.[2] Volume-Regulated Anion Channels (VRACs) are crucial to the regulation of cell size by transporting chloride ions and various organic osmolytes, such as taurine or glutamate, across the plasma membrane,[3] and that is not the only function these channels have been linked to.
While LRRC8E is one of many proteins that can be part of VRAC, research has found that it is not as crucial to the activity of the channel in comparison to LRRC8A and LRRC8D.[4][5][6] However, while we know that LRRC8A and LRRC8D are necessary for VRAC function, other studies have found that they are not sufficient for the full range of usual VRAC activity.[7] This is where the other LRRC8 proteins come in, such as LRRC8E, as the different composition of these subunits affects the range of specificity for VRACs.[8][6]
In addition to its role in VRACs, the LRRC8 protein family is also associated with agammaglobulinemia-5.[9]
Specifically for LRRC8E, there has been a recent study that found that this gene was nominally associated with panic disorder.[10]
References
- ↑ "Entrez Gene: LRRC8A leucine rich repeat containing 8 family, member A". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=56262.
- ↑ "Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC". Science 344 (6184): 634–8. May 2014. doi:10.1126/science.1252826. PMID 24790029. Bibcode: 2014Sci...344..634V. http://edoc.mdc-berlin.de/14008/1/14008oa.pdf.
- ↑ "VRACs and other ion channels and transporters in the regulation of cell volume and beyond". Nature Reviews Molecular Cell Biology 17 (5): 293–307. May 2016. doi:10.1038/nrm.2016.29. PMID 27033257.
- ↑ "LRRC8A protein is indispensable for swelling-activated and ATP-induced release of excitatory amino acids in rat astrocytes". The Journal of Physiology 592 (22): 4855–62. November 2014. doi:10.1113/jphysiol.2014.278887. PMID 25172945.
- ↑ "Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish =". Physiological Reports 4 (19): 16–27. October 2016. doi:10.14814/phy2.12940. PMID 27688432.
- ↑ 6.0 6.1 "Subunit composition of VRAC channels determines substrate specificity and cellular resistance to Pt-based anti-cancer drugs". The EMBO Journal 34 (24): 2993–3008. December 2015. doi:10.15252/embj.201592409. PMID 26530471.
- ↑ "Specific and essential but not sufficient roles of LRRC8A in the activity of volume-sensitive outwardly rectifying anion channel (VSOR)". Channels 11 (2): 109–120. March 2017. doi:10.1080/19336950.2016.1247133. PMID 27764579.
- ↑ "Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels". Journal of Cell Science 130 (6): 1122–1133. March 2017. doi:10.1242/jcs.196253. PMID 28193731.
- ↑ "A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans". The Journal of Clinical Investigation 112 (11): 1707–13. December 2003. doi:10.1172/JCI18937. PMID 14660746.
- ↑ "Association between genes on chromosome 19p13.2 and panic disorder". Psychiatric Genetics 26 (6): 287–292. December 2016. doi:10.1097/YPG.0000000000000147. PMID 27610895.
Further reading
- "The LIFEdb database in 2006". Nucleic Acids Research 34 (Database issue): D415–8. January 2006. doi:10.1093/nar/gkj139. PMID 16381901.
- "From ORFeome to biology: a functional genomics pipeline". Genome Research 14 (10B): 2136–44. October 2004. doi:10.1101/gr.2576704. PMID 15489336.
- "DNA cloning using in vitro site-specific recombination". Genome Research 10 (11): 1788–95. November 2000. doi:10.1101/gr.143000. PMID 11076863.
- "Cellular function and control of volume-regulated anion channels". Cell Biochemistry and Biophysics 35 (3): 263–74. 2001. doi:10.1385/CBB:35:3:263. PMID 11894846.
- "Reciprocal translocation versus centric fusion between two No. 13 chromosomes. A case of 46,XX,-13,+t(13;13)(p12;q13) and a case of 46,XY,-13,+t(13;13)(p12;p12)". Cytogenetics and Cell Genetics 12 (4): 235–44. 2016. doi:10.1007/s00424-015-1765-6. PMID 4752865.
- "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Research 7 (1): 65–73. February 2000. doi:10.1093/dnares/7.1.65. PMID 10718198.
- "LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins". FEBS Letters 564 (1–2): 147–52. April 2004. doi:10.1016/S0014-5793(04)00332-1. PMID 15094057.
- "LRRC8 extracellular domain is composed of 17 leucine-rich repeats". Molecular Immunology 41 (5): 561–2. July 2004. doi:10.1016/j.molimm.2004.04.001. PMID 15183935.
- "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Research 12 (2): 117–26. 2007. doi:10.1093/dnares/12.2.117. PMID 16303743.
- "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. November 2006. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
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