View source for Biology:Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)

{{Infobox enzyme
| Name       = Pyridoxal 5′-phosphate synthase (glutamine hydrolyzing)
| EC_number  = 4.3.3.6
| CAS_number =
| GO_code    = 
| image      = 
| width      = 
| caption    =  
}}
'''Pyridoxal 5′-phosphate synthase (glutamine hydrolysing)''' (EC 4.3.3.6, ''PdxST'') is an [[Biology:Enzyme|enzyme]] with [[Biology:List of enzymes|systematic name]] '''<small>D</small>-ribose 5-phosphate,<small>D</small>-glyceraldehyde 3-phosphate pyridoxal 5′-phosphate-lyase'''.<ref>{{cite journal | vauthors = Burns KE, Xiang Y, Kinsland CL, McLafferty FW, Begley TP | title = Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway | journal = Journal of the American Chemical Society | volume = 127 | issue = 11 | pages = 3682–3 | date = March 2005 | pmid = 15771487 | doi = 10.1021/ja042792t }}</ref><ref>{{cite journal | vauthors = Raschle T, Amrhein N, Fitzpatrick TB | title = On the two components of pyridoxal 5'-phosphate synthase from ''Bacillus subtilis'' | journal = The Journal of Biological Chemistry | volume = 280 | issue = 37 | pages = 32291–300 | date = September 2005 | pmid = 16030023 | doi = 10.1074/jbc.m501356200 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Strohmeier M, Raschle T, Mazurkiewicz J, Rippe K, Sinning I, Fitzpatrick TB, Tews I | title = Structure of a bacterial pyridoxal 5′-phosphate synthase complex | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 103 | issue = 51 | pages = 19284–9 | date = December 2006 | pmid = 17159152 | pmc = 1748218 | doi = 10.1073/pnas.0604950103 | doi-access = free }}</ref><ref>{{cite journal | vauthors = Raschle T, Arigoni D, Brunisholz R, Rechsteiner H, Amrhein N, Fitzpatrick TB | title = Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate | journal = The Journal of Biological Chemistry | volume = 282 | issue = 9 | pages = 6098–105 | date = March 2007 | pmid = 17189272 | doi = 10.1074/jbc.m610614200 | url = http://www.zora.uzh.ch/24624/2/Raschle2007V.pdf | doi-access = free }}</ref><ref>{{cite journal | vauthors = Hanes JW, Keresztes I, Begley TP | title = Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate | journal = Angewandte Chemie | volume = 47 | issue = 11 | pages = 2102–5 | year = 2008 | pmid = 18260082 | doi = 10.1002/anie.200704390 }}</ref><ref>{{cite journal | vauthors = Hanes JW, Burns KE, Hilmey DG, Chatterjee A, Dorrestein PC, Begley TP | title = Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate | journal = Journal of the American Chemical Society | volume = 130 | issue = 10 | pages = 3043–52 | date = March 2008 | pmid = 18271580 | doi = 10.1021/ja076604l }}</ref><ref>{{cite journal | vauthors = Hanes JW, Keresztes I, Begley TP | title = 13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase | journal = Nature Chemical Biology | volume = 4 | issue = 7 | pages = 425–30 | date = July 2008 | pmid = 18516049 | doi = 10.1038/nchembio.93 }}</ref><ref>{{cite journal | vauthors = Wallner S, Neuwirth M, Flicker K, Tews I, Macheroux P | title = Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from ''Bacillus subtilis'' | journal = Biochemistry | volume = 48 | issue = 9 | pages = 1928–35 | date = March 2009 | pmid = 19152323 | doi = 10.1021/bi801887r }}</ref> This enzyme [[Chemistry:Catalysis|catalyses]] the following [[Chemistry:Chemical reaction|chemical reaction]]

: <small>D</small>-ribose 5-phosphate + <small>D</small>-glyceraldehyde 3-phosphate + <small>L</small>-glutamine <math>\rightleftharpoons</math> pyridoxal 5′-phosphate + <small>L</small>-glutamate + 3 H<sub>2</sub>O + phosphate (overall reaction)
::(1a) <small>L</small>-glutamine + H<sub>2</sub>O <math>\rightleftharpoons</math> <small>L</small>-glutamate + NH<sub>3</sub>
::(1b) <small>D</small>-ribose 5-phosphate + <small>D</small>-glyceraldehyde 3-phosphate + NH<sub>3</sub> <math>\rightleftharpoons</math> pyridoxal 5′-phosphate + 4 H<sub>2</sub>O + [[Chemistry:Phosphate|phosphate]]

The enzyme can also use [[Chemistry:Ribulose 5-phosphate|ribulose 5-phosphate]] and [[Chemistry:Dihydroxyacetone phosphate|dihydroxyacetone phosphate]].

== References ==
{{reflist}}

== External links ==
* {{MeshName|Pyridoxal+5'-phosphate+synthase+(glutamine+hydrolyzing)}}

{{Carbon-nitrogen lyases}}
{{Enzymes}}

[[Category:EC 4.3.3]]

{{Sourceattribution|Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)}}