Biology:Leaf protein concentrate

From HandWiki
Leaf protein concentrate (Leafu) made from stinging nettles

Leaf protein concentrate (LPC) refers to the proteinaceous mass extracted from leaves. It can be a lucrative source of low-cost and sustainable protein for food as well as feed applications. Although the proteinaceous extracts from leaves have been described as early as 1773 by Rouelle,[1] large scale extraction and production of LPC was pioneered post the World War II. In fact, many innovations and advances made with regards to LPC production occurred in parallel to the Green Revolution.[2] In some respects, these two technologies were complimentary in that the Green Revolution sought to increase agrarian productivity through increased crop yields via fertiliser use, mechanisation and genetically modified crops, while LPC offered the means to better utilise available agrarian resources through efficient protein extraction.[3]

Sources

Over the years, numerous sources have been experimented. Pirie [4] and Telek[5] described LPC production using a combination of pulping and heat coagulation. Leaves are typically sourced from shrubs or agricultural wastes given their ease of access and relative abundance. Trees are generally considered a poor source of leaf mass for the production of LPC given restrictions on the ease of access. Fallen leaves / leaf litter have negligible protein-content and are of no extractive value.[6]

Plants belonging to the Fabaceae family such as clover, peas and legumes have also been prime candidates for LPC production.[7] While most plants have a mean leaf protein content of 4 to 6% w/v. Fabaceae plants tend to have nearly double that value at 8 to 10% v/w, depending on the protein estimation method employed. Other non-traditional sources include agricultural wastes such as pea (Pisum sativum) pods, cauliflower (Brassica oleracea) leaves, as well as invasive plants such as Gorse (Ulex europeaus), Broom (Cytisus scoparius), and Bracken (Pteridium aquilinum).[8]

Methods of production

LPC production processes are two-staged, with the first focusing on the expression of leaf juice or production of a leaf extract, and the second being the purification or protein recovery stage that recovers protein from the solution.

The most commonly employed method of leaf protein extraction is pulping / juicing.[9][10] Other assisted extraction methods have also been reported such as alkali treatment,[11] pressurised extraction, and enzyme treatment [12] Each method comes with its own advantages although pulping produces the most “native” protein composition and does not require significant investment in complex machinery.

Alkali extraction has been employed with some success [13] although it significantly affects lysine and threonine residues in the protein. Pressurised extraction have limited success. Enzyme treatment is another well reported method which targets the plant cell wall to aid the release of bound proteins. However, enzymes are generally more expensive compared to physical or chemical methods of protein extraction.

Recovering the protein from the extract however is most critical to the nutritive value of the LPC. Commonly reported methods were heat coagulation,[14] acid precipitation,[15] ultrafiltration, solvent precipitation [8] and chromatography.

Heat coagulation is the easiest and the oldest method of protein recovery, albeit the least preferred as most of the nutritive value of the LPC is lost. Acid precipitation is the most commonly employed method of protein recovery although it results in the loss of methionine and tryptophan in the LPC. Ultrafiltration is the most hardware demanding option for protein recovery although it serves more as a protein concentration step rather than complete recovery. Chromatographic methods may be used in tandem with ultrafiltration to help increase solute mass and subsequent recovery. Solvent precipitation is not often reported although it produces the highest protein recovery among other methods and preserves the nutritional integrity of the LPC. The extraction and purification methods are largely inter-compatible and may be employed depending on local facilities. Interestingly, the purity of the final LPC was influenced by the protein content in the initial leaf mass rather than the purification method employed. Furthermore, the amino acid composition of the LPC was dependent on the extraction method employed.[8]

In laboratory conditions, protein fractions of 96% purity could be produced with a recovery of 56% w/w and an overall yield of 5.5%.[12] Telek on the other hand experimented with numerous tropical plants at a large scale using a combination of pulping and heat coagulation. Yields were around 3% with protein recoveries <50%.[16]

Depending on the purity of the recovered protein, they are either called leaf protein extract (<60% w/w), leaf protein concentrate (>60% w/w), or leaf protein isolate (>90% w/w),[17] although publications use these terms interchangeably.

