Biology:Nucleobindin 1

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

Nucleobindin-1 (NUCB1), also known as calnuc, is a protein that in humans is encoded by the NUCB1 gene.

Structure

The human calnuc protein contains 461 amino acids and has a pI of 4.9.

The protein contains the following regions and domains:

• Signal peptide
• Leucine-zipper The leucine-zipper of calnuc may be responsible for its dimerization.
• aa 347-389 – Nuclear localization signal
• 2 zinc binding sites with high affinity (K_d = 20 nM)
• carboxypeptidase-like motifs : HFREXnH (aa 66) and HFTEXnH (aa 146)
• 2 EF-hands
• Potential anchor region– 15-aa hydrophobic region at its COOH terminus that may be sufficient for membrane anchoring

Further reading

• "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. 1992. doi:10.1016/S0006-291X(05)81503-7. PMID 1520323. 
• "Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system". FEBS Lett. 373 (2): 155–8. 1995. doi:10.1016/0014-5793(95)01031-9. PMID 7589456. 
• "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. 1994. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• Ballif BA; Mincek NV; Barratt JT et al. (1996). "Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein". Proc. Natl. Acad. Sci. U.S.A. 93 (11): 5544–9. doi:10.1073/pnas.93.11.5544. PMID 8643612. Bibcode: 1996PNAS...93.5544B. 
• Suzuki Y; Yoshitomo-Nakagawa K; Maruyama K et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Kubota T; Miyauchi M; Miura K et al. (1998). "Upregulation of nucleobindin expression in human-activated lymphocytes and non-Hodgkin's lymphoma". Pathol. Int. 48 (1): 22–8. doi:10.1111/j.1440-1827.1998.tb03823.x. PMID 9589460. 
• Lin P; Le-Niculescu H; Hofmeister R et al. (1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMID 9647645. 
• Taniguchi N; Taniura H; Niinobe M et al. (2000). "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm". J. Biol. Chem. 275 (41): 31674–81. doi:10.1074/jbc.M005103200. PMID 10915798. 
• Weiss TS; Chamberlain CE; Takeda T et al. (2002). "Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 14961–6. doi:10.1073/pnas.261572098. PMID 11752444. 
• Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M. 
• Ota T; Suzuki Y; Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• "Structural studies on the Ca2+-binding domain of human nucleobindin (calnuc)". Biochemistry 43 (31): 10039–49. 2004. doi:10.1021/bi049310a. PMID 15287731. 
• Breuza L; Halbeisen R; Jenö P et al. (2004). "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46". J. Biol. Chem. 279 (45): 47242–53. doi:10.1074/jbc.M406644200. PMID 15308636. 
• Gerhard DS; Wagner L; Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334. 
• Rual JF; Venkatesan K; Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. Bibcode: 2005Natur.437.1173R. 
• Chen Y; Lin P; Qiu S et al. (2007). "Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection". Int. J. Oncol. 30 (5): 1137–44. doi:10.3892/ijo.30.5.1137. PMID 17390015. https://escholarship.org/content/qt28w235xn/qt28w235xn.pdf?t=lnq5wg. 
• Tsukumo Y; Tomida A; Kitahara O et al. (2007). "Nucleobindin 1 controls the unfolded protein response by inhibiting ATF6 activation". J. Biol. Chem. 282 (40): 29264–72. doi:10.1074/jbc.M705038200. PMID 17686766.