Biology:RNA-binding protein

From HandWiki
Short description: Type of protein
Not to be confused with retinol binding proteins, also abbreviated as RBPs.

RNA-binding proteins (often abbreviated as RBPs) are proteins that bind to the double or single stranded RNA[1] in cells and participate in forming ribonucleoprotein complexes. RBPs contain various structural motifs, such as RNA recognition motif (RRM), dsRNA binding domain, zinc finger and others.[2][3] They are cytoplasmic and nuclear proteins. However, since most mature RNA is exported from the nucleus relatively quickly, most RBPs in the nucleus exist as complexes of protein and pre-mRNA called heterogeneous ribonucleoprotein particles (hnRNPs). RBPs have crucial roles in various cellular processes such as: cellular function, transport and localization. They especially play a major role in post-transcriptional control of RNAs, such as: splicing, polyadenylation, mRNA stabilization, mRNA localization and translation. Eukaryotic cells express diverse RBPs with unique RNA-binding activity and protein–protein interaction. According to the Eukaryotic RBP Database (EuRBPDB), there are 2961 genes encoding RBPs in humans. During evolution, the diversity of RBPs greatly increased with the increase in the number of introns. Diversity enabled eukaryotic cells to utilize RNA exons in various arrangements, giving rise to a unique RNP (ribonucleoprotein) for each RNA. Although RBPs have a crucial role in post-transcriptional regulation in gene expression, relatively few RBPs have been studied systematically.It has now become clear that RNA–RBP interactions play important roles in many biological processes among organisms.[4][5][6]

Structure

Many RBPs have modular structures and are composed of multiple repeats of just a few specific basic domains that often have limited sequences. Different RBPs contain these sequences arranged in varying combinations. A specific protein's recognition of a specific RNA has evolved through the rearrangement of these few basic domains. Each basic domain recognizes RNA, but many of these proteins require multiple copies of one of the many common domains to function.[2]

Diversity

As nuclear RNA emerges from RNA polymerase, RNA transcripts are immediately covered with RNA-binding proteins that regulate every aspect of RNA metabolism and function including RNA biogenesis, maturation, transport, cellular localization and stability. All RBPs bind RNA, however they do so with different RNA-sequence specificities and affinities, which allows the RBPs to be as diverse as their targets and functions.[5] These targets include mRNA, which codes for proteins, as well as a number of functional non-coding RNAs. NcRNAs almost always function as ribonucleoprotein complexes and not as naked RNAs. These non-coding RNAs include microRNAs, small interfering RNAs (siRNA), as well as spliceosomal small nuclear RNAs (snRNA).[7]

Function

RNA processing and modification

Alternative splicing

Alternative splicing is a mechanism by which different forms of mature mRNAs (messengers RNAs) are generated from the same gene. It is a regulatory mechanism by which variations in the incorporation of the exons into mRNA leads to the production of more than one related protein, thus expanding possible genomic outputs. RBPs function extensively in the regulation of this process. Some binding proteins such as neuronal specific RNA-binding proteins, namely NOVA1, control the alternative splicing of a subset of hnRNA by recognizing and binding to a specific sequence in the RNA (YCAY where Y indicates pyrimidine, U or C).[5] These proteins then recruit splicesomal proteins to this target site. SR proteins are also well known for their role in alternative splicing through the recruitment of snRNPs that form the splicesome, namely U1 snRNP and U2AF snRNP. However, RBPs are also part of the splicesome itself. The splicesome is a complex of snRNA and protein subunits and acts as the mechanical agent that removes introns and ligates the flanking exons.[7] Other than core splicesome complex, RBPs also bind to the sites of Cis-acting RNA elements that influence exons inclusion or exclusion during splicing. These sites are referred to as exonic splicing enhancers (ESEs), exonic splicing silencers (ESSs), intronic splicing enhancers (ISEs) and intronic splicing silencers (ISSs) and depending on their location of binding, RBPs work as splicing silencers or enhancers.[8]

RNA editing

ADAR Protein.
ADAR : an RNA binding protein involved in RNA editing events.

