Biology:Calf-intestinal alkaline phosphatase
| Calf-intestinal alkaline phosphatase | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | ALPI | ||||||
| UniProt | P19111 | ||||||
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| Calf-intestinal alkaline phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 3.1.3.1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands and phosphomonoesters from RNA.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate.[3] This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.
Applications
Calf-intestinal alkaline phosphatase can serve as an effective tool for removing uranium from groundwater and soil that can pose major health risks.[4] Furthermore, the toxicity of lipopolysaccharide (LPS) was mitigated by calf-intestinal alkaline phosphatase in mice and piglets, which indicates that it could be a promising new therapeutic agent for treating diseases associated with LPS.[5]
References
- ↑ Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory. 1989.
- ↑ "Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone". Nature 270 (5637): 486–494. December 1977. doi:10.1038/270486a0. PMID 339105. Bibcode: 1977Natur.270..486S.
- ↑ "Rat insulin genes: construction of plasmids containing the coding sequences". Science (New York, N.Y.) 196 (4296): 1313–1319. June 1977. doi:10.1126/science.325648. PMID 325648. Bibcode: 1977Sci...196.1313U.
- ↑ "A Novel Approach to Precipitation of Heavy Metals from Industrial Effluents and Single-Ion Solutions Using Bacterial Alkaline Phosphatase" (in en). Water, Air, & Soil Pollution 224 (7): 1625. July 2013. doi:10.1007/s11270-013-1625-y. ISSN 0049-6979. Bibcode: 2013WASP..224.1625C. http://link.springer.com/10.1007/s11270-013-1625-y.
- ↑ "Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets". The Journal of Pharmacology and Experimental Therapeutics 307 (2): 737–744. November 2003. doi:10.1124/jpet.103.056606. PMID 12970380.
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