Composition

Whole leaf protein concentrate is a dark green substance with a texture similar to cheese. Approximately 60% of this is water, while the remaining dry matter is 9-11% nitrogen, 20-25% lipid, 5-10% starch and a variable amount of ash. It is a mixture of many individual proteins. Its flavour has been compared to spinach or tea.[18]

Because the colour and taste may make it unpalatable for humans, LPC can instead be separated into green and white fractions. The green fraction has proteins mainly originating from the chloroplasts, while the white fraction has proteins mainly originating from the cytoplasm.[19]

Applications

LPC was first suggested as a human food in the early 20th century, but it has not achieved much success, despite early promise. Norman Pirie, the Copley Medal winner from the UK, studied LPC and promoted its use for human consumption. He and his team developed machines for extraction of LPC, including low-maintenance "village units" intended for poor rural communities. These were installed in places such as villages in south India .[20] The non profit organization, Leaf for Life, maintains a list of human edible leaves and provides recommendations for the top choices of plants.[21]

There has recently been an interest in using LPCs as an alternative food (or resilient food) during times of catastrophe or food shortages.[22] Such resilient food LPCs would be derived from widely geogrphically dispersed tree leaves from forests[23] or agricultural waste.[24]

LPC have been evaluated for infant weaning foods.[25]

The increasing reliance on feedlot based animal rearing to satisfy human appetites for meat has increased demand for cheaper vegetable protein sources. This has recently led to renewed interest in LPC to reduce the use of human-edible vegetable protein sources in animal feed.[26]

Leaf protein has had successful trials as a substitute for soy feed for chickens and pigs.[27]

LPC from alfalfa can be included in feed for tilapia as a partial replacement for fish meal.[28]

Amino Acid Composition

The amino acid composition of the LPC:

Purity of the LPC is expressed as % protein w/w. Amino acid composition (expressed as % w/w protein) of LPC.
Ref Purity Ala Arg Asp Glu Gly His Ile Leu Lys Met Phe Pro Ser Thr Tyr Val
[29] 68.6 6.4 8.3 10.3 12.6 5.7 3.4 5.3 9.7 6.9 2.6 6.6 4.8 2.8 5.7 5.4 6.9
[30] 69.8 6.7 7.8 10.8 13.2 5.9 3.3 5.7 10.2 6.3 2.6 7.0 4.3 3.6 5.9 5.6 7.2
[31] 88.5 5.5 12.7 14.2 26.3 5.6 3.0 4.4 7.8 2.8 2.3 5.8 5.1 5.2 3.4 4.0 6.1
[31] 94.9 5.4 12.3 14.9 28.1 6.0 3.1 4.6 8.0 2.3 2.3 5.9 4.9 5.3 3.6 4.1 6.4
[32] 50.0 5.2 5.4 12.0 10.0 4.5 2.0 4.0 7.8 5.5 1.8 5.3 3.3 4.7 4.2 4.1 5.2
[33] 68.8 4.8 10.0 9.3 15.9 4.7 2.9 4.8 7.3 3.5 3.7 4.8 3.5 3.8 4.2 4.2 6.0
[34] 56.0 4.1 7.1 11.5 21.2 4.2 2.3 5.4 7.6 5.8 1.8 5.5 5.1 5.1 3.4 3.9 5.2
[35] 50.0 4.5 4.6 7.9 11.0 4.4 1.7 4.1 8.4 5.7 1.8 6.1 3.0 3.6 3.7 3.3 5.8
[12] 96.9 7.8 6.0 12.7 12.8 7.6 1.7 2.4 8.6 6.8 2.0 4.9 5.3 6.7 4.0 4.6 3.4
[36] 57.2 6.1 6.6 10.5 11.6 5.5 2.6 6.0 9.7 6.6 1.8 6.4 4.7 4.7 5.3 4.8 7.2
[36] 60.1 6.9 6.5 9.4 11.0 6.0 2.3 6.0 10.1 6.8 2.3 6.8 5.9 4.7 5.0 4.6 6.8
[36] 53.6 6.8 6.4 9.4 11.2 5.9 2.6 6.2 9.9 6.1 2.2 7.0 4.7 4.5 5.6 4.7 7.0
[36] 63.1 6.1 6.6 10.1 11.1 5.4 2.6 6.2 10.0 6.2 2.1 6.6 6.0 4.4 5.3 4.7 6.8
[37] 34.0 3.3 3.1 4.5 5.0 2.9 1.1 2.5 4.5 2.6 1.1 3.0 2.3 1.8 2.3 1.9 3.1
[38] 60.7 3.3 3.8 5.8 6.3 3.1 1.5 2.6 5.2 3.9 1.1 3.4 3.0 2.8 3.1 2.8 3.4
[39] 76.4 5.5 14.2 12.5 11.2 5.1 3.5 4.5 7.1 3.0 1.7 5.4 5.5 5.2 3.6 3.2 5.2
[40] 59.8 5.6 7.2 9.8 12.9 4.7 2.9 4.4 9.7 7.6 2.4 6.3 5.3 3.7 5.5 5.7 6.3
[41] 80.0 10.9 4.9 6.5 23.8 2.2 2.1 4.6 13.1 3.4 2.7 7.7 5.1 5.2 2.8 2.9 5.6
[41] 75.6 9.3 4.8 7.7 22 2.9 2.1 4.6 13.6 3.9 3.1 6.3 5.5 4.6 3.7 2.4 5.8
[42] 83.4 3.7 8.5 12.3 6.4 3.4 2.7 3.8 5.0 8.3 1.7 5.8 3.3 4.0 4.5 4.0 5.6
[43] 95.5 4.0 11.5 7.0 14.5 3.9 2.5 4.0 6.7 2.4 3.2 4.8 5.9 4.3 3.6 3.9 4.9
[43] 97.0 4.2 11.2 8.0 13.5 4.0 2.6 4.1 6.6 2.2 2.1 4.6 5.7 4.1 3.7 3.6 5.2
[44] 46.8 8.3 6.9 13.9 15.9 7.4 2.3 7.0 13.2 8.8 2.9 7.7 6.9 3.7 5.9 6.1 9.3
[45] 58.4 6.3 6.2 9.7 11.3 5.7 3.0 4.6 9.1 6.3 1.1 5.9 4.0 4.3 4.9 5.0 5.6
[46] 55.4 3.4 3.6 5.1 5.9 3.0 1.1 2.3 5.1 2.7 1.3 3.4 2.7 2.6 2.6 2.2 2.8
[47] 46.1 6.0 3.4 11.6 12.4 5.9 1.9 6.3 9.0 2.8 1.9 4.4 4.8 4.3 3.1 4.3 5.5

