File:CBTE.jpg
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Summary
| Description |
English: DELs in cells: mRNA encoding the target fused to a small protein, the Prey is injected into Xenopus oocytes. After expression, library and Bait DNA is injected into the cell for binding. Then, the cell is lysed, the contents diluted, oil and surfactant added, and an emulsion formed. Bound library members and Bait will be trapped together in the droplets in the emulsion, whereas unbound library members will only be trapped together with Bait by chance. The binding event is then encoded by ligation of the Bait DNA to the library DNA, and subsequently, the ligated DNA is sequenced, allowing identification of library molecules binding the target. |
| Date | |
| Source | Own work |
| Author | Leif Kongskov Larsen |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 08:36, 23 May 2025 | | 4,091 × 1,939 (436 KB) | imagescommonswiki>Leif Kongskov Larsen | Uploaded while editing "DNA-encoded chemical library" on en.wikipedia.org |
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