The most extensively studied form of RNA editing involves the ADAR protein. This protein functions through post-transcriptional modification of mRNA transcripts by changing the nucleotide content of the RNA. This is done through the conversion of adenosine to inosine in an enzymatic reaction catalyzed by ADAR. This process effectively changes the RNA sequence from that encoded by the genome and extends the diversity of the gene products. The majority of RNA editing occurs on non-coding regions of RNA; however, some protein-encoding RNA transcripts have been shown to be subject to editing resulting in a difference in their protein's amino acid sequence. An example of this is the glutamate receptor mRNA where glutamine is converted to arginine leading to a change in the functionality of the protein.[5]

Polyadenylation

Main page: Biology:Polyadenylation

Polyadenylation is the addition of a "tail" of adenylate residues to an RNA transcript about 20 bases downstream of the AAUAAA sequence within the three prime untranslated region. Polyadenylation of mRNA has a strong effect on its nuclear transport, translation efficiency, and stability. All of these as well as the process of polyadenylation depend on binding of specific RBPs. All eukaryotic mRNAs with few exceptions are processed to receive 3' poly (A) tails of about 200 nucleotides. One of the necessary protein complexes in this process is CPSF. CPSF binds to the 3' tail (AAUAAA) sequence and together with another protein called poly(A)-binding protein, recruits and stimulates the activity of poly(A) polymerase. Poly(A) polymerase is inactive on its own and requires the binding of these other proteins to function properly.[5]

Export

After processing is complete, mRNA needs to be transported from the cell nucleus to cytoplasm. This is a three-step process involving the generation of a cargo-carrier complex in the nucleus followed by translocation of the complex through the nuclear pore complex and finally release of the cargo into cytoplasm. The carrier is then subsequently recycled. TAP/NXF1:p15 heterodimer is thought to be the key player in mRNA export. Over-expression of TAP in Xenopus laevis frogs increases the export of transcripts that are otherwise inefficiently exported. However TAP needs adaptor proteins because it is unable interact directly with mRNA. Aly/REF protein interacts and binds to the mRNA recruiting TAP.[5]

mRNA localization

mRNA localization is critical for regulation of gene expression by allowing spatially regulated protein production. Through mRNA localization proteins are translated in their intended target site of the cell. This is especially important during early development when rapid cell cleavages give different cells various combinations of mRNA which can then lead to drastically different cell fates. RBPs are critical in the localization of this mRNA that insures proteins are only translated in their intended regions. One of these proteins is ZBP1. ZBP1 binds to beta-actin mRNA at the site of transcription and moves with mRNA into the cytoplasm. It then localizes this mRNA to the lamella region of several asymmetric cell types where it can then be translated.[5] In 2008 it was proposed that FMRP was involved in the stimulus-induced localization of several dendritic mRNAs in the neuronal dendrites of cultured hippocampal neurons.[9] More recent studies of FMRP-bound RNAs present in microdissected dendrites of CA1 hippocampal neurons revealed no changes in localization in wild type versus FMRP-null mouse brains.[10]

Translation

Translational regulation provides a rapid mechanism to control gene expression. Rather than controlling gene expression at the transcriptional level, mRNA is already transcribed but the recruitment of ribosomes is controlled. This allows rapid generation of proteins when a signal activates translation. ZBP1 in addition to its role in the localization of B-actin mRNA is also involved in the translational repression of beta-actin mRNA by blocking translation initiation. ZBP1 must be removed from the mRNA to allow the ribosome to properly bind and translation to begin.[5]

Protein–RNA interactions

Diverse RNA contacts of RNA-binding proteins

RNA-binding proteins exhibit highly specific recognition of their RNA targets by recognizing their sequences, structures, motifs and RNA modifications.[11] Specific binding of the RNA-binding proteins allow them to distinguish their targets and regulate a variety of cellular functions via control of the generation, maturation, and lifespan of the RNA transcript. This interaction begins during transcription as some RBPs remain bound to RNA until degradation whereas others only transiently bind to RNA to regulate RNA splicing, processing, transport, and localization.[12] Cross-linking immunoprecipitation (CLIP) methods are used to stringently identify direct RNA binding sites of RNA-binding proteins in a variety of tissues and organisms. In this section, three classes of the most widely studied RNA-binding domains (RNA-recognition motif, double-stranded RNA-binding motif, zinc-finger motif) will be discussed.