Dietary issues

Leaf protein is a good source of amino acids, with methionine being a limiting factor.[48] It is nutritionally better than seed proteins and comparable to animal proteins (other than those in egg and milk).[18]

In terms of digestibility, whole LPC has digestibility in the range 65–90%. The green fraction has a much lower digestibility that may be <50%, while the white fraction has digestibility >90%.[19]

The challenges that have to be overcome using lucerne and cassava, two high density monoculture crops, include the high fiber content and other antinutritional factors, such as phytate, cyanide, and tannins.[48]

Lablab beans, Moringa oleifera, tree collards and bush clover may also be used. Flavors of different species vary greatly.[27]

For testing new leaf species for use as LPCs a non-targeted approach has been developed that uses an ultra-high-resolution hybrid ion trap orbitrap mass spectrometer with electrospray ionization coupled to an ultra-high pressure two-dimensional liquid chromatograph system.[49] An open source software toolchain was also developed for automated non‐targeted screening of toxic compounds for LPCs.[50] The process uses three tools 1) mass spectrometry analysis with MZmine 2,[51][52] 2) formula assignment with MFAssignR,[53][54] and 3) data filtering with ToxAssign.[55] Studies have looked at the potential for deciduous trees[49] and coniferous tree leaves.[56] The latter showed yields for LPC extraction from 1% to 7.5% and toxicity screenings confirm that coniferous trees may contain toxins that can be consumed in small amounts, and additional studies including measuring the quantity of each toxin are needed.[56]

