Biology:MSH6

From HandWiki
Revision as of 15:44, 13 February 2024 by Unex (talk | contribs) (link)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Short description: Protein-coding gene in the species Homo sapiens


A representation of the 3D structure of the protein myoglobin showing turquoise α-helices.
Generic protein structure example

MSH6 or mutS homolog 6 is a gene that codes for DNA mismatch repair protein Msh6 in the budding yeast Saccharomyces cerevisiae. It is the homologue of the human "G/T binding protein," (GTBP) also called p160 or hMSH6 (human MSH6). The MSH6 protein is a member of the Mutator S (MutS) family of proteins that are involved in DNA damage repair.

Defects in hMSH6 are associated with atypical hereditary nonpolyposis colorectal cancer not fulfilling the Amsterdam criteria for HNPCC. hMSH6 mutations have also been linked to endometrial cancer and the development of endometrial carcinomas.

Discovery

MSH6 was first identified in the budding yeast S. cerevisiae because of its homology to MSH2. The identification of the human GTBP gene and subsequent amino acid sequence availability showed that yeast MSH6 and human GTBP were more related to each other than any other MutS homolog, with a 26.6% amino acid identity.[1] Thus, GTBP took on the name human MSH6, or hMSH6.

Structure

In the human genome, hMSH6 is located on chromosome 2. It contains the Walker-A/B adenine nucleotide binding motif, which is the most highly conserved sequence found in all MutS homologs.[2] As with other MutS homologs, hMSH6 has an intrinsic ATPase activity. It functions exclusively when bound to hMSH2 as a heterodimer, although hMSH2 itself can function as a homomultimer or as a heterodimer with hMSH3.[3]

Function

Importance of mismatch repair

Mismatches commonly occur as a result of DNA replication errors, genetic recombination, or other chemical and physical factors.[4] Recognizing those mismatches and repairing them is extremely important for cells, because failure to do so results in microsatellite instability, an elevated spontaneous mutation rate (mutator phenotype), and susceptibility to HNPCC.[2][5] hMSH6 combines with hMSH2 to form the active protein complex, hMutS alpha, also called hMSH2-hMSH6.

Mismatch recognition

Mismatch recognition by this complex is regulated by the ADP to ATP transformation, which provides evidence that hMutS alpha complex functions as a molecular switch.[6] In normal DNA, adenine (A) bonds with thymine (T) and cytosine (C) bonds with guanine (G). Sometimes there will be a mismatch where T will bind with G, which is called a G/T mismatch. When a G/T mismatch is recognized, hMutS alpha complex binds and exchanges ADP for ATP.[5] The ADP-->ATP exchange causes a conformational change to convert hMutS alpha into a sliding clamp that can diffuse along the DNA backbone.[5] The ATP induces a release of the complex from the DNA and allows the hMutS alpha to dissociate along the DNA like a sliding clamp. This transformation helps trigger downstream events to repair the damaged DNA.[5]

Cancer

Although mutations in hMSH2 cause a strong general mutator phenotype, mutations in hMSH6 cause only a modest mutator phenotype.[1] At the gene level, the mutations were found to cause primarily single-base substitution mutations, which suggests that the role of hMSH6 is primarily for correcting single-base substitution mutations and to a lesser extent single base insertion/deletion mutations.[1]

Mutations in the hMSH6 gene cause the protein to be nonfunctional or only partially active, thus reducing its ability to repair mistakes in DNA. The loss of MSH6 function results in instability at mononucleotide repeats.[1] HNPCC is most commonly caused by mutations in hMSH2 and hMLH1, but mutations in hMSH6 are linked to an atypical form of HNPCC.[7] The penetrance of colorectal cancer seems to be lower in these mutations, meaning that a low proportion of hMSH6 mutation carriers present with the disease. Endometrial cancer, on the other hand, seems to be a more important clinical manifestation for female mutation carriers. The onset of endometrial cancer and also colon cancer in families with hMSH6 mutations is about 50 years. This is delayed compared to the age 44 onset of hMSH2-related tumors.[7]

Epigenetic control of MSH6 in cancer

Two microRNAs, miR21 and miR-155, target the DNA mismatch repair (MMR) genes hMSH6 and hMSH2, to cause reduced expression of their proteins.[8][9] If one or the other of these two microRNAs is over-expressed, hMSH2 and hMSH6 proteins are under-expressed, resulting in reduced DNA mismatch repair and increased microsatellite instability.

One of these microRNAs, miR21, is regulated by the epigenetic methylation state of the CpG islands in one or the other of its two promoter regions.[10] Hypomethylation of its promoter region is associated with increased expression of an miRNA.[11] High expression of a microRNA causes repression of its target genes (see microRNA silencing of genes). In 66% to 90% of colon cancers, miR-21 was over-expressed,[8] and generally the measured level of hMSH2 was decreased (and hMSH6 is unstable without hMSH2[9]).