RNA-recognition motif (RRM)

The RNA recognition motif, which is the most common RNA-binding motif, is a small protein domain of 75–85 amino acids that forms a four-stranded β-sheet against the two α-helices. This recognition motif exerts its role in numerous cellular functions, especially in mRNA/rRNA processing, splicing, translation regulation, RNA export, and RNA stability. Ten structures of an RRM have been identified through NMR spectroscopy and X-ray crystallography. These structures illustrate the intricacy of protein–RNA recognition of RRM as it entails RNA–RNA and protein–protein interactions in addition to protein–RNA interactions. Despite their complexity, all ten structures have some common features. All RRMs' main protein surfaces' four-stranded β-sheet was found to interact with the RNA, which usually contacts two or three nucleotides in a specific manner. In addition, strong RNA binding affinity and specificity towards variation are achieved through an interaction between the inter-domain linker and the RNA and between RRMs themselves. This plasticity of the RRM explains why RRM is the most abundant domain and why it plays an important role in various biological functions.[12]

Double-stranded RNA-binding motif

Double-stranded RNA-binding motif
PDB 2b7t EBI.jpg
dsRBD from rat ADAR2 protein (PDB: 2b7t​).
Identifiers
Symboldrrm
PfamPF14709
Pfam clanCL0196
InterProIPR014720
CATH1di2
SCOP21di2 / SCOPe / SUPFAM
Use the Pfam clan for the homologous superfamily.

The double-stranded RNA-binding motif (dsRM, dsRBD), a 70–75 amino-acid domain, plays a critical role in RNA processing, RNA localization, RNA interference, RNA editing, and translational repression. All three structures of the domain solved as of 2005 possess uniting features that explain how dsRMs only bind to dsRNA instead of dsDNA. The dsRMs were found to interact along the RNA duplex via both α-helices and β1-β2 loop. Moreover, all three dsRBM structures make contact with the sugar-phosphate backbone of the major groove and of one minor groove, which is mediated by the β1-β2 loop along with the N-terminus region of the alpha helix 2. This interaction is a unique adaptation for the shape of an RNA double helix as it involves 2'-hydroxyls and phosphate oxygen. Despite the common structural features among dsRBMs, they exhibit distinct chemical frameworks, which permits specificity for a variety for RNA structures including stem-loops, internal loops, bulges or helices containing mismatches.[12]

Zinc fingers

Zinc finger.
"Zinc finger" : Cartoon representation of the zinc-finger motif of proteins. The zinc ion (green) is coordinated by two histidine and two cysteine amino acid residues.

CCHH-type zinc-finger domains are the most common DNA-binding domain within the eukaryotic genome. In order to attain high sequence-specific recognition of DNA, several zinc fingers are utilized in a modular fashion. Zinc fingers exhibit ββα protein fold in which a β-hairpin and a α-helix are joined via a Zn2+ ion. Furthermore, the interaction between protein side-chains of the α-helix with the DNA bases in the major groove allows for the DNA-sequence-specific recognition. Despite its wide recognition of DNA, there has been recent discoveries that zinc fingers also have the ability to recognize RNA. In addition to CCHH zinc fingers, CCCH zinc fingers were recently discovered to employ sequence-specific recognition of single-stranded RNA through an interaction between intermolecular hydrogen bonds and Watson-Crick edges of the RNA bases. CCHH-type zinc fingers employ two methods of RNA binding. First, the zinc fingers exert non-specific interaction with the backbone of a double helix whereas the second mode allows zinc fingers to specifically recognize the individual bases that bulge out. Differing from the CCHH-type, the CCCH-type zinc finger displays another mode of RNA binding, in which single-stranded RNA is identified in a sequence-specific manner. Overall, zinc fingers can directly recognize DNA via binding to dsDNA sequence and RNA via binding to ssRNA sequence.[12]

Role in embryonic development

Caenorhabditis elegans.
Crawling C. elegans hermaphrodite worm

RNA-binding proteins' transcriptional and post-transcriptional regulation of RNA has a role in regulating the patterns of gene expression during development.[13] Extensive research on the nematode C. elegans has identified RNA-binding proteins as essential factors during germline and early embryonic development. Their specific function involves the development of somatic tissues (neurons, hypodermis, muscles and excretory cells) as well as providing timing cues for the developmental events. Nevertheless, it is exceptionally challenging to discover the mechanism behind RBPs' function in development due to the difficulty in identifying their RNA targets. This is because most RBPs usually have multiple RNA targets.[14] However, it is indisputable that RBPs exert a critical control in regulating developmental pathways in a concerted manner.

