Biology:Glutathione peroxidase
| Glutathione peroxidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Crystallographic structure of bovine glutathione peroxidase 1.[1] | |||||||||
| Identifiers | |||||||||
| EC number | 1.11.1.9 | ||||||||
| CAS number | 9013-66-5 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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| Glutathione peroxidase | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||||
| Symbol | GSHPx | ||||||||||
| Pfam | PF00255 | ||||||||||
| InterPro | IPR000889 | ||||||||||
| PROSITE | PDOC00396 | ||||||||||
| SCOP2 | 1gp1 / SCOPe / SUPFAM | ||||||||||
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Glutathione peroxidase (GPx) (EC 1.11.1.9) is the general name of an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage.[2] The biochemical function of glutathione peroxidase is to reduce lipid hydroperoxides to their corresponding alcohols and to reduce free hydrogen peroxide to water.[3]
Isozymes
Several isozymes are encoded by different genes, which vary in cellular location and substrate specificity. Glutathione peroxidase 1 (GPx1) is the most abundant version, found in the cytoplasm of nearly all mammalian tissues, whose preferred substrate is hydrogen peroxide. Glutathione peroxidase 4 (GPx4) has a high preference for lipid hydroperoxides; it is expressed in nearly every mammalian cell, though at much lower levels. Glutathione peroxidase 2 is an intestinal and extracellular enzyme, while glutathione peroxidase 3 is extracellular, especially abundant in plasma.[4] So far, eight different isoforms of glutathione peroxidase (GPx1-8) have been identified in humans.
| Gene | Locus | Enzyme |
|---|---|---|
| GPX1 | Chr. 3 p21.3 | glutathione peroxidase 1 |
| GPX2 | Chr. 14 q24.1 | glutathione peroxidase 2 (gastrointestinal) |
| GPX3 | Chr. 5 q23 | glutathione peroxidase 3 (plasma) |
| GPX4 | Chr. 19 p13.3 | glutathione peroxidase 4 (phospholipid hydroperoxidase) |
| GPX5 | Chr. 6 p21.32 | glutathione peroxidase 5 (epididymal androgen-related protein) |
| GPX6 | Chr. 6 p21 | glutathione peroxidase 6 (olfactory) |
| GPX7 | Chr. 1 p32 | glutathione peroxidase 7 |
| GPX8 | Chr. 5 q11.2 | glutathione peroxidase 8 (putative) |
Reaction
The main reaction that glutathione peroxidase catalyzes is:
- 2GSH + H2O2 → GS–SG + 2H2O
where GSH represents reduced monomeric glutathione, and GS–SG represents glutathione disulfide. The mechanism involves oxidation of the selenol of a selenocysteine residue by hydrogen peroxide. This process gives the derivative with a selenenic acid (RSeOH) group. The selenenic acid is then converted back to the selenol by a two step process that begins with reaction with GSH to form the GS-SeR and water. A second GSH molecule reduces the GS-SeR intermediate back to the selenol, releasing GS-SG as the by-product. A simplified representation is shown below:[5]
- RSeH + H2O2 → RSeOH + H2O
- RSeOH + GSH → GS-SeR + H2O
- GS-SeR + GSH → GS-SG + RSeH
Glutathione reductase then reduces the oxidized glutathione to complete the cycle:
- GS–SG + NADPH + H+ → 2 GSH + NADP+.
Structure
Mammalian GPx1, GPx2, GPx3, and GPx4 have been shown to be selenium-containing enzymes, whereas GPx6 is a selenoprotein in humans with cysteine-containing homologues in rodents. GPx1, GPx2, and GPx3 are homotetrameric proteins, whereas GPx4 has a monomeric structure. As the integrity of the cellular and subcellular membranes depends heavily on glutathione peroxidase, its antioxidative protective system itself depends heavily on the presence of selenium.