See also

References

  1. ↑ Rouelle, Hilaire Marin (1773). "Observations sur les fécules ou parties vertes des plantes, & sur la matiere glutineuse ou végéto animale.". De l'Imprimerie de Vincent.. 
  2. ↑ Pirie, N. W. (1942). "Green Leaves As a Source of Proteins and Other Nutrients" (in en). Nature 149 (3774): 251. doi:10.1038/149251a0. ISSN 0028-0836. 
  3. ↑ Iyer, A. (2021). The revalorisation potential of invasive Scottish plants (ethesis ed.). Aberdeen: University of Aberdeen. pp. 4–12. https://abdn.primo.exlibrisgroup.com/discovery/delivery/44ABE_INST:44ABE_VU1/12198935660005941. 
  4. ↑ Morrison, J. E.; Pirie, N. W. (1961). "The large-scale production of protein from leaf extracts" (in en). Journal of the Science of Food and Agriculture 12 (1): 1–5. doi:10.1002/jsfa.2740120101. https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740120101. 
  5. ↑ Telek, Lehel; Graham, Horace D. (1983). Telek, L.. ed. Leaf Protein Concentrates. Westport, Conn: AVI Publ. Co. ISBN 978-0-87055-412-4. 
  6. ↑ Flindt, Mogens R.; Lillebø, Ana I.; Pérez, Javier; Ferreira, Verónica (2020), Bärlocher, Felix; Gessner, Mark O.; Graça, Manuel A.S., eds., "Total Phosphorus, Nitrogen and Carbon in Leaf Litter" (in en), Methods to Study Litter Decomposition (Cham: Springer International Publishing): pp. 91–105, doi:10.1007/978-3-030-30515-4_11, ISBN 978-3-030-30514-7, http://link.springer.com/10.1007/978-3-030-30515-4_11, retrieved 2023-06-22 
  7. ↑ Pandey, V. N. (1994). "Leaf protein content and yield of some Indian legumes" (in en). Plant Foods for Human Nutrition 46 (4): 313–322. doi:10.1007/BF01088430. ISSN 0921-9668. PMID 7716112. http://link.springer.com/10.1007/BF01088430. 
  8. ↑ 8.0 8.1 8.2 Iyer, Ajay; Bestwick, Charles S.; Duncan, Sylvia H.; Russell, Wendy R. (2021-02-15). "Invasive Plants Are a Valuable Alternate Protein Source and Can Contribute to Meeting Climate Change Targets". Frontiers in Sustainable Food Systems 5. doi:10.3389/fsufs.2021.575056. ISSN 2571-581X. 
  9. ↑ Du, Lin; Arauzo, Pablo J.; Meza Zavala, Maria Fernanda; Cao, Zebin; Olszewski, Maciej Pawel; Kruse, Andrea (2020-01-23). "Towards the Properties of Different Biomass-Derived Proteins via Various Extraction Methods" (in en). Molecules 25 (3): 488. doi:10.3390/molecules25030488. ISSN 1420-3049. PMID 31979336. 
  10. ↑ Makkar, Harinder PS; Francis, George; Becker, Klaus (2008). "Protein concentrate fromJatropha curcas screw-pressed seed cake and toxic and antinutritional factors in protein concentrate" (in en). Journal of the Science of Food and Agriculture 88 (9): 1542–1548. doi:10.1002/jsfa.3248. https://onlinelibrary.wiley.com/doi/10.1002/jsfa.3248. 
  11. ↑ Zhang, Chen; Sanders, Johan P. M.; Xiao, Ting T.; Bruins, Marieke E. (2015-07-22). Mao, Jingdong. ed. "How Does Alkali Aid Protein Extraction in Green Tea Leaf Residue: A Basis for Integrated Biorefinery of Leaves" (in en). PLOS ONE 10 (7): e0133046. doi:10.1371/journal.pone.0133046. ISSN 1932-6203. PMID 26200774. 
  12. ↑ 12.0 12.1 12.2 Iyer, Ajay; Guerrier, Lisa; Leveque, Salomé; Bestwick, Charles S.; Duncan, Sylvia H.; Russell, Wendy R. (2022). "High throughput method development and optimised production of leaf protein concentrates with potential to support the agri-industry" (in en). Journal of Food Measurement and Characterization 16 (1): 49–65. doi:10.1007/s11694-021-01136-w. ISSN 2193-4126. https://link.springer.com/10.1007/s11694-021-01136-w. 
