Biology:UPF3B
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Short description: Protein-coding gene in the species Homo sapiens
 Generic protein structure example |
Regulator of nonsense transcripts 3B is a protein that in humans is encoded by the UPF3B gene.[1][2][3]
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.[3]
Interactions
UPF3B has been shown to interact with UPF2[2][4][5][6] and UPF1.[2][5][7]
References
- ↑ "Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4)". Mol Cell Biol 21 (1): 209–23. Jan 2001. doi:10.1128/MCB.21.1.209-223.2001. PMID 11113196.
- ↑ 2.0 2.1 2.2 "Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon". Cell 103 (7): 1121–31. Feb 2001. doi:10.1016/S0092-8674(00)00214-2. PMID 11163187.
- ↑ 3.0 3.1 "Entrez Gene: UPF3B UPF3 regulator of nonsense transcripts homolog B (yeast)". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=65109.
- ↑ Lehner, Ben; Sanderson Christopher M (Jul 2004). "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. ISSN 1088-9051. PMID 15231747.
- ↑ 5.0 5.1 Lejeune, Fabrice; Li Xiaojie; Maquat Lynne E (Sep 2003). "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities". Mol. Cell 12 (3): 675–87. doi:10.1016/S1097-2765(03)00349-6. ISSN 1097-2765. PMID 14527413.
- ↑ Lykke-Andersen, Jens (Dec 2002). "Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay". Mol. Cell. Biol. 22 (23): 8114–21. doi:10.1128/MCB.22.23.8114-8121.2002. ISSN 0270-7306. PMID 12417715.
- ↑ Schell, Thomas; Köcher Thomas; Wilm Matthias; Seraphin Bertrand; Kulozik Andreas E; Hentze Matthias W (Aug 2003). "Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells". Biochem. J. 373 (Pt 3): 775–83. doi:10.1042/BJ20021920. ISSN 0264-6021. PMID 12723973.
Further reading
- "Purification of CpG islands using a methylated DNA binding column". Nat. Genet. 6 (3): 236–44. 1994. doi:10.1038/ng0394-236. PMID 8012384.
- "The nuclear cap-binding complex is a novel target of growth factor receptor-coupled signal transduction". J. Biol. Chem. 274 (7): 4166–73. 1999. doi:10.1074/jbc.274.7.4166. PMID 9933612.
- "Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex". Science 293 (5536): 1832–6. 2001. doi:10.1126/science.1062829. PMID 11546873. Bibcode: 2001Sci...293.1832K.
- "Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1". Science 293 (5536): 1836–9. 2001. doi:10.1126/science.1062786. PMID 11546874. Bibcode: 2001Sci...293.1836L.
- "The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling". EMBO J. 21 (13): 3536–45. 2002. doi:10.1093/emboj/cdf345. PMID 12093754.
- Lykke-Andersen J (2003). "Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay". Mol. Cell. Biol. 22 (23): 8114–21. doi:10.1128/MCB.22.23.8114-8121.2002. PMID 12417715.
- "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. 2003. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M.
- "Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1". RNA 9 (1): 77–87. 2003. doi:10.1261/rna.2137903. PMID 12554878.
- "Y14 and hUpf3b form an NMD-activating complex". Mol. Cell 11 (4): 939–49. 2003. doi:10.1016/S1097-2765(03)00142-4. PMID 12718880.
- "Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells". Biochem. J. 373 (Pt 3): 775–83. 2003. doi:10.1042/BJ20021920. PMID 12723973.
- "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities". Mol. Cell 12 (3): 675–87. 2003. doi:10.1016/S1097-2765(03)00349-6. PMID 14527413.
- "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. 2004. doi:10.1038/ng1285. PMID 14702039.
- "The structural basis for the interaction between nonsense-mediated mRNA decay factors UPF2 and UPF3". Nat. Struct. Mol. Biol. 11 (4): 330–7. 2004. doi:10.1038/nsmb741. PMID 15004547.
- "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. 2004. doi:10.1101/gr.2122004. PMID 15231747.
- "Nucleolar proteome dynamics". Nature 433 (7021): 77–83. 2005. doi:10.1038/nature03207. PMID 15635413. Bibcode: 2005Natur.433...77A.
- "Mammalian Staufen1 recruits Upf1 to specific mRNA 3'UTRs so as to elicit mRNA decay". Cell 120 (2): 195–208. 2005. doi:10.1016/j.cell.2004.11.050. PMID 15680326.
- "Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements". Mol. Cell 20 (1): 65–75. 2005. doi:10.1016/j.molcel.2005.08.012. PMID 16209946.
- "Functions of hUpf3a and hUpf3b in nonsense-mediated mRNA decay and translation". RNA 12 (6): 1015–22. 2006. doi:10.1261/rna.12506. PMID 16601204.
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