The other microRNA, miR-155, is regulated both by epigenetic methylation of the CpG islands in its promoter region[12] and by epigenetic acetylation of histones H2A and H3 at the miR-155 promoter (where acetylation increases transcription).[13] Measured by two different methods, miR-155 was over-expressed in sporadic colorectal cancers by either 22% or 50%.[9] When miR-155 was elevated, hMSH2 was under-expressed in 44% to 67% of the same tissues (and hMSH6 is likely under-expressed as well, and also unstable in the absence of hMSH2).[9]

Interactions

MSH6 has been shown to interact with MSH2,[14][15][16][17][18] PCNA[19][20][21] and BRCA1.[14][22]

See also

References

  1. ↑ 1.0 1.1 1.2 1.3 "Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair". Genes Dev. 10 (4): 407–20. 1996. doi:10.1101/gad.10.4.407. PMID 8600025. 
  2. ↑ 2.0 2.1 "Identification of mismatch repair genes and their role in the development of cancer". Current Opinion in Genetics & Development 5 (3): 382–95. 1995. doi:10.1016/0959-437X(95)80055-7. PMID 7549435. 
  3. ↑ "hMSH2 forms specific mispair-binding complexes with hMSH3 and hMSH6". PNAS 93 (24): 13629–34. 1996. doi:10.1073/pnas.93.24.13629. PMID 8942985. Bibcode: 1996PNAS...9313629A. 
  4. ↑ Friedberg EC, Walker GC, Siede W. (1995). DNA repair and mutagenesis. American Society for Microbiology, Washington DC.
  5. ↑ 5.0 5.1 5.2 5.3 "hMSH2-hMSH6 forms a hydrolysis-independent sliding clamp on mismatched DNA". Molecular Cell 3 (2): 255–61. 1999. doi:10.1016/S1097-2765(00)80316-0. PMID 10078208. 
  6. ↑ "The human mismatch recognition complex hMSH2-hMSH6 functions as a novel molecular switch". Cell 91 (7): 995–1005. 1997. doi:10.1016/S0092-8674(00)80490-0. PMID 9428522. 
  7. ↑ 7.0 7.1 "Atypical HNPCC owing to MSH6 germline mutations: analysis of a large Dutch pedigree". J. Med. Genet. 38 (5): 318–22. 2001. doi:10.1136/jmg.38.5.318. PMID 11333868. 
  8. ↑ 8.0 8.1 "MicroRNA-21 induces resistance to 5-fluorouracil by down-regulating human DNA MutS homolog 2 (hMSH2)". Proc. Natl. Acad. Sci. U.S.A. 107 (49): 21098–103. 2010. doi:10.1073/pnas.1015541107. PMID 21078976. Bibcode: 2010PNAS..10721098V. 
  9. ↑ 9.0 9.1 9.2 9.3 "Modulation of mismatch repair and genomic stability by miR-155". Proc. Natl. Acad. Sci. U.S.A. 107 (15): 6982–7. 2010. doi:10.1073/pnas.1002472107. PMID 20351277. Bibcode: 2010PNAS..107.6982V. 
  10. ↑ "Genome-wide epigenetic regulation of miRNAs in cancer". Cancer Res. 73 (2): 473–7. 2013. doi:10.1158/0008-5472.CAN-12-3731. PMID 23316035. 
  11. ↑ "Individual and combined effects of DNA methylation and copy number alterations on miRNA expression in breast tumors". Genome Biol. 14 (11): R126. 2013. doi:10.1186/gb-2013-14-11-r126. PMID 24257477. 
  12. ↑ "Insights into epigenetic regulation of microRNA-155 expression in multiple myeloma". Biochim. Biophys. Acta 1849 (3): 353–66. 2015. doi:10.1016/j.bbagrm.2014.12.002. PMID 25497370. 
  13. ↑ "Tumor suppressor BRCA1 epigenetically controls oncogenic microRNA-155". Nat. Med. 17 (10): 1275–82. 2011. doi:10.1038/nm.2459. PMID 21946536. 
  14. ↑ 14.0 14.1 "BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures". Genes & Development 14 (8): 927–39. Apr 2000. doi:10.1101/gad.14.8.927. PMID 10783165. 
  15. ↑ "MSH2 and ATR form a signaling module and regulate two branches of the damage response to DNA methylation". Proceedings of the National Academy of Sciences of the United States of America 100 (26): 15387–92. Dec 2003. doi:10.1073/pnas.2536810100. PMID 14657349. Bibcode: 2003PNAS..10015387W. 
  16. ↑ "Interactions of human hMSH2 with hMSH3 and hMSH2 with hMSH6: examination of mutations found in hereditary nonpolyposis colorectal cancer". Molecular and Cellular Biology 18 (11): 6616–23. Nov 1998. doi:10.1128/mcb.18.11.6616. PMID 9774676. 
  17. ↑ "hMSH5: a human MutS homologue that forms a novel heterodimer with hMSH4 and is expressed during spermatogenesis". Cancer Research 59 (4): 816–22. Feb 1999. PMID 10029069. 
  18. ↑ "hMSH2 forms specific mispair-binding complexes with hMSH3 and hMSH6". Proceedings of the National Academy of Sciences of the United States of America 93 (24): 13629–34. Nov 1996. doi:10.1073/pnas.93.24.13629. PMID 8942985. Bibcode: 1996PNAS...9313629A. 
  19. ↑ "hMSH3 and hMSH6 interact with PCNA and colocalize with it to replication foci". Genes & Development 15 (6): 724–36. Mar 2001. doi:10.1101/gad.191201. PMID 11274057. 
  20. ↑ "Functional interaction of proliferating cell nuclear antigen with MSH2-MSH6 and MSH2-MSH3 complexes". The Journal of Biological Chemistry 275 (47): 36498–501. Nov 2000. doi:10.1074/jbc.C000513200. PMID 11005803. 
  21. ↑ "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry 277 (43): 40362–7. Oct 2002. doi:10.1074/jbc.M206194200. PMID 12171929. 
  22. ↑ "Adenosine nucleotide modulates the physical interaction between hMSH2 and BRCA1". Oncogene 20 (34): 4640–9. Aug 2001. doi:10.1038/sj.onc.1204625. PMID 11498787. 

Further reading

External links



Retrieved from "https://handwiki.org/wiki/index.php?title=Biology:MSH6&oldid=3653546"