Biology:PC3
PC3 (PC-3) is a human prostate cancer cell line used in prostate cancer research and drug development. PC3 cells are useful in investigating biochemical changes in advanced prostate cancer cells and in assessing their response to chemotherapeutic agents. PC3 cells are also used to study viral infection in mammalian cells that exhibit an immune response[1]
Description
The PC3 cell line was established in 1979 from bone metastasis of grade IV of prostate cancer in a 62-year-old Caucasian male.[2] These cells do not respond to androgens, glucocorticoids or fibroblast growth factors,[3] but results suggest that the cells are influenced by epidermal growth factors.[4] PC3 cells can be used to create subcutaneous tumor xenografts in mice to investigate the tumor environment and therapeutic drug functionality.[citation needed]
PC3 cells have high metastatic potential compared to DU145 cells, which have a moderate metastatic potential, and to LNCaP cells, which have low metastatic potential.[5] Comparisons of the protein expression of PC3, LNCaP, and other cells have shown that PC3 is characteristic of small cell neoendocrine carcinoma.[3]
PC3 cells have low testosterone-5-alpha reductase and acidic phosphatase activity, and do not express PSA (prostate-specific antigen). Furthermore, karyotypic analysis has shown that PC3 are near-triploid, having 62 chromosomes. Q-band analysis showed no Y chromosome. From a morphological point of view, electron microscopy revealed that PC3 cells show characteristics of a poorly-differentiated adenocarcinoma. They have features common to neoplastic cells of epithelial origins, such as numerous microvilli, junctional complexes, abnormal nuclei and nucleoli, abnormal mitochondria, annulate lamellae, and lipoidal bodies.[citation needed]
See also
References
- ↑ "Robust kinetics of an RNA virus: Transcription rates are set by genome levels". Biotechnology and Bioengineering 112 (8): 1655–62. August 2015. doi:10.1002/bit.25578. PMID 25726926.
- ↑ "Establishment and characterization of a human prostatic carcinoma cell line (PC-3)". Investigative Urology 17 (1): 16–23. July 1979. PMID 447482. https://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=447482&dopt=AbstractPlus.
- ↑ 3.0 3.1 "PC3 is a cell line characteristic of prostatic small cell carcinoma". The Prostate 71 (15): 1668–79. November 2011. doi:10.1002/pros.21383. PMID 21432867.
- ↑ "Estimating cell diffusivity and cell proliferation rate by interpreting IncuCyte ZOOM™ assay data using the Fisher-Kolmogorov model". BMC Systems Biology 9 (38): 38. July 2015. doi:10.1186/s12918-015-0182-y. PMID 26188761.
- ↑ "RNA interference-directed knockdown of urokinase plasminogen activator and urokinase plasminogen activator receptor inhibits prostate cancer cell invasion, survival, and tumorigenicity in vivo". The Journal of Biological Chemistry 280 (43): 36529–40. October 2005. doi:10.1074/jbc.M503111200. PMID 16127174.
External links
- Information on PC3 in the ATCC catalog
- Cellosaurus entry for PC3
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