Medicine:MiCK assay
Micro-Culture Kinetic (MiCK) assay, developed by DiaTech Oncology, is a clinical pathology test that measures apoptosis induced in specific patient's cancer cells via chemotherapy. The assay is performed by DiaTech Oncology in its CLAI-certified, CAP-accredited laboratory. The MiCK assay provides oncologists with a clinically relevant drug-sensitivity profile of tumor cells within an individual cancer patient.[citation needed]
For a proper MiCK assay to be performed, the oncologist will send a sample of the specimen (the type of the specimen depends on the type of the cancer) to DiaTech Oncology which will isolate and purify the tumor cells. It is a non-invasive test performed on a small portion of fresh live tumor from tissue, fluids, blood or a sample of bone marrow during the original surgery, indicating that no additional invasive procedure is required.
Once the sample specimen has been received, the tumor cells of an individual patient will be exposed, in vitro, to therapeutic doses of chemotherapy drugs. Once the MiCK assay has been performed, it suggests which chemotherapeutic agent(s) is required to kill the individual patient's specific cancer cells revealed by the developed profile. The MiCK assay results help oncologists choose a chemotherapeutic agent that is most efficient in killing the patient's specific cancer cells.
References
Further reading
- Kravtsov, Vladimir D.; Greer, John P.; Whitlock, James A.; Koury, Mark J. (1998). "Use of the Microculture Kinetic Assay of Apoptosis to Determine Chemosensitivities of Leukemias". Blood 92 (3): 968–80. doi:10.1182/blood.V92.3.968. PMID 9680366. http://bloodjournal.hematologylibrary.org/cgi/pmidlookup?view=long&pmid=9680366.
- Bosserman, Linda D.; Rajurkar, Swapnil P.; Rogers, Karl; Davidson, Dirk C.; Chernick, Michael; Hallquist, Allan; Malouf, Debbee; Presant, Cary A. (2012). "Correlation of drug-induced apoptosis assay results with oncologist treatment decisions and patient response and survival". Cancer 118 (19): 4877–83. doi:10.1002/cncr.27444. PMID 22354845.
- Bosserman, Linda; Prendergast, Franklyn; Herbst, Roy; Fleisher, Martin; Salom, Emery; Strickland, Steven; Raptis, Anastasios; Hallquist, Allan et al. (2012). "The Microculture-Kinetic (MiCK) Assay: The Role of a Drug-Induced Apoptosis Assay in Drug Development and Clinical Care". Cancer Research 72 (16): 3901–3905. doi:10.1158/0008-5472.CAN-12-0681. PMID 22865459.
- Strickland, Stephen A.; Raptis, Anastasios; Hallquist, Allan; Rutledge, James; Chernick, Michael; Perree, Mathieu; Talbott, Mahsa S.; Presant, Cary A. (2012). "Correlation of the microculture-kinetic drug-induced apoptosis assay with patient outcomes in initial treatment of adult acute myelocytic leukemia". Leukemia & Lymphoma 54 (3): 528–34. doi:10.3109/10428194.2012.722217. PMID 22924433.
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