Biology:HuLEC-5a
Human Lung Microvascular Endothelium cells transfected with pRSV-T 5A (HuLEC-5a or HuLECs) are a cell line derived from the pulmonary endothelium of a human male and subsequently transfected with a PBR322 based plasmid containing the coding region for SV40 in order to immortalise them.[1][2]
HuLECs are used as a laboratory model for the study of the function and pathology of the pulmonary endothelium to research conditions such as ARDS,[3] Tuberculosis,[4] and Interstitial lung disease.[5]
HuLECs are used due to their relatively cheap cost compared to other models of the pulmonary endothelium such as HLMVECs, as well as their hardiness, and their ability to indefinitely proliferate in a laboratory setting.
When fully confluent, HuLECs exhibit a cobblestone phenotype just as they do when lining vessel walls.[2]
References
- ↑ Ades., Candal., Swerlick., George., Summers., Bosse., Lawley. (December 1992). "HMEC-1: establishment of an immortalized human microvascular endothelial cell line". Journal of Investigative Dermatology 99 (6): 683–690. doi:10.1111/1523-1747.ep12613748. PMID 1361507.
- ↑ 2.0 2.1 "HULEC-5a". 2 December 2024. https://thesoundofvinyl.us/blogs/vinyl-101/difference-between-a-record-and-albumhttps://www.atcc.org/products/crl-3244https://www.atcc.org/products/crl-3244.
- ↑ Fakir, Sigdel, Sarker, Folahan, Barabutis (2024). "Ceapin-A7 suppresses the protective effects of Octreotide in human and bovine lung endothelial cell". Cell Stress Chaperones 30 (1): 1–8. doi:10.1016/j.cstres.2024.12.001. PMID 39631560.
- ↑ Mehta, Karls, White, Ades, Quinn (2006). "Entry and intracellular replication of Mycobacterium tuberculosis in cultured human microvascular endothelial cells". Microbial Pathogenesis 41 (2–3): 119–124. doi:10.1016/j.micpath.2006.05.002. PMID 16860530. https://www.sciencedirect.com/science/article/abs/pii/S0882401006000763.
- ↑ Zhang, Jia, Hou, Zhao, Zhang, Tong, Mo, Zhang (2024). "Regulation of Interstitial Lung Diseases by Pulmonary Endothelial Cells via PLVAP". bioRxiv. doi:10.1101/2024.03.12.584592. https://www.biorxiv.org/content/10.1101/2024.03.12.584592v1.article-info.
External links
 |  |