Animal models
Mice genetically engineered to lack glutathione peroxidase 1 (Gpx1−/− mice) are grossly phenotypically normal and have normal lifespans, indicating this enzyme is not critical for life. However, Gpx1−/− mice develop cataracts at an early age and exhibit defects in muscle satellite cell proliferation.[4] Gpx1 −/− mice showed up to 16 dB higher auditory brainstem response (ABR) thresholds than control mice. After 110 dB noise exposure for one hour, Gpx1 −/− mice had up to 15 dB greater noise-induced hearing loss compared with control mice.[6]"
Mice with knockouts for GPX3 (GPX3−/−) or GPX2 (GPX2−/−) also develop normally [7][8]
However, glutathione peroxidase 4 knockout mice die during early embryonic development.[4] Some evidence, though, indicates reduced levels of glutathione peroxidase 4 can increase life expectancy in mice.[9]
The bovine erythrocyte enzyme has a molecular weight of 84 kDa.
Discovery
Glutathione peroxidase was discovered in 1957 by Gordon C. Mills.[10]
Methods for determining glutathione peroxidase activity
Activity of glutathione peroxidase is measured spectrophotometrically using several methods. A direct assay by linking the peroxidase reaction with glutathione reductase with measurement of the conversion of NADPH to NADP is widely used.[11] The other approach is measuring residual GSH in the reaction with Ellman's reagent. Based on this, several procedures for measuring glutathione peroxidase activity were developed using various hydroperoxides as substrates for reduction, e.g. cumene hydroperoxide,[12] tert-butyl hydroperoxide [13] and hydrogen peroxide.[14]
The other methods include the use of CUPRAC reagent with spectrophotometric detection of the reaction product[15] or o-phtalaldehyde as a fluorescent reagent.[16]
Clinical significance
It has been shown that low levels of glutathione peroxidase as measured in the serum may be a contributing factor to vitiligo.[17] Lower plasma glutathione peroxide levels were also observed in patients with type 2 diabetes with macroalbuminuria and this was correlated to the stage of diabetic nephropathy.[citation needed] In one study, the activity of glutathione peroxidase along with other antioxidant enzymes such as superoxide dismutase and catalase was not associated with coronary heart disease risk in women.[18] Glutathione peroxidase activity was found to be much lower in patients with relapsing-remitting multiple sclerosis.[19] One study has suggested that glutathione peroxidase and superoxide dismutase polymorphisms play a role in the development of celiac disease.[20]
The activity of this enzyme has been reported to be decreased in case of copper deficiency in the liver and plasma.[21]
See also
References
- ↑ PDB: 1GP1; "The refined structure of the selenoenzyme glutathione peroxidase at 0.2-nm resolution". European Journal of Biochemistry 133 (1): 51–69. June 1983. doi:10.1111/j.1432-1033.1983.tb07429.x. PMID 6852035.
- ↑ "Cadmium-induced oxidative stress in Saccharomyces cerevisiae". Indian Journal of Biochemistry & Biophysics 47 (6): 383–7. December 2010. PMID 21355423. http://nopr.niscair.res.in/handle/123456789/10863.
- ↑ "Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress". Antonie van Leeuwenhoek 99 (4): 761–71. May 2011. doi:10.1007/s10482-011-9550-9. PMID 21229313.
- ↑ 4.0 4.1 4.2 "Trends in oxidative aging theories". Free Radical Biology & Medicine 43 (4): 477–503. August 2007. doi:10.1016/j.freeradbiomed.2007.03.034. PMID 17640558.
- ↑ "Functional mimics of glutathione peroxidase: bioinspired synthetic antioxidants". Accounts of Chemical Research 43 (11): 1408–19. November 2010. doi:10.1021/ar100059g. PMID 20690615.
- ↑ "Targeted mutation of the gene for cellular glutathione peroxidase (Gpx1) increases noise-induced hearing loss in mice". Journal of the Association for Research in Otolaryngology 1 (3): 243–54. November 2000. doi:10.1007/s101620010043. PMID 11545230.