  13. ↑ Zhang, Chen; Sanders, Johan P.M.; Bruins, Marieke E. (2014). "Critical parameters in cost-effective alkaline extraction for high protein yield from leaves" (in en). Biomass and Bioenergy 67: 466–472. doi:10.1016/j.biombioe.2014.05.020. https://linkinghub.elsevier.com/retrieve/pii/S096195341400292X. 
  14. ↑ Ostrowski, Henry T. (1979). "The Isolation of Protein Concentrates From Pasture Herbage and Their Fractionation Into Feed- and Food-Grade Products" (in en). Journal of Food Processing and Preservation 3 (2): 105–124. doi:10.1111/j.1745-4549.1979.tb00575.x. ISSN 0145-8892. 
  15. ↑ Betschart, Antoinette; Kinsella, John E. (1973). "Extractability and solubility of leaf protein" (in en). Journal of Agricultural and Food Chemistry 21 (1): 60–65. doi:10.1021/jf60185a019. ISSN 0021-8561. PMID 4734164. https://pubs.acs.org/doi/abs/10.1021/jf60185a019. 
  16. ↑ Nagy, Steven; Telek, Lehel; Hall, Nancy T.; Berry, Robert E. (1978). "Potential food uses for protein from tropical and subtropical plant leaves" (in en). Journal of Agricultural and Food Chemistry 26 (5): 1016–1028. doi:10.1021/jf60219a028. ISSN 0021-8561. https://pubs.acs.org/doi/abs/10.1021/jf60219a028. 
  17. ↑ Douillard, R.; de Mathan, O. (1994), Hudson, B. J. F., ed., "Leaf protein for food use: potential of Rubisco" (in en), New and Developing Sources of Food Proteins (Boston, MA: Springer US): pp. 307–342, doi:10.1007/978-1-4615-2652-0_10, ISBN 978-1-4613-6139-8, http://link.springer.com/10.1007/978-1-4615-2652-0_10, retrieved 2023-06-22 
  18. ↑ 18.0 18.1 Pirie, N. W. (1966). "Leaf Protein as a Human Food". Science 152 (3730): 1701–1705. doi:10.1126/science.152.3730.1701. ISSN 0036-8075. PMID 5328118. Bibcode: 1966Sci...152.1701P. https://www.jstor.org/stable/1718350. 
  19. ↑ 19.0 19.1 Chiesa, Simone; Gnansounou, Edgard (2011). "Protein extraction from biomass in a bioethanol refinery – Possible dietary applications: Use as animal feed and potential extension to human consumption" (in en). Bioresource Technology 102 (2): 427–436. doi:10.1016/j.biortech.2010.07.125. PMID 20732807. https://linkinghub.elsevier.com/retrieve/pii/S0960852410013453. 
  20. ↑ Fowden, Leslie; Pierpoint, Stan (1997). "Norman Pirie (1907-97)" (in en). Nature 387 (6633): 560. doi:10.1038/42378. ISSN 1476-4687. PMID 9177338. 
  21. ↑ "Top Leaf Crops - Best Food Providers". https://www.leafforlife.org/gen/top-crops.html. 
  22. ↑ Denkenberger, David; Pearce, Joshua M. (2014-11-14) (in en). Feeding Everyone No Matter What: Managing Food Security After Global Catastrophe. Academic Press. ISBN 978-0-12-802358-7. https://books.google.com/books?id=zP2cBAAAQBAJ&pg=PP1. 
  23. ↑ Fist, Tim; Adesanya, Adewale A.; Denkenberger, David; Pearce, Joshua M. (2021). "Global distribution of forest classes and leaf biomass for use as alternative foods to minimize malnutrition" (in en). World Food Policy 7 (2): 128–146. doi:10.1002/wfp2.12030. ISSN 2372-8639. https://onlinelibrary.wiley.com/doi/10.1002/wfp2.12030. 
  24. ↑ Ugwoke, Blessing; Tieman, Ross; Mill, Aron; Denkenberger, David; Pearce, Joshua M. (2023). "Quantifying Alternative Food Potential of Agricultural Residue in Rural Communities of Sub-Saharan Africa" (in en). Biomass 3 (2): 138–162. doi:10.3390/biomass3020010. ISSN 2673-8783. 
