Biology:Expression cassette
An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell.[1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette can easily be altered to make different proteins.[2]
An expression cassette is composed of one or more genes and the sequences controlling their expression.[3] An expression cassette comprises three components: a promoter sequence, an open reading frame, and a 3' untranslated region that, in eukaryotes, usually contains a polyadenylation site.[4]
Different expression cassettes can be transfected into different organisms including bacteria, yeast, plants, and mammalian cells as long as the correct regulatory sequences are used.[5][6]
See also
References
- ↑ Papadakis, E.D. (2004). "Promoters and Control Elements: Designing Expression Cassettes for Gene Therapy". Current Gene Therapy 4 (4): 89–113. doi:10.2174/1566523044578077. PMID 15032617.
- ↑ Bertrand, Edouard (1997). "The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization". RNA 3 (1): 75–88. PMID 8990401.
- ↑ Vickers, Claudia E (December 2013). "Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae". Microbial Cell Factories 12 (96): 96. doi:10.1186/1475-2859-12-96. PMID 24161108.
- ↑ Gray, Steven James (January 2015). "Viral Expression Cassette Elements to Enhance Transgene Target Specificity and Expression in Gene Therapy". Discov Med 19 (102): 49–57. ISSN 1539-6509. PMID 25636961.
- ↑ Azhakanandam, K. (2015). "Plant Trait Gene Expression Cassette Design". Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants. Springer. pp. 41–52. doi:10.1007/978-1-4939-2202-4_2. ISBN 978-1-4939-2201-7.
- ↑ Chow, Yu-Hua (December 1997). "Development of an epithelium-specific expression cassette with human DNA regulatory elements for transgene expression in lung airways". Proceedings of the National Academy of Sciences 94 (26): 14695–14700. doi:10.1073/pnas.94.26.14695. PMID 9405675. PMC 25096. Bibcode: 1997PNAS...9414695C. http://www.pnas.org/content/94/26/14695.full.pdf.
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