Biology:Glutathione-ascorbate cycle
The ascorbate-glutathione cycle, sometimes Foyer-Halliwell-Asada pathway, is a metabolic pathway that detoxifies hydrogen peroxide (H2O2), a reactive oxygen species that is produced as a waste product in metabolism. The cycle involves the antioxidant metabolites: ascorbate, glutathione and NADPH and the enzymes linking these metabolites.[1]
In the first step of this pathway, H2O2 is reduced to water by ascorbate peroxidase (APX) using ascorbate (ASC) as the electron donor. The oxidized ascorbate (monodehydroascorbate, MDA) is regenerated by monodehydroascorbate reductase (MDAR).[2] However, monodehydroascorbate is a radical and if not rapidly reduced it disproportionates into ascorbate and dehydroascorbate (DHA). Dehydroascorbate is reduced to ascorbate by dehydroascorbate reductase (DHAR) at the expense of GSH, yielding oxidized glutathione (GSSG). Finally GSSG is reduced by glutathione reductase (GR) using NADPH as the electron donor. Thus ascorbate and glutathione are not consumed; the net electron flow is from NADPH to H2O2. The reduction of dehydroascorbate may be non-enzymatic or catalysed by proteins with dehydroascorbate reductase activity, such as glutathione S-transferase omega 1 or glutaredoxins.[3][4]
In plants, the glutathione-ascorbate cycle operates in the cytosol, mitochondria, plastids and peroxisomes.[5][6] Since glutathione, ascorbate and NADPH are present in high concentrations in plant cells it is assumed that the glutathione-ascorbate cycle plays a key role for H2O2 detoxification. Nevertheless, other enzymes (peroxidases) including peroxiredoxins and glutathione peroxidases, which use thioredoxins or glutaredoxins as reducing substrates, also contribute to H2O2 removal in plants.[7]
See also
- Antioxidant
- Oxidative stress
- Peroxidases
References
- ↑ "ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control". Annu Rev Plant Physiol Plant Mol Biol 49: 249–279. Jun 1998. doi:10.1146/annurev.arplant.49.1.249. PMID 15012235.
- ↑ "Dehydroascorbate reduction". J. Bioenerg. Biomembr. 26 (4): 369–77. August 1994. doi:10.1007/BF00762777. PMID 7844111.
- ↑ "Characterization of the omega class of glutathione transferases". Meth. Enzymol.. Methods in Enzymology 401: 78–99. 2005. doi:10.1016/S0076-6879(05)01005-0. ISBN 9780121828066. PMID 16399380.
- ↑ "Exploring the active site of plant glutaredoxin by site-directed mutagenesis". FEBS Lett 511 (1–3): 145–9. 2002. doi:10.1016/S0014-5793(01)03302-6. PMID 11821065.
- ↑ Meyer A (Sep 2009). "The integration of glutathione homeostasis and redox signaling". J Plant Physiol 165 (13): 1390–403. doi:10.1016/j.jplph.2007.10.015. PMID 18171593.
- ↑ "Role of the Ascorbate-Glutathione Cycle of Mitochondria and Peroxisomes in the Senescence of Pea Leaves". Plant Physiol 118 (4): 1327–35. Dec 1998. doi:10.1104/pp.118.4.1327. PMID 9847106.
- ↑ "The role of glutathione in photosynthetic organisms: emerging functions for glutaredoxins and glutathionylation". Annu Rev Plant Biol 59: 143–66. 2008. doi:10.1146/annurev.arplant.59.032607.092811. PMID 18444899. https://hal.inrae.fr/hal-02660326/file/2008%20Rouhier%20Jacquot%20ARPB.pdf.
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