Germline development

In Drosophila melanogaster, Elav, Sxl and tra-2 are RNA-binding protein encoding genes that are critical in the early sex determination and the maintenance of the somatic sexual state.[15] These genes impose effects on the post-transcriptional level by regulating sex-specific splicing in Drosophila. Sxl exerts positive regulation of the feminizing gene tra to produce a functional tra mRNA in females. In C. elegans, RNA-binding proteins including FOG-1, MOG-1/-4/-5 and RNP-4 regulate germline and somatic sex determination. Furthermore, several RBPs such as GLD-1, GLD-3, DAZ-1, PGL-1 and OMA-1/-2 exert their regulatory functions during meiotic prophase progression, gametogenesis, and oocyte maturation.[14]

Somatic development

In addition to RBPs' functions in germline development, post-transcriptional control also plays a significant role in somatic development. Differing from RBPs that are involved in germline and early embryo development, RBPs functioning in somatic development regulate tissue-specific alternative splicing of the mRNA targets. For instance, MEC-8 and UNC-75 containing RRM domains localize to regions of hypodermis and nervous system, respectively.[14] Furthermore, another RRM-containing RBP, EXC-7, is revealed to localize in embryonic excretory canal cells and throughout the nervous system during somatic development.

Neuronal development

ZBP1 was shown to regulate dendritogenesis (dendrite formation) in hippocampal neurons.[16] Other RNA-binding proteins involved in dendrite formation are Pumilio and Nanos,[17] FMRP, CPEB and Staufen 1[18]

Role in cancer

RBPs are emerging to play a crucial role in tumor development.[19] Hundreds of RBPs are markedly dysregulated across human cancers and showed predominant downregulation in tumors related to normal tissues.[19] Many RBPs are differentially expressed in different cancer types for example KHDRBS1(Sam68),[20][21][22] ELAVL1(HuR),[23][24] FXR1[25] and UHMK1.[26] For some RBPs, the change in expression are related with Copy Number Variations (CNV), for example CNV gains of BYSL in colorectal cancer cells[19] and ESRP1, CELF3 in breast cancer, RBM24 in liver cancer, IGF2BP2, IGF2BP3 in lung cancer or CNV losses of KHDRBS2 in lung cancer.[27] Some expression changes are cause due to protein affecting mutations on these RBPs for example NSUN6, ZC3H13, ELAC1, RBMS3, and ZGPAT, SF3B1, SRSF2, RBM10, U2AF1, SF3B1, PPRC1, RBMXL1, HNRNPCL1 etc.[19][27][28][29][30] Several studies have related this change in expression of RBPs to aberrant alternative splicing in cancer.[27][31][32]

Current research

CIRBP.
"CIRBP" : Structure of the CIRBP protein.

As RNA-binding proteins exert significant control over numerous cellular functions, they have been a popular area of investigation for many researchers. Due to its importance in the biological field, numerous discoveries regarding RNA-binding proteins' potentials have been recently unveiled.[12] Recent development in experimental identification of RNA-binding proteins has extended the number of RNA-binding proteins significantly[33][34][35]

RNA-binding protein Sam68 controls the spatial and temporal compartmentalization of RNA metabolism to attain proper synaptic function in dendrites. Loss of Sam68 results in abnormal posttranscriptional regulation and ultimately leads to neurological disorders such as fragile X-associated tremor/ataxia syndrome. Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Therefore, Sam68 plays a critical role in regulating synapse number via control of postsynaptic β-actin mRNA metabolism.[36]

Beta-actin.
"Beta-actin" : Structure of the ACTB protein.

