Software:MasterPlex ReaderFit

MasterPlex ReaderFit

Developer(s)	MiraiBio Group of Hitachi Solutions America, Ltd.
Stable release	2.0.0.66 / May 27, 2011
Operating system	Windows
Type	Scientific graphing, statistics, and nonlinear regression (curve fitting) for elisa assay analysis
License	Proprietary
Website	http://www.MiraiBio.com/Elisa-Curve-Fit

MasterPlex ReaderFit is a commercial curve-fitting software designed specifically for elisa assay analysis published by the MiraiBio Group of Hitachi Solutions America, Ltd.

Features

• Full support for four parameter logistic (4 PL) and five parameter logistic (5 PL) nonlinear regression models with many options (remove outliers, compare curves, interpolate standard curves, extrapolate standard curves, enter dilution factors, etc.)
• Automatic EC50 and IC50 calculations for dose response curves
• Weighting algorithms are provided to optimize the curve fit and account for heteroscedasticity
• Quality control feature to automatically flag outliers based on lower limit of detection (llod), %CV, extrapolated values, etc.
• 'Best Fit' automatically selects the optimal model equation and weighting algorithm with different parameters to optimize for: Root mean square error (RMSE), R squared, and Standard Deviation of % Recovery
• Templates allow for users to save plate layout and standard/sample information saving time for subsequent analyses
• Spreadsheet style pivot tables allow for easy viewing and manipulation of results
• Merge multiple plate files to allow for cross-plate analyses (an example of this would be to use standards from one data file for analysis on another data file)

MiraiBio also publishes the commercial software MasterPlex QT and the free web-based DNASIS SmartNote.

External links

• MiraiBio homepage
• MasterPlex ReaderFit
• Free demo, fully functional for 14 days
• Free online version of MasterPlex ReaderFit
• MasterPlex ReaderFit online resources