Biology:UBE2M

Short description: Protein-coding gene in the species Homo sapiens

Generic protein structure example

NEDD8-conjugating enzyme Ubc12 is a protein that in humans is encoded by the UBE2M gene.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Interactions

UBE2M has been shown to interact with NEDD8, PRKAR1A and UBA3.

Further reading

• "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway". J. Biol. Chem. 274 (17): 12036–42. 1999. doi:10.1074/jbc.274.17.12036. PMID 10207026. 
• "The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons". J. Biol. Chem. 275 (12): 8929–35. 2000. doi:10.1074/jbc.275.12.8929. PMID 10722740. 
• "A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo". J. Biol. Chem. 275 (22): 17008–15. 2000. doi:10.1074/jbc.275.22.17008. PMID 10828074. 
• Strausberg RL; Feingold EA; Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932. Bibcode: 2002PNAS...9916899M. 
• "Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif". J. Biol. Chem. 278 (12): 10048–54. 2003. doi:10.1074/jbc.M212632200. PMID 12522145. 
• Huang DT; Miller DW; Mathew R et al. (2004). "A unique E1–E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8". Nat. Struct. Mol. Biol. 11 (10): 927–35. doi:10.1038/nsmb826. PMID 15361859. 
• Gerhard DS; Wagner L; Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334. 
• Huang DT; Paydar A; Zhuang M et al. (2005). "Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1". Mol. Cell 17 (3): 341–50. doi:10.1016/j.molcel.2004.12.020. PMID 15694336. 
• Li T; Santockyte R; Shen RF et al. (2006). "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers". Arch. Biochem. Biophys. 453 (1): 70–4. doi:10.1016/j.abb.2006.03.002. PMID 16620772. https://zenodo.org/record/1258674. 
• Ewing RM; Chu P; Elisma F et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMID 17353931.