  25. ↑ Agbede, J.; Adegbenro, M.; Aletor, O.; Mohammed, A. (2007-09-04). "Evaluation of the nutrition value of Vernonia amygdalina leaf protein concentrates for infant weaning foods" (in en). Acta Alimentaria 36 (3): 387–393. doi:10.1556/aalim.36.2007.3.11. ISSN 1588-2535. https://akjournals.com/view/journals/066/36/3/article-p387.xml. 
  26. ↑ Santamaría-Fernández, Maria; Lübeck, Mette (2020-10-01). "Production of leaf protein concentrates in green biorefineries as alternative feed for monogastric animals". Animal Feed Science and Technology 268: 114605. doi:10.1016/j.anifeedsci.2020.114605. ISSN 0377-8401. https://www.sciencedirect.com/science/article/pii/S0377840120305095. 
  27. ↑ 27.0 27.1 Toensmeier, Eric (2016). The Carbon Farming Solution: A Global Toolkit of Perennial Crops and Regenerative Agriculture Practices for Climate Change Mitigation and Food Security. Chelsea Green Publishing. p. 181. ISBN 978-1-60358-571-2. https://books.google.com/books?id=zsh2CwAAQBAJ. 
  28. ↑ Olvera-Novoa, Miguel A.; Campos, Silvia G.; Sabido, Mirna G.; Martínez Palacios, Carlos A. (1990). "The use of alfalfa leaf protein concentrates as a protein source in diets for tilapia (Oreochromis mossambicus)" (in en). Aquaculture 90 (3–4): 291–302. doi:10.1016/0044-8486(90)90253-J. https://linkinghub.elsevier.com/retrieve/pii/004484869090253J. 
  29. ↑ Betschart, Antoinette; Kinsella, John E. (January 1973). "Extractability and solubility of leaf protein" (in en). Journal of Agricultural and Food Chemistry 21 (1): 60–65. doi:10.1021/jf60185a019. ISSN 0021-8561. PMID 4734164. https://pubs.acs.org/doi/abs/10.1021/jf60185a019. 
  30. ↑ Betschart, Antoinette A. (November 1974). "Nitrogen Solubility of Alfalfa Protein Concentrate As Influenced By Various Factors" (in en). Journal of Food Science 39 (6): 1110–1115. doi:10.1111/j.1365-2621.1974.tb07329.x. ISSN 0022-1147. https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1974.tb07329.x. 
  31. ↑ 31.0 31.1 Betschart, A. A.; Saunders, R. M. (May 1978). "Safflower Protein Isolates: Influence of Recovery Conditions Upon Composition, Yield and Protein Quality" (in en). Journal of Food Science 43 (3): 964–968. doi:10.1111/j.1365-2621.1978.tb02463.x. ISSN 0022-1147. https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1978.tb02463.x. 
  32. ↑ Carlsson, Rof; Hanczakowski, Piotr (October 1985). "The nutritive value of mixtures of white leaf protein and food proteins" (in en). Journal of the Science of Food and Agriculture 36 (10): 946–950. doi:10.1002/jsfa.2740361007. https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740361007. 
  33. ↑ Chee, K.L.; Ling, H.K.; Ayob, M.K. (May 2012). "Optimization of trypsin-assisted extraction, physico-chemical characterization, nutritional qualities and functionalities of palm kernel cake protein" (in en). LWT - Food Science and Technology 46 (2): 419–427. doi:10.1016/j.lwt.2011.12.006. https://linkinghub.elsevier.com/retrieve/pii/S0023643811003999. 
  34. ↑ de Figueiredo, Vitória Ribeiro Garcia; Yamashita, Fábio; Vanzela, André Luis Laforga; Ida, Elza Iouko; Kurozawa, Louise Emy (April 2018). "Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara" (in en). Journal of Food Science and Technology 55 (4): 1508–1517. doi:10.1007/s13197-018-3067-4. ISSN 0022-1155. PMID 29606765. 
