Biology:Immunodiffusion

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Immunodiffusion
Medical diagnostics
MeSHD005779

Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar[1] which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen.

The commonly known types are:

  1. Single diffusion in one dimension (Oudin procedure)
  2. Double diffusion in one dimension (Oakley Fulthorpe procedure)
  3. Single diffusion in two dimensions (radial immunodiffusion or Mancini method)[2]
  4. Double diffusion in two dimensions (Ouchterlony double immunodiffusion)[3]

Notes

  1. Coleman, R. M.; Kaufman, L. (1972). "Use of the Immunodiffusion Test in the Serodiagnosis of Aspergillosis". Applied Microbiology 23 (2): 301–308. doi:10.1128/am.23.2.301-308.1972. PMID 4622826. 
  2. "Radial Immunodiffusion" (photograph). Edvotek, Inc.. 2017. http://www.edvotek.com/273_gallery.png.  Photograph of precipitin circles in a Petri dish during radial immunodiffusion.
  3. "Diffusion Patterns". Immunodiffusion principles and application. https://image.slidesharecdn.com/immunodiffusion-130121011208-phpapp01/95/immunodiffusion-principles-and-application-17-638.jpg.  Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non-identity.

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