Biology:Ham's tissue culture medium

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Short description: Growth medium for mammalian cells

Ham's tissue culture medium is a growth medium for mammalian cells.[1]

Contents

It contains in amounts dissolved in 1 liter of triple distilled water:

L-Arginine 211 mg
Biotin 0.024 mg
L-Histidine 21 mg
Calcium pantothenate 0.7 mg
L-Lysine 29.3 mg
Choline chloride 0.69 mg
L-Methionine 4.48 mg
i-inositol 0.54 mg
L-Phenylalanine 4.96 mg
Niacinamide 0.6 mg
L-Tryptophan 0.6 mg
Pyridoxine hydrochloride 0.2 mg
L-Tyrosine 1.81 mg
Riboflavin 0.37 mg
L-Alanine 8.91 mg
Thymidine 0.7 mg
Glycine 7.51 mg
Cyanocobalamin 1.3 mg
L-Serine 10.5 mg
Sodium pyruvate 110 mg
L-Threonine 3.57 mg
Lipoic acid 0.2 mg
L-Aspartic acid 13.3 mg
CaCl2 44 mg
L-Glutamic acid 14.7 mg
MgSO4·7H2O 153 mg
L-Asparagine 15 mg
Glucose 1.1 g
L-Glutamine 146.2 mg
NaCl 7.4 g
L-Isoleucine 2.6 mg
KCl 285 mg
L-Leucine 13.1 mg
Na2HPO4 290 mg
L-Proline 11.5 mg
KH2PO4 83 mg
L-Valine 3.5 mg
Phenol red 1.2 mg
L-Cysteine 31.5 mg
FeSO4 0.83 mg
Thiamine hydrochloride 1 mg
CuSO4·5H2O 0.0025 mg
Hypoxanthine 4 mg
ZnSO4·7H2O 0.028 mg
Folic acid 1.3 mg
NaHCO3 1.2 g

See also

  • Biotechnology
  • Brewing
  • Cell biology
  • DMEM Dulbecco's Modified Eagle's Medium
  • Eagle's minimal essential medium (a minimum ingredient cell culture medium)
  • Fermentation (food)
  • GMEM (Glasgow's Minimal Essential Medium)
  • Murashige and Skoog medium (Plant cell culture medium)
  • RPMI (Roswell Park Memorial Institute medium), for lymph cells

References

  1. Shenoy, Manjula (2007) (in en). Animal Biotechnology. Firewall Media. pp. 19–20. ISBN 978-81-318-0108-6. https://books.google.com/books?id=3d7UnzvaDREC. 

Further reading