Biology:3-methyl-2-oxobutanoate dehydrogenase
| 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 2-oxoisovalerate dehydrogenase heterotetramer, Human | |||||||||
| Identifiers | |||||||||
| EC number | 1.2.4.4 | ||||||||
| CAS number | 9082-72-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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In enzymology, a 3-methyl-2-oxobutanoate dehydrogenase (EC 1.2.4.4) is an enzyme that catalyzes the chemical reaction
- 3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine [math]\displaystyle{ \rightleftharpoons }[/math] [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2
The 3 substrates of this enzyme are 3-methyl-2-oxobutanoate, dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, and lipoyllysine, whereas its 3 products are dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, S-(2-methylpropanoyl)dihydrolipoyllysine, and CO2.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with a disulfide as acceptor.
This enzyme participates in valine, leucine and isoleucine degradation. It employs one cofactor, thiamin diphosphate. It is the E1 subunit of a catalytic complex.
Structural studies
As of late 2007, twenty-nine structures have been solved for this class of enzymes, with PDB accession codes 1DTW, 1OLS, 1OLU, 1OLX, 1U5B, 1UM9, 1UMB, 1UMC, 1UMD, 1V11, 1V16, 1V1M, 1V1R, 1WCI, 1X7W, 1X7X, 1X7Y, 1X7Z, 1X80, 2BEU, 2BEV, 2BEW, 2BFB, 2BFC, 2BFD, 2BFE, 2BFF, 2BP7, and 2J9F.
References
- "Branched chain alpha-keto acid metabolism. II. Evidence for the common identity of alpha-ketoisocaproic acid and alpha-keto-beta-methyl-valeric acid dehydrogenases". J. Biol. Chem. 243 (12): 3526–31. 1968. doi:10.1016/S0021-9258(18)93339-9. PMID 5656388.
- "Branched chain alpha-keto acid metabolism. I. Isolation, purification, and partial characterization of bovine liver alpha-ketoisocaproic:alpha-keto-beta-methylvaleric acid dehydrogenase". J. Biol. Chem. 243 (6): 1198–203. 1968. doi:10.1016/S0021-9258(19)56972-1. PMID 5689906.
- "Purification and characterization of branched chain alpha-ketoacid dehydrogenase from bovine liver mitochondria". J. Biol. Chem. 254 (12): 5522–6. 1979. doi:10.1016/S0021-9258(18)50626-8. PMID 447664.
- "Purification and characterization of branched chain alpha-keto acid dehydrogenase complex of bovine kidney". Proc. Natl. Acad. Sci. U.S.A. 75 (10): 4881–5. 1978. doi:10.1073/pnas.75.10.4881. PMID 283398. Bibcode: 1978PNAS...75.4881P.
- Perham RN (2000). "Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions". Annu. Rev. Biochem. 69 (1): 961–1004. doi:10.1146/annurev.biochem.69.1.961. PMID 10966480.
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