Biology:CLSPN

Generic protein structure example

Claspin is a protein that in humans is encoded by the CLSPN gene.

Function

Xenopus claspin is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in the presence of DNA templates in Xenopus egg extracts. The human gene appears to be the homolog Xenopus claspin and its function has not been determined.

Interactions

CLSPN has been shown to interact with:

• BRCA1,
• CDC45,
• CHEK1,
• POLE,
• RAD17, and
• USP7.

External links

• Human CLSPN genome location and CLSPN gene details page in the UCSC Genome Browser.

Further reading

• "Claspin, a regulator of Chk1 in DNA replication stress pathway.". DNA Repair (Amst.) 3 (8–9): 1033–7. 2005. doi:10.1016/j.dnarep.2004.03.001. PMID 15279790. 
• "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. 2003. doi:10.1073/pnas.242603899. PMID 12477932. 
• "Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation.". Proc. Natl. Acad. Sci. U.S.A. 101 (17): 6484–9. 2004. doi:10.1073/pnas.0401847101. PMID 15096610. Bibcode: 2004PNAS..101.6484L. 
• "ATR, Claspin and the Rad9-Rad1-Hus1 complex regulate Chk1 and Cdc25A in the absence of DNA damage.". Cell Cycle 3 (7): 941–5. 2006. doi:10.4161/cc.3.7.972. PMID 15190204. 
• "Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures.". J. Biol. Chem. 279 (38): 39289–95. 2004. doi:10.1074/jbc.M405793200. PMID 15226314. 
• "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. 2004. doi:10.1101/gr.2596504. PMID 15489334. 
• "DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system.". Biochem. J. 388 (Pt 2): 705–12. 2005. doi:10.1042/BJ20041966. PMID 15707391. 
• "Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway.". J. Biol. Chem. 280 (42): 35337–45. 2005. doi:10.1074/jbc.M506460200. PMID 16123041. 
• "Chk1 is required to maintain claspin stability.". Oncogene 25 (30): 4165–71. 2006. doi:10.1038/sj.onc.1209447. PMID 16501606. 
• "The DNA sequence and biological annotation of human chromosome 1.". Nature 441 (7091): 315–21. 2006. doi:10.1038/nature04727. PMID 16710414. Bibcode: 2006Natur.441..315G. 
• "Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation.". FEBS Lett. 580 (17): 4176–81. 2006. doi:10.1016/j.febslet.2006.06.071. PMID 16828751. 
• "Claspin operates downstream of TopBP1 to direct ATR signaling towards Chk1 activation.". Mol. Cell. Biol. 26 (16): 6056–64. 2006. doi:10.1128/MCB.00492-06. PMID 16880517. 
• "Destruction of Claspin by SCFbetaTrCP restrains Chk1 activation and facilitates recovery from genotoxic stress.". Mol. Cell 23 (3): 307–18. 2006. doi:10.1016/j.molcel.2006.06.016. PMID 16885021. 
• "SCFbetaTrCP-mediated degradation of Claspin regulates recovery from the DNA replication checkpoint response.". Mol. Cell 23 (3): 319–29. 2006. doi:10.1016/j.molcel.2006.06.013. PMID 16885022. 
• "Polo-like kinase-1 controls proteasome-dependent degradation of Claspin during checkpoint recovery.". Curr. Biol. 16 (19): 1950–5. 2006. doi:10.1016/j.cub.2006.08.026. PMID 16934469. 
• "Chk1- and claspin-dependent but ATR/ATM- and Rad17-independent DNA replication checkpoint response in HeLa cells.". Cancer Res. 66 (17): 8672–9. 2007. doi:10.1158/0008-5472.CAN-05-4443. PMID 16951182. 
• "Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function.". J. Biol. Chem. 281 (44): 33276–82. 2006. doi:10.1074/jbc.M604373200. PMID 16963448. 
• "Characterization of functional domains in human Claspin.". Cell Cycle 10 (10): 1599–606. 2011. doi:10.4161/cc.10.10.15562. PMID 21478680.