Biology:Master mix (PCR)
From HandWiki
Short description: Mixture used in molecular biology
A master mix is a mixture containing precursors and enzymes used as an ingredient in RT-PCR techniques in molecular biology. Such mixtures contain a mixture dNTPs (required as a substrate for the building of new DNA strands), MgCl2, Taq polymerase (an enzyme required to building new DNA strands), a pH buffer and come mixed in nuclease-free water.[1][2][3][4]
Master mixes for real-time PCR include a fluorescent compound (frequently SYBR green), and the choice of mix also influence test sensitivity and consistency.[5]
Differences in the choice of master mixes can sometimes explain difference in experimental results, a particular case being the measurement of telomere length.[6][7]
References
- ↑ "PCR Master Mix" (in en). Merck. https://www.sigmaaldrich.com/life-science/molecular-biology/pcr/pcr-master-mix.html.
- ↑ "GoTaq® G2 Master Mixes | PCR Master Mix". https://www.promega.co.uk/products/pcr/taq-polymerase/pcr-master-mix/?catNum=M7822.
- ↑ "PCR Master Mix | Bio-Rad". Bio Rad. https://www.bio-rad.com/featured/en/pcr-master-mix.html.
- ↑ "PCR Master Mixes | Chai". Chai. https://www.chaibio.com/pcr-master-mix.
- ↑ Yang, Jianxin; Kemps-Mols, Berit; Spruyt-Gerritse, Marijke; Anholts, Jacqueline; Claas, Frans; Eikmans, Michael (4 June 2016). "The source of SYBR green master mix determines outcome of nucleic acid amplification reactions". BMC Research Notes 9 (1). doi:10.1186/s13104-016-2093-4. PMID 27259280.
- ↑ Jiménez, Karen M.; Forero, Diego A. (5 April 2018). "Effect of master mixes on the measurement of telomere length by qPCR". Molecular Biology Reports 45 (4): 633–638. doi:10.1007/s11033-018-4175-y.
- ↑ Lin, Jue; Smith, Dana L.; Esteves, Kyle; Drury, Stacy (January 2019). "Telomere length measurement by qPCR – Summary of critical factors and recommendations for assay design". Psychoneuroendocrinology 99: 271–278. doi:10.1016/j.psyneuen.2018.10.005. PMID 30343983.
 |  |
