Biology:TraJ-II RNA motif
| traJ-II RNA | |
|---|---|
 Consensus secondary structure of traJ-II RNAs | |
| Identifiers | |
| Symbol | traJ-II |
| Rfam | RF01760 |
| Other data | |
| RNA type | Cis-regulatory element |
| Domain(s) | Pseudomonadota |
| PDB structures | PDBe |
The traJ-II RNA motif is a conserved RNA structure discovered in bacteria by using bioinformatics.[1] traJ-II RNAs appear to be in the 5' untranslated regions of protein-coding genes called traJ, which functions in the process of bacterial conjugation. A previously identified motif known as TraJ 5' UTR is also found upstream of traJ genes functions as the target of FinP antisense RNAs, so it is possible that traJ-II RNAs play a similar role as targets of an antisense RNA. However, some sequence features within the traJ-II RNA motif suggest that the biological RNA might be transcribed from the reverse-complement strand. Thus is it unclear whether traJ-II function as cis-regulatory elements. traJ-II RNAs are found in a variety of Pseudomonadota.
It was later observed[2] that traJ-II RNAs overlap a previously established oriT (Origin of transfer) plasmid sequence,[3][4] thus suggesting that the traJ RNA motif might function as an oriT.
References
- ↑ "Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea and their metagenomes". Genome Biol 11 (3): R31. March 2010. doi:10.1186/gb-2010-11-3-r31. PMID 20230605.
- ↑ "Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae". Front. Microbiol. 9: 2489. October 23, 2018. doi:10.3389/fmicb.2018.02489. PMID 30405558.
- ↑ "TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence repetition within the transfer origin". J. Biol. Chem. 264 (20): 11989–11994. July 1989. doi:10.1016/S0021-9258(18)80164-8. PMID 2663846.
- ↑ "In vitro assembly of relaxosomes at the transfer origin of plasmid RP4". Proc. Natl. Acad. Sci. U.S.A. 87 (17): 6555–6559. September 1990. doi:10.1073/pnas.87.17.6555. PMID 2168553. Bibcode: 1990PNAS...87.6555P.
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