Neuron-specific CELF family RNA-binding protein UNC-75 specifically binds to the UUGUUGUGUUGU mRNA stretch via its three RNA recognition motifs for the exon 7a selection in C. elegans' neuronal cells. As exon 7a is skipped due to its weak splice sites in non-neuronal cells, UNC-75 was found to specifically activate splicing between exon 7a and exon 8 only in the neuronal cells.[37]

The cold inducible RNA binding protein CIRBP plays a role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. This research yielded potential implications for the association of disease states with inflammation.[38]

Serine-arginine family of RNA-binding protein Slr1 was found exert control on the polarized growth in Candida albicans. Slr1 mutations in mice results in decreased filamentation and reduces damage to epithelial and endothelial cells that leads to extended survival rate compared to the Slr1 wild-type strains. Therefore, this research reveals that SR-like protein Slr1 plays a role in instigating the hyphal formation and virulence in C. albicans.[39]

See also

External links

  • starBase platform: a platform for decoding binding sites of RNA binding proteins (RBPs) from large-scale CLIP-Seq (HITS-CLIP, PAR-CLIP, iCLIP, CLASH) datasets.
  • RBPDB database: a database of RNA binding proteins.
  • oRNAment: a database of putative RBP binding site instances in both coding and non-coding RNA in various species.
  • ATtRACt database: a database of RNA binding proteins and associated motifs.
  • SplicedAid-F: a database of hand -cureted human RNA binding proteins database.
  • RsiteDB: RNA binding site database
  • SPOT-Seq-RNA: Template-based prediction of RNA binding proteins and their complex structures.
  • SPOT-Struct-RNA: RNA binding proteins prediction from 3D structures.
  • ENCODE Project: A collection of genomic datasets (i.e. RNA Bind-n-seq, eCLIP, RBP targeted shRNA RNA-seq) for RBPs
  • RBP Image Database: Images showing the cellular localization of RBPs in cells
  • RBPSpot Software: A Deep-Learning based highly accurate software to detect RBP-RNA interaction. It also provides a module to build new RBP-RNA interaction models.

