Biology:MicX sRNA
| MicX Vibrio cholerae sRNA | |
|---|---|
 Secondary structure of MicX sRNA. | |
| Identifiers | |
| Symbol | MicX |
| Rfam | RF01808 |
| Other data | |
| RNA type | sRNA |
| Domain(s) | Vibrio cholerae |
| PDB structures | PDBe |
MicX sRNA (formerly known as A10)[1] is a small non-coding RNA found in Vibrio cholerae.[2] It was given the name MicX as it has a similar function to MicA, MicC and MicF in E. coli.[3] MicX sRNA negatively regulates an outer membrane protein (coded for by VC0972) and also a component of an ABC transporter (gene VC0620).[4] These interactions were predicted and then confirmed using a DNA microarray.[2]
MicX was identified through a bioinformatics screen of V. cholerae having been previously predicted.[1] Levels of transcription of this sRNA were compared under several conditions: it was found to be expressed on all tested mediums; richer mediums slightly reduced transcription; repression of certain sigma factors (δS and δE) did not change transcription but it was dramatically reduced in the absence of Hfq protein.[2] This observation is in accordance with other sRNA expression patterns.[5][6]
The MicX RNA gene overlaps with VCA0943 - a gene coding for a maltose transporter permease - but the ribonuclease RNAase E processes the MicX transcript to create an active and stable form containing only the VCA0943 3′ UTR.[2]
See also
References
- ↑ 1.0 1.1 "sRNAPredict: an integrative computational approach to identify sRNAs in bacterial genomes". Nucleic Acids Research 33 (13): 4096–4105. 2005. doi:10.1093/nar/gki715. PMID 16049021.
- ↑ 2.0 2.1 2.2 2.3 "RNase E-dependent processing stabilizes MicX, a Vibrio cholerae sRNA". Molecular Microbiology 65 (2): 373–385. July 2007. doi:10.1111/j.1365-2958.2007.05796.x. PMID 17590231.
- ↑ "A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)". Proceedings of the National Academy of Sciences of the United States of America 81 (7): 1966–1970. April 1984. doi:10.1073/pnas.81.7.1966. PMID 6201848. Bibcode: 1984PNAS...81.1966M.
- ↑ "A new Vibrio cholerae sRNA modulates colonization and affects release of outer membrane vesicles". Molecular Microbiology 70 (1): 100–111. October 2008. doi:10.1111/j.1365-2958.2008.06392.x. PMID 18681937.
- ↑ "Hfq is necessary for regulation by the untranslated RNA DsrA". Journal of Bacteriology 183 (6): 1997–2005. March 2001. doi:10.1128/JB.183.6.1997-2005.2001. PMID 11222598.
- ↑ "Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli". Genes & Development 17 (19): 2374–2383. October 2003. doi:10.1101/gad.1127103. PMID 12975324.
Further reading
- "The small RNA regulators of Escherichia coli: roles and mechanisms*". Annual Review of Microbiology 58: 303–328. 2004. doi:10.1146/annurev.micro.58.030603.123841. PMID 15487940. https://zenodo.org/record/1235035.
- "A genome-wide survey of sRNAs in the symbiotic nitrogen-fixing alpha-proteobacterium Sinorhizobium meliloti". BMC Genomics 11: 245. April 2010. doi:10.1186/1471-2164-11-245. PMID 20398411.
- "Deep sequencing of Salmonella RNA associated with heterologous Hfq proteins in vivo reveals small RNAs as a major target class and identifies RNA processing phenotypes". RNA Biology 6 (3): 266–275. July 2009. doi:10.4161/rna.6.3.8332. PMID 19333007.
External links
 |  |
