Biology:HUS1
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Checkpoint protein HUS1 is a protein that in humans is encoded by the HUS1 gene.[1][2]
Function
The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event.[3]
Interactions
HUS1 has been shown to interact with:
References
- ↑ "cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster". Genomics 54 (3): 424–36. Dec 1998. doi:10.1006/geno.1998.5587. PMID 9878245.
- ↑ "Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage". The EMBO Journal 17 (7): 2055–66. Apr 1998. doi:10.1093/emboj/17.7.2055. PMID 9524127.
- ↑ "Entrez Gene: HUS1 HUS1 checkpoint homolog (S. pombe)". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=3364.
- ↑ "HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins". The Journal of Biological Chemistry 275 (36): 27909–16. Sep 2000. doi:10.1074/jbc.M000168200. PMID 10846170.
- ↑ "PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition". Oncogene 19 (46): 5291–7. Nov 2000. doi:10.1038/sj.onc.1203901. PMID 11077446.
- ↑ 6.0 6.1 "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex". The Journal of Biological Chemistry 274 (2): 567–70. Jan 1999. doi:10.1074/jbc.274.2.567. PMID 9872989.
- ↑ "Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1". Genomics 79 (4): 487–92. Apr 2002. doi:10.1006/geno.2002.6737. PMID 11944979.
- ↑ "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro". Proceedings of the National Academy of Sciences of the United States of America 100 (4): 1633–8. Feb 2003. doi:10.1073/pnas.0437927100. PMID 12578958.
- ↑ "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9". The Journal of Biological Chemistry 275 (38): 29767–71. Sep 2000. doi:10.1074/jbc.M005782200. PMID 10884395.
- ↑ "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene". Genomics 82 (6): 644–51. Dec 2003. doi:10.1016/s0888-7543(03)00200-3. PMID 14611806.
- ↑ "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy". The Journal of Biological Chemistry 277 (18): 15233–6. May 2002. doi:10.1074/jbc.C200129200. PMID 11907025.
- ↑ "A role of the C-terminal region of human Rad9 (hRad9) in nuclear transport of the hRad9 checkpoint complex". The Journal of Biological Chemistry 277 (28): 25722–7. Jul 2002. doi:10.1074/jbc.M203079200. PMID 11994305.
Further reading
- "Cloning of ARE-containing genes by AU-motif-directed display". Genomics 54 (2): 278–86. Dec 1998. doi:10.1006/geno.1998.5548. PMID 9828130.
- "Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex". The Journal of Biological Chemistry 274 (2): 567–70. Jan 1999. doi:10.1074/jbc.274.2.567. PMID 9872989.
- "The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1". Molecular Biology of the Cell 10 (6): 1985–95. Jun 1999. doi:10.1091/mbc.10.6.1985. PMID 10359610.
- "Physical interactions among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression". Genomics 65 (1): 24–33. Apr 2000. doi:10.1006/geno.2000.6142. PMID 10777662.
- "HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins". The Journal of Biological Chemistry 275 (36): 27909–16. Sep 2000. doi:10.1074/jbc.M000168200. PMID 10846170.
- "Retention of the human Rad9 checkpoint complex in extraction-resistant nuclear complexes after DNA damage". The Journal of Biological Chemistry 275 (34): 26343–8. Aug 2000. doi:10.1074/jbc.M001244200. PMID 10852904.
- "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9". The Journal of Biological Chemistry 275 (38): 29767–71. Sep 2000. doi:10.1074/jbc.M005782200. PMID 10884395.
- "PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition". Oncogene 19 (46): 5291–7. Nov 2000. doi:10.1038/sj.onc.1203901. PMID 11077446.
- "The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9". Biochemical and Biophysical Research Communications 287 (4): 932–40. Oct 2001. doi:10.1006/bbrc.2001.5685. PMID 11573955. https://kanazawa-u.repo.nii.ac.jp/?action=repository_uri&item_id=27371.
- "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy". The Journal of Biological Chemistry 277 (18): 15233–6. May 2002. doi:10.1074/jbc.C200129200. PMID 11907025.
- "Downregulation of Hus1 by antisense oligonucleotides enhances the sensitivity of human lung carcinoma cells to cisplatin". Cancer 94 (6): 1808–14. Mar 2002. doi:10.1002/cncr.10383. PMID 11920544.
- "Identification and characterization of a paralog of human cell cycle checkpoint gene HUS1". Genomics 79 (4): 487–92. Apr 2002. doi:10.1006/geno.2002.6737. PMID 11944979.
- "Genotoxin-induced Rad9-Hus1-Rad1 (9-1-1) chromatin association is an early checkpoint signaling event". The Journal of Biological Chemistry 277 (46): 43809–12. Nov 2002. doi:10.1074/jbc.M207272200. PMID 12228248.
- "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro". Proceedings of the National Academy of Sciences of the United States of America 100 (4): 1633–8. Feb 2003. doi:10.1073/pnas.0437927100. PMID 12578958.
- "Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue". Cancer Research 63 (17): 5291–8. Sep 2003. PMID 14500360.
- "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene". Genomics 82 (6): 644–51. Dec 2003. doi:10.1016/S0888-7543(03)00200-3. PMID 14611806.
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