Biology:BioPlex
BioPlex (biophysical interactions of ORFeome-based complexes) is an open access resource for studying protein-protein interactions.[1][2][3] It is the result of collaborations between Harvard Medical School and Biogen. BioPlex 1.0 reported 23,744 interactions among 7,668 proteins.[4] BioPlex 2.0 extended those observations to detect over 29,000 new interactions.[5]
The basic technology is to express a "bait" protein in human cells. Those bait proteins interact with other proteins, and then the complexes of the bait and "prey" proteins are isolated by affinity purification. The interacting prey proteins are identified using mass spectrometry.
Use in research
Determining the interaction partners of poorly characterized proteins can provide clues to the function of those proteins, and knowing which "protein community" a disease gene resides in can give better context for its action.[5]
See also
References
- ↑ anon. "BioPlex". harvard.edu. http://bioplex.hms.harvard.edu/index.php.
- ↑ anon (2015-07-18). "Facebook for the Proteome". technology.org. http://www.technology.org/2015/07/18/facebook-for-the-proteome/.
- ↑ anon. "New study maps protein interactions for a quarter of the human genome". phys.org. https://phys.org/news/2017-05-protein-interactions-quarter-human-genome.html.
- ↑ "The BioPlex Network: A Systematic Exploration of the Human Interactome". Cell 162 (2): 425–40. 2015. doi:10.1016/j.cell.2015.06.043. PMID 26186194.
- ↑ 5.0 5.1 "Architecture of the human interactome defines protein communities and disease networks". Nature 545 (7655): 505–509. 2017. doi:10.1038/nature22366. PMID 28514442. Bibcode: 2017Natur.545..505H.
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