Biology:Total oxyradical scavenging capacity
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Short description: Method of quantifying antioxidant capacity
A total oxyradical scavenging capacity (TOSC) assay is a method of quantifying antioxidant capacity in the context of molecular biology[1] and toxicology, as an indication of oxidative stress.[2] The assay measures ethylene (ethene).[3][4]
The invention of this assay was first made public during 1998.[4]
See also
- Oxygen radical absorbance capacity (ORAC)
References
- ↑ Garrett, A.; Murray, B.; Robison, R.; O'Neill, K. (30 September 2009). "Measuring Antioxidant Capacity Using the ORAC and TOSC Assays". in Donald Armstrong. Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology. 594. Totowa, NJ.: Humana Press. doi:10.1007/978-1-60761-411-1_17. ISBN 978-1-60761-410-4. https://link.springer.com/protocol/10.1007/978-1-60761-411-1_17.
- ↑ Regoli, Francesco (October 2000). "Total oxyradical scavenging capacity (TOSC) in polluted and translocated mussels: a predictive biomarker of oxidative stress". Aquatic Toxicology (Ancona, Italy: Elsevier B.V.) 50 (4): 351–361. doi:10.1016/S0166-445X(00)00091-6. ISSN 0166-445X. PMID 10967397. Bibcode: 2000AqTox..50..351R. https://www.sciencedirect.com/science/article/abs/pii/S0166445X00000916.
- ↑ Winston, G; Regoli, F; Dugas Jr, A; Fong, J; Blanchard, K (1998). "A rapid gas chromatographic assay for determining oxyradical scavenging capacity of antioxidants and biological fluids.". Free Radic Biol Med 24: 480–493. PMID 9438561. https://pubmed.ncbi.nlm.nih.gov/9438561/. "Peroxyl radicals generated by thermal homolysis of 2,2'-azobis-amidinopropane (ABAP) cause the oxidation of alpha-keto-gamma-methiolbutyric acid (KMBA) to ethylene; ethylene formation is monitored by gas chromatographic analysis of head space from the reaction vessel.".
- ↑ 4.0 4.1 Senti T. Senthilmohan; Brett M Davis; Paul F. Wilson; Murray J. McEwan (2009). "Improved peroxyl radical scavenging TOSC assay to quantify antioxidant capacity using SIFT-MS". Redox Report 14 (5): 198. doi:10.1179/135100009X12525712409571. https://www.tandfonline.com/doi/pdf/10.1179/135100009X12525712409571. "The TOSC assay (which is an example of a HAT assay) was introduced by Winston et al.11 in 1998".
Further reading
- Ramona Lichtenthäler; Friedhelm Marx (December 1, 2004). "Total Oxidant Scavenging Capacities of Common European Fruit and Vegetable Juices". J. Agric. Food Chem. (American Chemical Society) 53 (1): 103–110. doi:10.1021/jf0307550. https://pubs.acs.org/doi/10.1021/jf0307550.
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