Biology:Oocyte selection
Oocyte selection is a procedure that is performed prior to in vitro fertilization, in order to use oocytes with maximal chances of resulting in pregnancy. In contrast, embryo selection takes place after fertilization.
Techniques
Chromosomal evaluation may be performed. Embryos from rescued in vitro-matured metaphase II (IVM-MII) oocytes show significantly higher fertilization rates and more blastomeres per embryo compared with those from arrested metaphase I (MI) oocytes (58.5% vs. 43.9% and 5.7 vs. 5.0, respectively).[1]
Also, morphological features of the oocyte that can be obtained by standard light or polarized light microscopy. However, there is no clear tendency in recent publications to a general increase in predictive value of morphological features.[2] Suggested techniques include zona pellucida imaging, which can detect differences in birefringence between eggs, which is a predictor of compaction, blastulation and pregnancy.[3]
Potentially, polar body biopsy may be used for molecular analysis, and can be used for preimplantation genetic screening.[4]
References
- ↑ "The cytogenetic constitution of embryos derived from immature (metaphase I) oocytes obtained after ovarian hyperstimulation". Fertil. Steril. 94 (3): 971–978. June 2009. doi:10.1016/j.fertnstert.2009.04.035. PMID 19505687.
- ↑ Rienzi, L.; Vajta, G.; Ubaldi, F. (2010). "Predictive value of oocyte morphology in human IVF: a systematic review of the literature". Human Reproduction Update 17 (1): 34–45. doi:10.1093/humupd/dmq029. PMID 20639518.
- ↑ "Automatic user-independent zona pellucida imaging at the oocyte stage allows for the prediction of preimplantation development". Fertil. Steril. 94 (3): 913–920. May 2009. doi:10.1016/j.fertnstert.2009.03.106. PMID 19439291.
- ↑ Jiao, Ze-Xu; Woodruff, Teresa K. (2013). "Detection and quantification of maternal-effect gene transcripts in mouse second polar bodies: potential markers of embryo developmental competence". Fertility and Sterility 99 (7): 2055–2061. doi:10.1016/j.fertnstert.2013.02.003. ISSN 0015-0282. PMID 23465709.
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