Biology:MeRIPseq
From HandWiki
MeRIPseq[1] (or MeRIP-seq) stands for methylated RNA immunoprecipitation sequencing, which is a method for detection of post-transcriptional RNA modifications, developed by Kate Meyer et al. while working in the laboratory of Sammie Jaffrey at Cornell University Graduate School of Medical Sciences. It is also called m6A-seq.[2] A variation of the MerIP-seq method was coined by Benjamin Delatte and colleagues in 2016. This variant, called hMerIP-seq (hydroxymethylcytosine RNA immunoprecipitation), uses an antibody that specifically recognizes 5-hydroxymethylcytosine, a modified RNA base affecting in vitro translation and brain development in Drosophila.[3]
References
- ↑ Meyer, Kate D.; Saletore, Yogesh; Zumbo, Paul; Elemento, Olivier; Mason, Christopher E.; Jaffrey, Samie R. (31 May 2012). "Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons". Cell 149 (7): 1635–1646. doi:10.1016/j.cell.2012.05.003. PMID 22608085.
- ↑ Dominissini, Dan; Moshitch-Moshkovitz, Sharon; Schwartz, Schraga; Salmon-Divon, Mali; Ungar, Lior; Osenberg, Sivan; Cesarkas, Karen; Jacob-Hirsch, Jasmine et al. (28 April 2012). "Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq". Nature 485 (7397): 201–206. doi:10.1038/nature11112. PMID 22575960. Bibcode: 2012Natur.485..201D.
- ↑ Delatte, Benjamin (2016). "Transcriptome-wide distribution and function of RNA hydroxymethylcytosine". Science 351 (6270): 282–285. doi:10.1126/science.aac5253. PMID 26816380. Bibcode: 2016Sci...351..282D.
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