Biography:Norbert Perrimon

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Short description: French-American geneticist
Norbert Perrimon
Born (1958-10-24) October 24, 1958 (age 66) [1]
Bosguérard-de-Marcouville[1]
NationalityFrench
CitizenshipFrance, United States
Alma materUniversity of Paris
Known forGAL4/UAS system
AwardsGeorge W. Beadle Award (2004)[2]
Scientific career
Institutions
ThesisAnalyse Clonale de Mutations en Lignee Germinale chez la Drosophile (1983)
Academic advisorsMadeleine Gans
Notable studentsSara Cherry
Website

Norbert Perrimon is a French geneticist and developmental biologist. He is the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School, an Investigator at the Howard Hughes Medical Institute, and an Associate of the Broad Institute. He is known for developing a number of techniques for used in genetic research with Drosophila melanogaster, as well as specific substantive contributions to signal transduction, developmental biology and physiology.

Education

Perrimon was born in 1958 in Bosguérard-de-Marcouville, France. He earned his undergraduate degree (Maitrise of Biochemistry) at the University of Paris VI, in 1981, then completed his doctorate in 1983 with Madeleine Gans, also at the University of Paris.

Career

From 1983 to 1986 Perrimon was a postdoctoral researcher with Anthony Mahowald[3][4][5][6] at Case Western Reserve University, and in 1986 at the age of 27 he accepted an appointment as faculty at Harvard Medical School. He is currently the James Stillman Professor of Developmental Biology in the Department of Genetics at Harvard Medical School. He has been an Investigator of the Howard Hughes Medical Institute since 1986.[7]

Research

Perrimon's group developed many methods that have significantly improved the Drosophila toolbox. Perrimon co-developed the GAL4/UAS system method with Andrea Brand to control gene expression in Drosophila.[8] This method has been described as “a fly geneticist's Swiss army knife”[9] and is widely used in Drosophila genetics. Together with Tze-bin Chou, he developed the FLP-FRT DFS method to generate germline mosaics, a method that allowed the large-scale characterization of the maternal effect of zygotic lethal mutations.[10][11][12] He developed and improved methods in vivo RNAi with Janquan Ni.[13][14][15] His lab has pioneered high-throughput whole-genome RNAi screening to interrogate systematically the function of all fly genes in various cell-based assays.[16][17][18][19][20][21][22] With Ram Viswanatha, he developed CRISPR/Cas9 pooled screens in Drosophila cells to facilitate large-scale screen in Drosophila and other arthropod cell lines.[23] The approach is particularly powerful to identify the mechanism of entry of toxins.[24]

In 2003 he created the Drosophila RNAi Screening Center at Harvard Medical School and in 2008, he initiated the Transgenic RNAi Project to generate transgenic RNAi lines for the community using optimized shRNA vectors that his lab developed.

Awards and honors

Perrimon was elected to the United States National Academy of Sciences in April 2013,[18][25] after naturalizing as an American citizen.

  • Lucille P. Markey Scholar in Biomedical Sciences, 1985.[26]
  • Investigator, Howard Hughes Medical Institute, 1986–present
  • Chaire d’Etat. College de France. Paris, 2003
  • George W. Beadle Medal, Genetics Society of America, 2004[2]
  • RNAi Innovator Award, 2009[27]
  • Fellow of the American Academy of Arts and Sciences, 2008[28]
  • Fellow of the American Association for the Advancement of Science, 2009
  • Associate member of the European Molecular Biology Organization (EMBO) 2011
  • Fellow of the United States National Academy of Sciences, 2013

