Biology:RBM9
 Generic protein structure example |
RNA binding motif protein 9 (RBM9), also known as Rbfox2, is a protein which in humans is encoded by the RBM9 gene.[1]
Function
Rbfox2 is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative splicing in the nervous system and other cell types. Rbfox2 and the related protein Rbfox1 bind to conserved (U)GCAUG RNA motifs in the introns adjacent to many alternatively spliced exons and promotes inclusion or exclusion of the alternative exon in mature transcripts.[2][3] The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.[1]
Rbfox2, as determined by CLIP-seq, binds near alternatively spliced exons and regulates the inclusion or exclusion of exons during alternative splicing by binding in introns either downstream (inclusion) or upstream (exon skipping) of exons. Its presence is important for stem cell survival and knockdowns of Rbfox2 activate markers for apoptosis.[4]
See also
References
- ↑ 1.0 1.1 "Entrez Gene: RBM9 RNA binding motif protein 9". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=23543.
- ↑ Jin, Y. (2003-02-17). "A vertebrate RNA-binding protein Fox-1 regulates tissue-specific splicing via the pentanucleotide GCAUG". The EMBO Journal 22 (4): 905–912. doi:10.1093/emboj/cdg089. ISSN 1460-2075. PMID 12574126.
- ↑ Ponthier, Julie L.; Schluepen, Christina; Chen, Weiguo; Lersch, Robert A.; Gee, Sherry L.; Hou, Victor C.; Lo, Annie J.; Short, Sarah A. et al. (2006-03-14). "Fox-2 Splicing Factor Binds to a Conserved Intron Motif to Promote Inclusion of Protein 4.1R Alternative Exon 16". Journal of Biological Chemistry 281 (18): 12468–12474. doi:10.1074/jbc.m511556200. ISSN 0021-9258. PMID 16537540.
- ↑ "An RNA code for the FOX2 splicing regulator revealed by mapping RNA-protein interactions in stem cells". Nature Structural & Molecular Biology 16 (2): 130–137. February 2009. doi:10.1038/nsmb.1545. PMID 19136955.
Further reading
- Dunham I; Shimizu N; Roe BA et al. (1999). "The DNA sequence of human chromosome 22". Nature 402 (6761): 489–495. doi:10.1038/990031. PMID 10591208. Bibcode: 1999Natur.402..489D.
- Lieberman AP; Friedlich DL; Harmison G et al. (2001). "Androgens regulate the mammalian homologues of invertebrate sex determination genes tra-2 and fox-1". Biochem. Biophys. Res. Commun. 282 (2): 499–506. doi:10.1006/bbrc.2001.4617. PMID 11401487. https://semanticscholar.org/paper/c4bbff99f2bb166f88b155eaa85ffff5d19b62a1.
- "A negative coregulator for the human ER". Mol. Endocrinol. 16 (3): 459–468. 2002. doi:10.1210/me.16.3.459. PMID 11875103.
- Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M.
- Collins JE; Goward ME; Cole CG et al. (2003). "Reevaluating human gene annotation: a second-generation analysis of chromosome 22". Genome Res. 13 (1): 27–36. doi:10.1101/gr.695703. PMID 12529303.
- Ota T; Suzuki Y; Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–45. doi:10.1038/ng1285. PMID 14702039.
- Collins JE; Wright CL; Edwards CA et al. (2005). "A genome annotation-driven approach to cloning the human ORFeome". Genome Biol. 5 (10): R84. doi:10.1186/gb-2004-5-10-r84. PMID 15461802.
- Gerhard DS; Wagner L; Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMID 15489334.
- Rual JF; Venkatesan K; Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R.
- Underwood JG; Boutz PL; Dougherty JD et al. (2005). "Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals". Mol. Cell. Biol. 25 (22): 10005–10016. doi:10.1128/MCB.25.22.10005-10016.2005. PMID 16260614.
- Kimura K; Wakamatsu A; Suzuki Y et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMID 16344560.
- Ponthier JL; Schluepen C; Chen W et al. (2006). "Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16". J. Biol. Chem. 281 (18): 12468–12474. doi:10.1074/jbc.M511556200. PMID 16537540.
- Lim J; Hao T; Shaw C et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell 125 (4): 801–814. doi:10.1016/j.cell.2006.03.032. PMID 16713569.
External links
- Overview of all the structural information available in the PDB for UniProt: O43251 (RNA binding protein fox-1 homolog 2) at the PDBe-KB.
PDB gallery | |
|---|---|
|
 |
