Biology:Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)
From HandWiki
| Pyridoxal 5′-phosphate synthase (glutamine hydrolyzing) | |||||||||
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| Identifiers | |||||||||
| EC number | 4.3.3.6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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Pyridoxal 5′-phosphate synthase (glutamine hydrolysing) (EC 4.3.3.6, PdxST) is an enzyme with systematic name D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5′-phosphate-lyase.[1][2][3][4][5][6][7][8] This enzyme catalyses the following chemical reaction
- D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine [math]\displaystyle{ \rightleftharpoons }[/math] pyridoxal 5′-phosphate + L-glutamate + 3 H2O + phosphate (overall reaction)
- (1a) L-glutamine + H2O [math]\displaystyle{ \rightleftharpoons }[/math] L-glutamate + NH3
- (1b) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 [math]\displaystyle{ \rightleftharpoons }[/math] pyridoxal 5′-phosphate + 4 H2O + phosphate
The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate.
References
- ↑ "Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway". Journal of the American Chemical Society 127 (11): 3682–3. March 2005. doi:10.1021/ja042792t. PMID 15771487.
- ↑ "On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis". The Journal of Biological Chemistry 280 (37): 32291–300. September 2005. doi:10.1074/jbc.m501356200. PMID 16030023.
- ↑ "Structure of a bacterial pyridoxal 5′-phosphate synthase complex". Proceedings of the National Academy of Sciences of the United States of America 103 (51): 19284–9. December 2006. doi:10.1073/pnas.0604950103. PMID 17159152.
- ↑ "Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate". The Journal of Biological Chemistry 282 (9): 6098–105. March 2007. doi:10.1074/jbc.m610614200. PMID 17189272. http://www.zora.uzh.ch/24624/2/Raschle2007V.pdf.
- ↑ "Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate". Angewandte Chemie 47 (11): 2102–5. 2008. doi:10.1002/anie.200704390. PMID 18260082.
- ↑ "Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate". Journal of the American Chemical Society 130 (10): 3043–52. March 2008. doi:10.1021/ja076604l. PMID 18271580.
- ↑ "13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase". Nature Chemical Biology 4 (7): 425–30. July 2008. doi:10.1038/nchembio.93. PMID 18516049.
- ↑ "Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis". Biochemistry 48 (9): 1928–35. March 2009. doi:10.1021/bi801887r. PMID 19152323.
External links
- Pyridoxal+5'-phosphate+synthase+(glutamine+hydrolyzing) at the US National Library of Medicine Medical Subject Headings (MeSH)
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