References

  1. ↑ RNA-Binding+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH)
  2. ↑ 2.0 2.1 "RNA-binding proteins: modular design for efficient function". Nature Reviews. Molecular Cell Biology 8 (6): 479–90. June 2007. doi:10.1038/nrm2178. PMID 17473849. 
  3. ↑ Liu, S; Li, B; Liang, Q; Liu, A; Qu, L; Yang, J (November 2020). "Classification and function of RNA-protein interactions.". Wiley Interdisciplinary Reviews. RNA 11 (6): e1601. doi:10.1002/wrna.1601. PMID 32488992. 
  4. ↑ "Diverse RNA-binding proteins interact with functionally related sets of RNAs, suggesting an extensive regulatory system". PLOS Biology 6 (10): e255. October 2008. doi:10.1371/journal.pbio.0060255. PMID 18959479. 
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 "RNA-binding proteins and post-transcriptional gene regulation". FEBS Letters 582 (14): 1977–86. June 2008. doi:10.1016/j.febslet.2008.03.004. PMID 18342629. 
  6. ↑ Liu, S; Li, B; Liang, Q; Liu, A; Qu, L; Yang, J (November 2020). "Classification and function of RNA-protein interactions.". Wiley Interdisciplinary Reviews. RNA 11 (6): e1601. doi:10.1002/wrna.1601. PMID 32488992. 
  7. ↑ 7.0 7.1 "Non-coding RNAs: lessons from the small nuclear and small nucleolar RNAs". Nature Reviews. Molecular Cell Biology 8 (3): 209–20. March 2007. doi:10.1038/nrm2124. PMID 17318225. 
  8. ↑ "Context-dependent control of alternative splicing by RNA-binding proteins". Nature Reviews. Genetics 15 (10): 689–701. October 2014. doi:10.1038/nrg3778. PMID 25112293. 
  9. ↑ "A direct role for FMRP in activity-dependent dendritic mRNA transport links filopodial-spine morphogenesis to fragile X syndrome". Developmental Cell 14 (6): 926–39. June 2008. doi:10.1016/j.devcel.2008.04.003. PMID 18539120. 
  10. ↑ Hale, Caryn R.; Sawicka, Kirsty; Mora, Kevin; Fak, John J.; Kang, Jin Joo; Cutrim, Paula; Cialowicz, Katarzyna; Carroll, Thomas S. et al. (2021-12-23). "FMRP regulates mRNAs encoding distinct functions in the cell body and dendrites of CA1 pyramidal neurons". eLife 10: e71892. doi:10.7554/eLife.71892. ISSN 2050-084X. PMID 34939924. 
  11. ↑ Liu, S; Li, B; Liang, Q; Liu, A; Qu, L; Yang, J (November 2020). "Classification and function of RNA-protein interactions.". Wiley Interdisciplinary Reviews. RNA 11 (6): e1601. doi:10.1002/wrna.1601. PMID 32488992. 
  12. ↑ 12.0 12.1 12.2 12.3 12.4 "RNA sequence- and shape-dependent recognition by proteins in the ribonucleoprotein particle". EMBO Reports 6 (1): 33–8. January 2005. doi:10.1038/sj.embor.7400325. PMID 15643449. 
  13. ↑ Appasani, Krishnarao (2008). MicroRNAs: From Basic Science to Disease Biology. Cambridge University Press. p. 485. ISBN 978-0-521-86598-2. https://books.google.com/books?id=H_W8rqYyc9MC&pg=PA485. Retrieved 12 May 2013. 
  14. ↑ 14.0 14.1 14.2 Lee, Min-Ho; Schedl, Tim (18 April 2006). "RNA-binding proteins". WormBook. WormBook. 1–13. doi:10.1895/wormbook.1.79.1. https://www.ncbi.nlm.nih.gov/books/NBK19709/. 
  15. ↑ "RNA-binding proteins as developmental regulators". Genes & Development 3 (4): 431–7. April 1989. doi:10.1101/gad.3.4.431. PMID 2470643. 
  16. ↑ "Zipcode binding protein 1 regulates the development of dendritic arbors in hippocampal neurons". The Journal of Neuroscience 31 (14): 5271–85. April 2011. doi:10.1523/JNEUROSCI.2387-10.2011. PMID 21471362. 
  17. ↑ "Nanos and Pumilio are essential for dendrite morphogenesis in Drosophila peripheral neurons". Current Biology 14 (4): 314–21. February 2004. doi:10.1016/j.cub.2004.01.052. PMID 14972682. 
  18. ↑ "A loss of function allele for murine Staufen1 leads to impairment of dendritic Staufen1-RNP delivery and dendritic spine morphogenesis". Proceedings of the National Academy of Sciences of the United States of America 105 (42): 16374–9. October 2008. doi:10.1073/pnas.0804583105. PMID 18922781. Bibcode: 2008PNAS..10516374V. 
  19. ↑ 19.0 19.1 19.2 19.3 "Comprehensive Genomic Characterization of RNA-Binding Proteins across Human Cancers". Cell Reports 22 (1): 286–298. January 2018. doi:10.1016/j.celrep.2017.12.035. PMID 29298429. 
  20. ↑ "The RNA-binding protein Sam68 is a multifunctional player in human cancer". Endocrine-Related Cancer 18 (4): R91–R102. August 2011. doi:10.1530/ERC-11-0041. PMID 21565971. http://erc.endocrinology-journals.org/content/18/4/R91. 
