Biology:SQ1002

From HandWiki
SQ1002_RNA
RF03533-rscape.svg
Consensus secondary structure and sequence conservation of Clostridioides difficile sRNA SQ1002
Identifiers
SymbolSQ1002_RNA
RfamRF03533
Other data
RNA typeGene; sRNA
GO0070928
SO0000370
PDB structuresPDBe

SQ1002 is a 191nt small RNA identified in Clostridioides difficile 630 by RNA deep sequencing and confirmed by Northern blot and RT-PCR.[1] Extremities were confirmed by 5’ and 3’-RACE.

This RNA is found in several C. difficile strains at syntenic positions between a putative acyltransferase gene and a ferrous iron transport gene. No homologs were found outside C. difficile, although a putative paralog was found in C. difficile 630, located between a tryptophanyl-tRNA synthetase gene and a pseudogene.

Due to lack of sequence variation, the proposed secondary structure is not supported by any covariation and should be considered speculative.

References

  1. Soutourina OA, Monot M, Boudry P, Saujet L, Pichon C, Sismeiro O (2013). "Genome-wide identification of regulatory RNAs in the human pathogen Clostridium difficile.". PLOS Genet 9 (5): e1003493. doi:10.1371/journal.pgen.1003493. PMID 23675309. 

External links