Biology:MAPRE1
 Generic protein structure example |
Microtubule-associated protein RP/EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene.[1][2][3][4]
Function
The protein encoded by this gene was first identified by its binding to the APC (Adenomatous polyposis coli) protein which is often mutated in familial and sporadic forms of colorectal cancer.[5]
Immunofluorescence has demonstrated that EB1 localizes to the centrosome, mitotic spindle, and distal tips of cytoplasmic microtubules. Throughout the cell cycle, EB1 proteins situate on the microtubule plus ends, which is why EB1 is categorized as a microtubule plus end tracking protein(+TIP protein).[6]
The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.[4]
Interactions
MAPRE1 has been shown to interact with TERF1.[7]
References
- ↑ "APC binds to the novel protein EB1". Cancer Res. 55 (14): 2972–7. Aug 1995. PMID 7606712.
- ↑ "The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules". Proc. Natl. Acad. Sci. USA 95 (18): 10596–601. Sep 1998. doi:10.1073/pnas.95.18.10596. PMID 9724749. Bibcode: 1998PNAS...9510596B.
- ↑ "Critical role for the EB1 and APC interaction in the regulation of microtubule polymerization". Curr. Biol. 11 (13): 1062–7. Jul 2001. doi:10.1016/S0960-9822(01)00297-4. PMID 11470413.
- ↑ 4.0 4.1 "Entrez Gene: MAPRE1 microtubule-associated protein, RP/EB family, member 1". https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=22919.
- ↑ "APC binds to the novel protein EB1". Cancer Research 55 (14): 2972–7. July 1995. PMID 7606712. http://cancerres.aacrjournals.org/content/55/14/2972.
- ↑ "EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability". The Journal of Cell Biology 149 (4): 761–6. May 2000. doi:10.1083/jcb.149.4.761. PMID 10811817.
- ↑ "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle". FEBS Lett. 514 (2–3): 193–8. Mar 2002. doi:10.1016/S0014-5793(02)02363-3. PMID 11943150.
Further reading
- "EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability.". J. Cell Biol. 149 (4): 761–6. 2000. doi:10.1083/jcb.149.4.761. PMID 10811817.
- "EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle.". Oncogene 17 (26): 3471–7. 1999. doi:10.1038/sj.onc.1202247. PMID 10030671.
- "EB/RP gene family encodes tubulin binding proteins.". Int. J. Cancer 81 (2): 275–84. 1999. doi:10.1002/(SICI)1097-0215(19990412)81:2<275::AID-IJC18>3.0.CO;2-Z. PMID 10188731.
- "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.". Curr. Biol. 9 (8): 425–8. 1999. doi:10.1016/S0960-9822(99)80190-0. PMID 10226031.
- "EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue.". Oncogene 19 (2): 210–6. 2000. doi:10.1038/sj.onc.1203308. PMID 10644998.
- "Regulation and function of the interaction between the APC tumour suppressor protein and EB1.". Oncogene 19 (15): 1950–8. 2000. doi:10.1038/sj.onc.1203498. PMID 10773885.
- "The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules.". Curr. Biol. 10 (14): 865–8. 2000. doi:10.1016/S0960-9822(00)00600-X. PMID 10899006.
- "The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor.". Cell 107 (3): 347–59. 2001. doi:10.1016/S0092-8674(01)00538-4. PMID 11701125.
- "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle". FEBS Lett. 514 (2–3): 193–8. 2002. doi:10.1016/S0014-5793(02)02363-3. PMID 11943150.
- "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome.". Mol. Biol. Cell 13 (10): 3627–45. 2003. doi:10.1091/mbc.E02-01-0061. PMID 12388762.
- "EB1 targets to kinetochores with attached, polymerizing microtubules.". Mol. Biol. Cell 13 (12): 4308–16. 2003. doi:10.1091/mbc.E02-04-0236. PMID 12475954.
- "The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.". Mol. Biol. Cell 14 (4): 1405–17. 2003. doi:10.1091/mbc.E02-03-0155. PMID 12686597.
- "Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1).". J. Biol. Chem. 278 (38): 36430–4. 2003. doi:10.1074/jbc.M305773200. PMID 12857735.
- "Characterization of functional domains of human EB1 family proteins.". J. Biol. Chem. 278 (50): 49721–31. 2004. doi:10.1074/jbc.M306194200. PMID 14514668.
- "Reconstitution of a microtubule plus-end tracking system in vitro". Nature 450 (7172): 1100–5. 2007. doi:10.1038/nature06386. PMID 18059460. Bibcode: 2007Natur.450.1100B.
- "EBs recognize a nucleotide-dependent structural cap at growing microtubule ends". Cell 149 (2): 371–82. 2012. doi:10.1016/j.cell.2012.02.049. PMID 22500803.
- "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells". PLOS ONE 7 (12): e51442. 2012. doi:10.1371/journal.pone.0051442. PMID 23251535. Bibcode: 2012PLoSO...751442N.
- "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels.". Nat. Cell Biol. 15 (6): 688–93. 2013. doi:10.1038/ncb2744. PMID 23666085.
- "Phosphoregulation of the dimerization and functions of end-binding protein 1". Protein & Cell 5 (10): 795–799. 2014. doi:10.1007/s13238-014-0081-9. PMID 25048701.
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