  21. ↑ "High expression level and nuclear localization of Sam68 are associated with progression and poor prognosis in colorectal cancer". BMC Gastroenterology 13: 126. August 2013. doi:10.1186/1471-230X-13-126. PMID 23937454. 
  22. ↑ "SAM68: Signal Transduction and RNA Metabolism in Human Cancer". BioMed Research International 2015: 528954. 26 July 2015. doi:10.1155/2015/528954. PMID 26273626. 
  23. ↑ "Posttranscriptional regulation of cancer traits by HuR". Wiley Interdisciplinary Reviews: RNA 1 (2): 214–29. 1 September 2010. doi:10.1002/wrna.4. PMID 21935886. 
  24. ↑ "Multiple functions of the RNA-binding protein HuR in cancer progression, treatment responses and prognosis". International Journal of Molecular Sciences 14 (5): 10015–41. May 2013. doi:10.3390/ijms140510015. PMID 23665903. 
  25. ↑ "The RNA binding protein FXR1 is a new driver in the 3q26-29 amplicon and predicts poor prognosis in human cancers". Proceedings of the National Academy of Sciences of the United States of America 112 (11): 3469–74. March 2015. doi:10.1073/pnas.1421975112. PMID 25733852. Bibcode: 2015PNAS..112.3469Q. 
  26. ↑ Feng, Xing; Ma, Dong; Zhao, Jiabao; Song, Yongxi; Zhu, Yuekun; Zhou, Qingxin; Ma, Fei; Liu, Xing et al. (2020-03-02). "UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism". The EMBO Journal 39 (5): e102541. doi:10.15252/embj.2019102541. ISSN 1460-2075. PMID 31975428. 
  27. ↑ 27.0 27.1 27.2 "Large-scale analysis of genome and transcriptome alterations in multiple tumors unveils novel cancer-relevant splicing networks". Genome Research 26 (6): 732–44. June 2016. doi:10.1101/gr.199935.115. PMID 27197215. 
  28. ↑ "Frequent pathway mutations of splicing machinery in myelodysplasia". Nature 478 (7367): 64–9. September 2011. doi:10.1038/nature10496. PMID 21909114. Bibcode: 2011Natur.478...64Y. 
  29. ↑ "Mapping the hallmarks of lung adenocarcinoma with massively parallel sequencing" (in en). Cell 150 (6): 1107–20. September 2012. doi:10.1016/j.cell.2012.08.029. PMID 22980975. 
  30. ↑ "Whole-genome analysis informs breast cancer response to aromatase inhibition". Nature 486 (7403): 353–60. June 2012. doi:10.1038/nature11143. PMID 22722193. Bibcode: 2012Natur.486..353E. 
  31. ↑ "Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged". Genes & Development 24 (21): 2343–64. November 2010. doi:10.1101/gad.1973010. PMID 21041405. 
  32. ↑ "RNA-Binding Proteins: Splicing Factors and Disease". Biomolecules 5 (2): 893–909. May 2015. doi:10.3390/biom5020893. PMID 25985083. 
  33. ↑ "Serial interactome capture of the human cell nucleus". Nature Communications 7: 11212. April 2016. doi:10.1038/ncomms11212. PMID 27040163. Bibcode: 2016NatCo...711212C. 
  34. ↑ "Insights into RNA biology from an atlas of mammalian mRNA-binding proteins". Cell 149 (6): 1393–406. June 2012. doi:10.1016/j.cell.2012.04.031. PMID 22658674. 
  35. ↑ "The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts". Molecular Cell 46 (5): 674–90. June 2012. doi:10.1016/j.molcel.2012.05.021. PMID 22681889. 
  36. ↑ "RNA-binding protein Sam68 controls synapse number and local β-actin mRNA metabolism in dendrites". Proceedings of the National Academy of Sciences of the United States of America 110 (8): 3125–30. February 2013. doi:10.1073/pnas.1209811110. PMID 23382180. Bibcode: 2013PNAS..110.3125K. 
  37. ↑ Blumenthal, Tom, ed (2013). "CELF family RNA-binding protein UNC-75 regulates two sets of mutually exclusive exons of the unc-32 gene in neuron-specific manners in Caenorhabditis elegans". PLOS Genetics 9 (2): e1003337. doi:10.1371/journal.pgen.1003337. PMID 23468662. 
  38. ↑ Gallouzi, Imed Eddine, ed (2013). "NF-κB-dependent role for cold-inducible RNA binding protein in regulating interleukin 1β". PLOS ONE 8 (2): e57426. doi:10.1371/journal.pone.0057426. PMID 23437386. Bibcode: 2013PLoSO...857426B. 
  39. ↑ "SR-like RNA-binding protein Slr1 affects Candida albicans filamentation and virulence". Infection and Immunity 81 (4): 1267–76. April 2013. doi:10.1128/IAI.00864-12. PMID 23381995. 



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