References

  1. ↑ 1.0 1.1 "Archived copy". http://perrimon.med.harvard.edu/Perrimon_2014_LongCV.doc. 
  2. ↑ 2.0 2.1 Schüpbach, T. (2004). "The 2004 George W. Beadle Medal". Genetics 166 (2): 649–650. doi:10.1534/genetics.166.2.649. PMID 15020455. 
  3. ↑ Perrimon, N; Engstrom, L; Mahowald, A. P. (1985). "Developmental genetics of the 2C-D region of the Drosophila X chromosome". Genetics 111 (1): 23–41. doi:10.1093/genetics/111.1.23. PMID 3928431. 
  4. ↑ Perrimon, N; Mohler, D; Engstrom, L; Mahowald, A. P. (1986). "X-linked female-sterile loci in Drosophila melanogaster". Genetics 113 (3): 695–712. doi:10.1093/genetics/113.3.695. PMID 3089870. 
  5. ↑ Perrimon, N; Mahowald, A. P. (1986). "L(1)hopscotch, A larval-pupal zygotic lethal with a specific maternal effect on segmentation in Drosophila". Developmental Biology 118 (1): 28–41. doi:10.1016/0012-1606(86)90070-9. PMID 3095163. 
  6. ↑ Perrimon, N; Mahowald, A. P. (1987). "Multiple functions of segment polarity genes in Drosophila". Developmental Biology 119 (2): 587–600. doi:10.1016/0012-1606(87)90061-3. PMID 3803719. 
  7. ↑ "Norbert Perrimon, PhD | HHMI.org". HHMI.org. http://www.hhmi.org/scientists/norbert-perrimon. 
  8. ↑ Brand, A. H.; Perrimon, N. (1993). "Targeted gene expression as a means of altering cell fates and generating dominant phenotypes". Development 118 (2): 401–415. doi:10.1242/dev.118.2.401. PMID 8223268. http://dev.biologists.org/content/118/2/401.long. 
  9. ↑ Shetty, P. (2008). "Molecular biologist Andrea Brand: encouraging women in science". The Lancet 371 (9617): 979. doi:10.1016/S0140-6736(08)60439-0. PMID 18358916. 
  10. ↑ Chou, T. B.; Perrimon, N. (1992-07-01). "Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila." (in en). Genetics 131 (3): 643–653. doi:10.1093/genetics/131.3.643. ISSN 0016-6731. PMID 1628809. PMC 1205036. http://www.genetics.org/content/131/3/643. 
  11. ↑ Chou, T. B.; Noll, E.; Perrimon, N. (1993-12-01). "Autosomal P[ovoD1 dominant female-sterile insertions in Drosophila and their use in generating germ-line chimeras"] (in en). Development 119 (4): 1359–1369. doi:10.1242/dev.119.4.1359. ISSN 0950-1991. PMID 8306893. http://dev.biologists.org/content/119/4/1359. 
  12. ↑ Chou, Tze-bin; Perrimon, Norbert (1996-12-01). "The Autosomal FLP-DFS Technique for Generating Germline Mosaics in Drosophila melanogaster" (in en). Genetics 144 (4): 1673–1679. doi:10.1093/genetics/144.4.1673. ISSN 0016-6731. PMID 8978054. PMC 1207718. http://www.genetics.org/content/144/4/1673. 
  13. ↑ Ni, Jian-Quan; Markstein, Michele; Binari, Richard; Pfeiffer, Barret; Liu, Lu-Ping; Villalta, Christians; Booker, Matthew; Perkins, Lizabeth et al. (2008-01-01). "Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster" (in en). Nature Methods 5 (1): 49–51. doi:10.1038/nmeth1146. ISSN 1548-7091. PMID 18084299. 
  14. ↑ Ni, Jian-Quan; Liu, Lu-Ping; Binari, Richard; Hardy, Robert; Shim, Hye-Seok; Cavallaro, Amanda; Booker, Matthew; Pfeiffer, Barret D. et al. (2009-08-01). "A Drosophila resource of transgenic RNAi lines for neurogenetics". Genetics 182 (4): 1089–1100. doi:10.1534/genetics.109.103630. ISSN 1943-2631. PMID 19487563. 