  35. ↑ Fantozzi, Paolo; Sensidoni, Alessandro (1983). "Protein extraction from tobacco leaves: technological, nutritional and agronomical aspects" (in en). Qualitas Plantarum Plant Foods for Human Nutrition 32 (3–4): 351–368. doi:10.1007/BF01091194. ISSN 0377-3205. http://link.springer.com/10.1007/BF01091194. 
  36. ↑ 36.0 36.1 36.2 36.3 Horigome, Takao; Kim, Jong Kyu; Uchida, Senji (1983). "Nutritive quality of leaf proteins coagulated at different pH." (in en). Journal of Nutritional Science and Vitaminology 29 (5): 611–620. doi:10.3177/jnsv.29.611. ISSN 0301-4800. PMID 6663367. http://www.jstage.jst.go.jp/article/jnsv1973/29/5/29_5_611/_article. 
  37. ↑ Kammes, K.L.; Bals, B.D.; Dale, B.E.; Allen, M.S. (February 2011). "Grass leaf protein, a coproduct of cellulosic ethanol production, as a source of protein for livestock" (in en). Animal Feed Science and Technology 164 (1–2): 79–88. doi:10.1016/j.anifeedsci.2010.12.006. https://linkinghub.elsevier.com/retrieve/pii/S0377840110004268. 
  38. ↑ Lu, C.D.; Jorgensen, N.A.; Straub, R.J.; Koegel, R.G. (July 1981). "Quality of Alfalfa Protein Concentrate with Changes in Processing Conditions During Coagulation" (in en). Journal of Dairy Science 64 (7): 1561–1570. doi:10.3168/jds.S0022-0302(81)82726-9. 
  39. ↑ Makkar, Harinder PS; Francis, George; Becker, Klaus (July 2008). "Protein concentrate fromJatropha curcas screw-pressed seed cake and toxic and antinutritional factors in protein concentrate" (in en). Journal of the Science of Food and Agriculture 88 (9): 1542–1548. doi:10.1002/jsfa.3248. https://onlinelibrary.wiley.com/doi/10.1002/jsfa.3248. 
  40. ↑ Merodio, Carmen; Sabater, Bartolomé (1988). "Preparation and properties of a white protein fraction in high yield from sugar beet (Beta vulgaris L) leaves" (in en). Journal of the Science of Food and Agriculture 44 (3): 237–243. doi:10.1002/jsfa.2740440305. https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740440305. 
  41. ↑ 41.0 41.1 Mohamed, Tabita Kamara; Zhu, Kexue; Issoufou, Amadou; Fatmata, Tarawalie; Zhou, Huiming (2009-12-01). "Functionality, in Vitro Digestibility and Physicochemical Properties of Two Varieties of Defatted Foxtail Millet Protein Concentrates" (in en). International Journal of Molecular Sciences 10 (12): 5224–5238. doi:10.3390/ijms10125224. ISSN 1422-0067. 
  42. ↑ Purcell, Albert E.; Walter, William M.; Giesbrecht, Francis G. (May 1978). "Protein and amino acids of sweet potato (Ipomoea batatas (L.) Lam.) fractions" (in en). Journal of Agricultural and Food Chemistry 26 (3): 699–701. doi:10.1021/jf60217a051. ISSN 0021-8561. PMID 659720. https://pubs.acs.org/doi/abs/10.1021/jf60217a051. 
  43. ↑ 43.0 43.1 Rivas R., Nilo; Dench, Jane E.; Caygill, John C. (June 1981). "Nitrogen extractability of sesame (Sesamum indicum L.) seed and the preparation of two protein isolates" (in en). Journal of the Science of Food and Agriculture 32 (6): 565–571. doi:10.1002/jsfa.2740320607. https://onlinelibrary.wiley.com/doi/10.1002/jsfa.2740320607. 
  44. ↑ Rosas-Romero, Alfredo; Baratta, Carla (March 1987). "Composition, functional properties, and biological evaluation of a plastein from cassava leaf protein" (in en). Qualitas Plantarum Plant Foods for Human Nutrition 37 (1): 85–96. doi:10.1007/BF01092304. ISSN 0377-3205. http://link.springer.com/10.1007/BF01092304. 