- ↑ "Mice with combined disruption of Gpx1 and Gpx2 genes have colitis". American Journal of Physiology. Gastrointestinal and Liver Physiology 281 (3): G848-55. September 2001. doi:10.1152/ajpgi.2001.281.3.G848. PMID 11518697.
- ↑ "Extracellular glutathione peroxidase (Gpx3) binds specifically to basement membranes of mouse renal cortex tubule cells". American Journal of Physiology. Renal Physiology 298 (5): F1244-53. May 2010. doi:10.1152/ajprenal.00662.2009. PMID 20015939.
- ↑ "Reduction in glutathione peroxidase 4 increases life span through increased sensitivity to apoptosis". The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences 62 (9): 932–42. September 2007. doi:10.1093/gerona/62.9.932. PMID 17895430.
- ↑ "Hemoglobin catabolism. I. Glutathione peroxidase, an erythrocyte enzyme which protects hemoglobin from oxidative breakdown". The Journal of Biological Chemistry 229 (1): 189–97. November 1957. doi:10.1016/S0021-9258(18)70608-X. PMID 13491573.
- ↑ "Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase". The Journal of Laboratory and Clinical Medicine 70 (1): 158–69. July 1967. PMID 6066618.
- ↑ "A semiautomated system for measurement of glutathione in the assay of glutathione peroxidase". Analytical Biochemistry 89 (2): 430–6. September 1978. doi:10.1016/0003-2697(78)90372-X. PMID 727443.
- ↑ "[A simple and specific method for determining glutathione peroxidase activity in erythrocytes]". Laboratornoe Delo 12 (12): 724–7. 1986. PMID 2434712.
- ↑ "Correction to: A Method of Measuring Glutathione Peroxidase Activity in Murine Brain: Application in Pharmacological Experiment". Bulletin of Experimental Biology and Medicine 165 (4): 589–592. August 2018. doi:10.1007/s10517-018-4219-2. PMID 30121905.
- ↑ Ahmed, A. Y.; Aowda, S. A.; Hadwan, M. H. (2021). "A validated method to assess glutathione peroxidase enzyme activity" (in en). Chemical Papers 75 (12): 6625–6637. doi:10.1007/s11696-021-01826-1. ISSN 2585-7290. https://doi.org/10.1007/s11696-021-01826-1.
- ↑ Ramos Martinez, J. I.; Launay, J.-M.; Dreux, C. (1979). "A sensitive fluorimetric microassay for the determination of glutathione peroxidase activity. Application to human blood platelets" (in en). Analytical Biochemistry 98 (1): 154–159. doi:10.1016/0003-2697(79)90720-6. ISSN 0003-2697. https://dx.doi.org/10.1016/0003-2697%2879%2990720-6.
- ↑ "Low glutathione peroxidase activity levels in patients with vitiligo". Journal of Cutaneous Medicine and Surgery 19 (2): 144–8. Mar 2015. doi:10.2310/7750.2014.14076. PMID 25775636.
- ↑ "Erythrocyte superoxide dismutase, glutathione peroxidase, and catalase activities and risk of coronary heart disease in generally healthy women: a prospective study". American Journal of Epidemiology 180 (9): 901–8. November 2014. doi:10.1093/aje/kwu195. PMID 25156995.
- ↑ "Dietary habits and selenium, glutathione peroxidase and total antioxidant status in the serum of patients with relapsing-remitting multiple sclerosis". Nutrition Journal 13: 62. June 2014. doi:10.1186/1475-2891-13-62. PMID 24943732.
- ↑ "Evaluation of glutathione peroxidase and superoxide dismutase enzyme polymorphisms in celiac disease patients". Genetics and Molecular Research 13 (1): 1030–7. February 2014. doi:10.4238/2014.February.20.4. PMID 24634124.
- ↑ Hordyjewska, Anna; Popiołek, Łukasz; Kocot, Joanna (2014). "The many 'faces' of copper in medicine and treatment". Biometals 27 (4): 611–621. doi:10.1007/s10534-014-9736-5. PMID 24748564.
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