Biology:IFT20
 Generic protein structure example |
Intraflagellar transport protein 20 homolog is a protein that in humans is encoded by the IFT20 gene.[1] The gene is composed of 6 exons and is located on human chromosome 17p11.1. This gene is expressed in human brain, lung, kidney and pancreas, and lower expression were also detected in human placenta, liver, thymus, prostate and testis.[2]
Intraflagellar transport (IFT), in which molecular motors and IFT particle proteins participate, is very important in assembling and maintaining many cilia/flagella, such as the motile cilia that drive the swimming of cells and embryos, the nodal cilia that generate left-right asymmetry in vertebrate embryos, and the sensory cilia that detect sensory stimuli in some animals.[2] IFT20 subunit of the particle is localized to the Golgi complex in addition to the basal body and cilia where all previous IFT particle proteins had been found. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules.[3] IFT20 has been shown to interact with SPEF2 in the testis, and plays a role in sperm motility.[4]
References
- ↑ "Entrez Gene: IFT20 intraflagellar transport 20 homolog (Chlamydomonas)". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=90410.
- ↑ 2.0 2.1 "Cloning and characterization of the human IFT20 gene". Mol. Biol. Rep. 30 (4): 255–60. December 2003. doi:10.1023/A:1026365124176. PMID 14672413.
- ↑ "The Intraflagellar Transport Protein IFT20 Is Associated with the Golgi Complex and Is Required for Cilia Assembly". Mol. Biol. Cell 17 (9): 3781–92. 2006. doi:10.1091/mbc.E06-02-0133. PMID 16775004.
- ↑ Sironen, Anu; Hanse, J.; Thomsen, B.; Andersson, M.; Vilkki, J.; Toppari, J.; Kotaja, N (2010). "Expression of SPEF2 During Mouse Spermatogenesis and Identification of IFT20 as an Interacting Protein". Biology of Reproduction 82 (3): 580–590. doi:10.1095/biolreprod.108.074971. PMID 19889948.
Further reading
- Andersson B; Wentland MA; Ricafrente JY et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
- Yu W; Andersson B; Worley KC et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMID 9110174.
- Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M.
- Baker SA; Freeman K; Luby-Phelps K et al. (2003). "IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia". J. Biol. Chem. 278 (36): 34211–8. doi:10.1074/jbc.M300156200. PMID 12821668.
- Jurczyk A; Gromley A; Redick S et al. (2004). "Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly". J. Cell Biol. 166 (5): 637–43. doi:10.1083/jcb.200405023. PMID 15337773.
- Gerhard DS; Wagner L; Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
- Rual JF; Venkatesan K; Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R.
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