  15. ↑ Ni, Jian-Quan; Zhou, Rui; Czech, Benjamin; Liu, Lu-Ping; Holderbaum, Laura; Yang-Zhou, Donghui; Shim, Hye-Seok; Tao, Rong et al. (2011-05-01). "A genome-scale shRNA resource for transgenic RNAi in Drosophila". Nature Methods 8 (5): 405–407. doi:10.1038/nmeth.1592. ISSN 1548-7105. PMID 21460824. 
  16. ↑ Boutros, Michael; Kiger, Amy A.; Armknecht, Susan; Kerr, Kim; Hild, Marc; Koch, Britta; Haas, Stefan A.; Paro, Renato et al. (2004-02-06). "Genome-wide RNAi analysis of growth and viability in Drosophila cells". Science 303 (5659): 832–835. doi:10.1126/science.1091266. ISSN 1095-9203. PMID 14764878. https://pubmed.ncbi.nlm.nih.gov/14764878. 
  17. ↑ Perrimon, N. (2005). "Norbert Perrimon". Current Biology 15 (13): R481–R482. doi:10.1016/j.cub.2005.06.050. PMID 16059997. 
  18. ↑ 18.0 18.1 Ravindran, S (2014). "Profile of Norbert Perrimon". Proceedings of the National Academy of Sciences 111 (21): 7501–2. doi:10.1073/pnas.1406464111. PMID 24778217. Bibcode: 2014PNAS..111.7501R. 
  19. ↑ Perrimon, N; Gans, M (1983). "Clonal analysis of the tissue specificity of recessive female-sterile mutations of Drosophila melanogaster using a dominant female-sterile mutation Fs(1)K1237". Developmental Biology 100 (2): 365–73. doi:10.1016/0012-1606(83)90231-2. PMID 6418585. 
  20. ↑ Perrimon, N (1984). "Clonal Analysis of Dominant Female-Sterile, Germline-Dependent Mutations in DROSOPHILA MELANOGASTER". Genetics 108 (4): 927–39. doi:10.1093/genetics/108.4.927. PMID 17246244. 
  21. ↑ White, R. A.; Perrimon, N; Gehring, W. J. (1984). "Differentiation markers in the Drosophila ovary". Journal of Embryology and Experimental Morphology 84: 275–86. PMID 6442733. 
  22. ↑ Perrimon, N.; Engstrom, L.; Mahowald, A. P. (1984). "The effects of zygotic lethal mutations on female germ-line functions in Drosophila". Developmental Biology 105 (2): 404–414. doi:10.1016/0012-1606(84)90297-5. PMID 6479445. 
  23. ↑ Viswanatha, Raghuvir; Li, Zhongchi; Hu, Yanhui; Perrimon, Norbert (2018-07-27). "Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells". eLife 7: e36333. doi:10.7554/eLife.36333. ISSN 2050-084X. PMID 30051818. 
  24. ↑ Xu, Ying; Viswanatha, Raghuvir; Sitsel, Oleg; Roderer, Daniel; Zhao, Haifang; Ashwood, Christopher; Voelcker, Cecilia; Tian, Songhai et al. (October 2022). "CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor". Nature 610 (7931): 349–355. doi:10.1038/s41586-022-05250-7. ISSN 1476-4687. PMID 36171290. 
  25. ↑ "April 30, 2013, NAS Election", National Academy of Sciences (last visited May 3, 2013).
  26. ↑ Teltsch, Kathleen (1985-02-10). "16 ARE GIVEN MARKEY RESEARCH SCHOLARSHIPS" (in en-US). The New York Times. ISSN 0362-4331. https://www.nytimes.com/1985/02/10/us/16-are-given-markey-research-scholarships.html. 
  27. ↑ "Innovator Award Recipients". GeneExpression Systems. http://www.expressgenes.com/innovator-pix.htm. 
  28. ↑ "Member Directory | American Academy of Arts and Sciences". https://www.amacad.org/directory?search_api_fulltext=norbert%20perrimon&field_class_section=All&field_class_section_1=All&field_deceased=All&sort_bef_combine=search_api_relevance_DESC. 



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