  45. ↑ Smith, Elizabeth B.; Pena, Patricia M. (May 1977). "Use ofF Tetrahymena pyriformis W to Evaluate Protein Quality of Leaf Protein Concentrates" (in en). Journal of Food Science 42 (3): 674–676. doi:10.1111/j.1365-2621.1977.tb12576.x. ISSN 0022-1147. https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2621.1977.tb12576.x. 
  46. ↑ Virabalin, Rajanee; Kositup, B.; Punnapayak, H. (1993). "Leaf protein concentrate from water hyacinth". J. Aquat. Plant Manage. 31: 207–209. 
  47. ↑ Zhang, Yu; Chen, Haixia; Zhang, Ning; Ma, Lishuai (February 2015). "Antioxidant and functional properties of tea protein as affected by the different tea processing methods" (in en). Journal of Food Science and Technology 52 (2): 742–752. doi:10.1007/s13197-013-1094-8. ISSN 0022-1155. PMID 25694682. 
  48. ↑ 48.0 48.1 Hussein, Laila; El-Fouly, Mohamed; El-Baz, F. K.; Ghanem, S. A. (1999-01-01). "Nutritional quality and the presence of anti-nutritional factors in leaf protein concentrates (LPC)". International Journal of Food Sciences and Nutrition 50 (5): 333–343. doi:10.1080/096374899101067. ISSN 0963-7486. PMID 10719564. 
  49. ↑ 49.0 49.1 Pearce, Joshua M.; Khaksari, Maryam; Denkenberger, David (May 2019). "Preliminary Automated Determination of Edibility of Alternative Foods: Non-Targeted Screening for Toxins in Red Maple Leaf Concentrate" (in en). Plants 8 (5): 110. doi:10.3390/plants8050110. ISSN 2223-7747. PMID 31027336. 
  50. ↑ Breuer, S.W.; Toppen, L.; Schum, S.K.; Pearce, J.M. (2021). "Open source software toolchain for automated non‐targeted screening for toxins in alternative foods". MethodsX 8: 101551. doi:10.1016/j.mex.2021.101551. ISSN 2215-0161. PMID 34754818. PMC 8563852. https://doi.org/10.1016/j.mex.2021.101551. 
  51. ↑ Pluskal, Tomáš; Castillo, Sandra; Villar-Briones, Alejandro; Orešič, Matej (2010-07-23). "MZmine 2: Modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data". BMC Bioinformatics 11 (1): 395. doi:10.1186/1471-2105-11-395. ISSN 1471-2105. PMID 20650010. 
  52. ↑ "MZmine 3". https://mzmine.github.io/. 
  53. ↑ Schum, Simeon K.; Brown, Laura E.; Mazzoleni, Lynn R. (2020-12-01). "MFAssignR: Molecular formula assignment software for ultrahigh resolution mass spectrometry analysis of environmental complex mixtures". Environmental Research 191: 110114. doi:10.1016/j.envres.2020.110114. ISSN 0013-9351. https://www.sciencedirect.com/science/article/pii/S0013935120310112. 
  54. ↑ Schum, Simeon (2023-06-21), MFAssignR, https://github.com/skschum/MFAssignR, retrieved 2023-09-03 
  55. ↑ Breuer, Samuel (2021-08-03) (in en). Open Source Software Toolchain for Automated Non-Targeted Screening for Toxins in Alternative Foods. https://osf.io/nh76z/. 
  56. ↑ 56.0 56.1 Mottaghi, Maryam; Meyer, Theresa K.; Tieman, Ross John; Denkenberger, David; Pearce, Joshua M. (2023). "Yield and Toxin Analysis of Leaf Protein Concentrate from Common North American Coniferous Trees" (in en). Biomass 3 (2): 163–187. doi:10.3390/biomass3020011. ISSN 2673-8783. 

Bibliography

  1. Pirie, N. W (1971). "Leaf protein:its agronomy, preparation, quality and use". IBP Handbook. 20. Blackwell Scientific Publications. 
  2. Pirie, N. W (1975). "Leaf protein: a beneficiary of tribulations". Nature 253 (5489): 239–241. doi:10.1038/253239a0. Bibcode: 1975Natur.253..239P. 



Retrieved from "https://handwiki.org/wiki/index.php?title=Biology:Leaf_protein_concentrate&oldid=3627558"